Award Abstract #0412711
BE/GENEN: Global Transcriptional Responses in Nitrogen Cycling and Nutrient Removal Processes and Development of Supplemental Instructional Workshops

NSF Org:	CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems
Initial Amendment Date:	September 21, 2004
Latest Amendment Date:	September 21, 2004
Award Number:	0412711
Award Instrument:	Standard Grant
Program Manager:	Patrick L. Brezonik CBET Division of Chemical, Bioengineering, Environmental, and Transport Systems ENG Directorate for Engineering
Start Date:	October 1, 2004
Expires:	September 30, 2009 (Estimated)
Awarded Amount to Date:	$1996860
Investigator(s):	Daniel Arp arpd@science.oregonstate.edu (Principal Investigator) Peter Bottomley (Co-Principal Investigator) Lewis Semprini (Co-Principal Investigator)
Sponsor:	Oregon State University OREGON STATE UNIVERSITY Corvallis, OR 97331 541/737-4933
NSF Program(s):	BE-MAT USE:SCIENCE,ENG&SOCIETY, ENVIRONMENTAL GENOMICS
Field Application(s):
Program Reference Code(s):	EGCH, 9187, 1179
Program Element Code(s):	1794, 1693

ABSTRACT

0412711

Arp

This proposal is focused on an important problem in microbial systems used for wastewater treatment. The model organism selected for study is the only ammonia-oxidizing bacterium with a sequenced genome. Transcriptional responses would be evaluated for both planktonic and biofilm cultures. Reporter strains of this organism would also be generated for potential use in wastewater treatment plants as biosensors of potentially deleterious conditions. The proposed research would use the nitrifying bacterium N. europaea as a model for the effects of environmental stress on gene expression. This would be enabled by the complete genome sequence and the subsequent availability of whole genome microarrays. The goal is to identify genes that would serve as markers of stress in wastewater systems. If successful, this approach could be applied to other environmental systems.

The educational plan is well developed, including interdisciplinary training of graduate students and involving high school students. Workshops are to be developed and offered to high school and community college teachers.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

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Gvakharia, BO; Permina, EA; Gelfand, MS; Bottomley, PJ; Sayavedra-Soto, LA; Arp, DJ.  "Global transcriptional response of Nitrosomonas europaea to chloroform and chloromethane,"  APPLIED AND ENVIRONMENTAL MICROBIOLOGY,  v.73,  2007,  p. 3440 - 3445.  


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