UniSTS Help

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Primer Information

Each unique primer pair corresponds to one UniSTS ID that is unique and stable over time.

"PCR product size" displays a summary of all size data submitted from data sources.

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Cross References

The Gene connection is established when any of the following conditions are met:

e-PCR shows a match between an STS and an mRNA sequence that belongs to a gene in entrez Gene database.
STS is located within a gene region, defined as spanning 2 Kb upstream of the 5' end to the 0.5 kb downstream of 3' end, in the genome annotation.

The UniGene connection is made based on e-PCR results on mRNAs and ESTs.

The dbSNP connection is made when SNP is located within the STSs.

RHDB, MGI, RGD and GDB links are made based on data obtained from these external databases.

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Mapping Information

All mapping information available for a given STS is shown.

Publications and data sources for each map are listed in UniSTS home page.

The "Sequence" map indicates an STS map on the sequenced genome that was computed using e-PCR.

Clicking the "Map Viewer" button opens a graphical display showing the position of the marker on the chromosome using the Map Viewer.

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Electronic PCR Result

The e-PCR program is run allowing one mismatch, one gap in each primer alignment, and a size difference of +/- 50 bp between expected size and actual size (N = 1, G = 1 M = 50).