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Library Info Page
| Library Name: | Soares_placenta_8to9weeks_2NbHP8to9W |
| Organism: | Homo sapiens |
| UniGene Library ID: | 368 |
| Tissue Description: | Placenta, two pooled, one 8 weeks, one 9 weeks post conception. |
| Library Keywords: | normal, non-normalized, placenta, bulk, pregnant, adult, CGAP, EST, Soares non-normalized, female, phagemid, oligo-dT primed |
| #Clones Generated to Date: | 17664 |
| #Sequences Generated to Date: | 15722 |
| Library Preparation Details |
| Description: | 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library constructed by Bento Soares and M.Fatima Bonaldo. |
| R. Site1: | Not I |
| R. Site2: | Eco RI |
| Lab Host: | DH10B (ampicillin resistant) |
| Vector: | pT7T3D (Pharmacia) with a modified polylinker |
| Vector Type: | phagemid |
| Tissue Supplier: | |
| Library Producer: | Soares Laboratory |
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