Technical Abstract: Twelve pumpkin cultivars (Cucurbita maxima D.), cultivated in Iowa, were studied for their seed oil content, fatty acid composition and tocopherol content. Oil content ranged from 11 to 31%. Total unsaturated fatty acid content ranged from 73.1-80.5%. The predominant fatty acids present were linoleic, oleic, palmitic and stearic. Significant differences were observed among the cultivars for stearic, oleic, linoleic and gadoleic acid content of oil. Low linolenic acid levels were observed (< 1%). Tocopherol content of the oils ranged from 29.9-75.1 micrograms/grams oil for alpha-tocopherol, 74.9-492.8 micrograms/grams for gamma-tocopherol and 35.3-1109.7 micrograms/grams for delta-tocopherol. Study showed potential for pumpkin seed oil from all twelve cultivars to have high oxidative stability that would be suitable for food and industrial applications, as well as high unsaturation and tocopherol content that could potentially improve nutrition of human diets.