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Library Info Page
| Library Name: | NCI_CGAP_Br2 |
| Organism: | Homo sapiens |
| UniGene Library ID: | 895 |
| Tissue Description: | Breast, 3 pooled tumors, including broad spectrum of breast tumor types |
| Library Keywords: | mammary gland, bulk, normalized, CGAP, EST, Soares normalized, unknown developmental stage, female, phagemid, oligo-dT primed, breast (mammary gland) cancer |
| #Clones Generated to Date: | 13440 |
| #Sequences Generated to Date: | 7519 |
| Library Preparation Details |
| Description: | 1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. This library is the normalized version of NCI_CGAP_Br1.1. Library was constructed by Bento Soares and M. Fatima Bonaldo. |
| R. Site1: | |
| R. Site2: | |
| Lab Host: | DH10B |
| Vector: | pT7T3D-PacI |
| Vector Type: | phagemid (ampicillin resistant) |
| Tissue Supplier: | Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. |
| Library Producer: | Soares Laboratory |
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