Technical Abstract: Biotechnology provides the tools to develop industrial products for modern society that would not be possible in any other way (Brownback, 1993). A biotechnological approach to improve sunflower was initiated in 2001 in a collaborative research project that involved genetic transformation of sunflower. Development of transgenic sunflowers creates concern about the possible flow of transgenes into wild sunflower populations, where these populations might be genetically altered in a detrimental way (Arias and Rieseberg, 1994; Whitton, et al., 1997; Faure, et al., 2002; Reagon and Snow, 2006). Thus, pollen from transgenic sunflowers must be contained to prevent the hybridization of transgenic sunflower with wild sunflower. Concerns about biotechnology and gene flow from transgenic crops into their wild relatives have been studied and discussed by numerous researchers and organizations including Council for Agricultural Science and Technology (2001); Daniell, H. (2002); Ellstrand et al. (1999); Ellstand (2002), and Wolfenbarger (2002). A comprehensive risk assessment and mitigation plan includes the physical containment of transgenic plants and their pollen. The objective of this article is to present the standard operating protocol (SOP) we developed and have used for several years for growing transgenic sunflower plants in controlled, contained environments.