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Library Info Page
| Library Name: | NCI_CGAP_CLL1 |
| Organism: | Homo sapiens |
| UniGene Library ID: | 1317 |
| Tissue Description: | Blood, chronic lymphocytic leukemia cells. PolyA+ mRNA prepared from the peripheral blood of five patients using magnetic cell sorting for CD19+ cells. |
| Library Keywords: | B-cell, chronic lymphocytic leukemia, uncharacterized tissue, flow-sorted, normalized, CGAP, EST, Soares normalized, unknown developmental stage, phagemid, oligo-dT primed |
| #Clones Generated to Date: | 22272 |
| #Sequences Generated to Date: | 13690 |
| Library Preparation Details |
| Description: | 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo. |
| R. Site1: | Not I |
| R. Site2: | Eco RI |
| Lab Host: | DH10B |
| Vector: | pT7T3D-PacI |
| Vector Type: | phagemid (ampicillin resistant) |
| Tissue Supplier: | Ash Alizadeh, John Byrd, M.D., Mike Grever, M.D., Louis M. Staudt, M.D., Ph.D. |
| Library Producer: | Soares Laboratory |
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