ARS | Publication request: Progress Toward a Gene Map for Rainbow Trout (Oncorhynchus Mykiss)

Submitted to: Meeting Abstract
Publication Type: Abstract
Publication Acceptance Date: February 1, 2006
Publication Date: June 30, 2006
Citation: Gharbi, K., Coulibaly, I., Rexroad III, C.E., Moghadam, H., Leder, E.H., Ng, S., Davidson, W., Ferguson, M., Danzmann, R. 2006. Progress toward a gene map for rainbow trout (oncorhynchus mykiss). Meeting Abstract International Symposium for Genetics in Aquaculture 6/25-30, 2006 Montpellier, France.

Technical Abstract: The identification of genes influencing complex traits in fish and other aquaculture species requires a high-density map that provides information on the relative positions of potential candidate genes. In rainbow trout (Oncorhynchus mykiss), considerable progress has been made in the mapping of anonymous genetic markers such as microsatellites and AFLPs, but significant efforts are still needed to document the location of functional genes in relation to other markers in the genome. To this end, we are using two complementary approaches that aim at increasing the gene content of the existing linkage map. The first approach consists in using single strand conformation polymorphism (SSCP) and other mutation detection techniques to identify polymorphic sites in specific genes known to be involved in complex traits of interest, including development, growth, reproduction, and osmoregulation. Our second approach is a generic strategy based on the use of microsatellite repeats identified in public collections of expressed sequence tags (ESTs) as a source of genetic markers. Thus far, the targeted and generic approaches combined have resulted in the addition of more than 250 genes to the rainbow trout map, with new markers being added on a continuing basis. Another application of this resource is to enable comparative genomics between rainbow trout and other vertebrates, including humans, zebrafish, and Fugu. Comparative maps are currently being generated with these species using similarity and database searches to identify regions of conserved syntenies in rainbow trout, and possibly other salmonid fishes.