Project Number: 5302-22000-008-25
Project Type: Trust

Start Date: Nov 01, 2007
End Date: Oct 31, 2008

Objective:
The overall goal of this proposed research project is to create a system to assist identification, differentiation and fingerprinting of Ca. L. spp. 1. Analyze available phenotypic and genotypic data to estimate population diversity of Ca. L. spp.; 2. Characterize population diversity of Ca. L. spp. based on current genomic information by using a large number of Ca. L. spp. strains; and 3. Search for new phenomic and genomic information and their applications in Ca. L. spp. differentiation and fingerprinting.

Approach:
HLB bacterial DNA will be obtained through collaboration. In phase 1, an extensive literature database on HLB will be constructed. Specific emphasis will be on linking available phenotypic data to more recent genomic discoveries. The second phase is to explore the use of single nucleotide polymorphisms (SNPs) to cluster Ca. L. asiaticus strains. Correlations will be built to link SNPs to geographic and host origins, and other phenotypes such as symptomatology. Efforts will be made to isolate and characterize new DNA sequences from the Ca. L. asiaticus genome for SNP analysis. New data will be added to increase sensitivity and accuracy of the HLB pathogen fingerprinting system. Documents Trust with California Citrus Research Board. Log 34658.