Technical Abstract: Lesquerella fendleri is a promising new crop whose seed contains hydroxy fatty acid triglycerides with potential industrial uses. In addition to the oil, lesquerella seed has substantial amounts of valuable gums. Hexane defatted seed has more than 30% protein content. Defatted lesquerella meal was extracted sequentially with 0.5M sodium chloride (2x), water, 70% ethanol, and 0.1 N sodium hydroxide (2x). Each sodium chloride extract was dialyzed against deionized water and centrifuged to separate water-soluble fraction (albumin) from salt-soluble fraction (globulin) before freeze drying. Ethanol extract and neutralized sodium hydroxide extracts (glutelin) were dialyzed against water and freeze dried. Albumin had the highest proportion of lysine and sulfur amino acids per 16 g nitrogen among all the fractions analyzed. Glutelin and globulin accounted for the highest amount of protein nitrogen. SDS-PAGE of reduced albumin, globulin, and glutelin showed the presence of several protein bands with MW ranging from 7 to 98 kD. Nitrogen solubility of defatted lesquerella meal from pH 2 to 12 indicated a minimum solubility of 15% around pH 4.2. Nonprotein nitrogen of defatted meal was 12% of total nitrogen.