Projects

DEVELOPMENT OF AN INTEGRATED RISK MODEL FOR FOODBORNE ZOONOTIC PARASITES IN SWINE Appropriated (D)   Accession Number: 409642

T. SPIRALIS DETECTION USING MUSCLE LARVAE E/S ANTIGENS IN AN ELISA Reimbursable (R)   Accession Number: 411429

NATIONAL ANIMAL HEALTH MONITORING SURVEY 2006, TOXOPLASMA GONDII AND TRICHINELLA SPIRALIS Reimbursable (R)   Accession Number: 411186

ANALYST TRAINING AND CHECK SAMPLE PROGRAM FOR PORK AND HORSEMEAT EXPORT PROGRAM Reimbursable (R)   Accession Number: 410219

SURVIVAL OF NORTH AMERICAN GENOTYPES OF TRICHINELLA IN FROZEN PORK Reimbursable (R)   Accession Number: 411939

Publications

Complete validation of a unique digestion assay to detect Trichinella larvae in horsemeat demonstrates its reliability for meeting food safety and trade requirements. - (Peer Reviewed Journal)

Forbes, L.B., Hill, D.E., Parker, S., Tessaro, S.V., Gamble, H.R., Gajadhar, A.A. 2008. Complete validation of a unique digestion assay to detect Trichinella larvae in horsemeat demonstrates its reliability for meeting food safety and trade requirements. Journal of Food Protection. 71(3):558-63.

Toxoplasma gondii infection in lambs: high prevalence of live parasites, and genetic characterisation of T. gondii isolates revealed new genotypes - (Peer Reviewed Journal)

Dubey, J.P., Sundar, N., Hill, D.E., Velmurugan, G.V., Bandini, L.A., Kwok, O.C., Su, C. 2008. Toxoplasma gondii infection in lambs: high prevalence of live parasites, and genetic characterisation of T. gondii isolates revealed new genotypes. International Journal for Parasitology. 38:999-1006.

Neosporosis in Beagle dogs clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum. - (Peer Reviewed Journal)

Dubey, J.P., Vianna M, C.R., Kwok, O.C., Hill, D.E., Miska, K.B., Tuo, W., Velmurugan, G.V., Conors, M., Jenkins, M.C. 2007. Neosporosis in Beagle dogs clinical signs, diagnosis, treatment, isolation and genetic characterization of Neospora caninum. Veterinary Parasitology. 149:158-166.

Fecal and serological survey of Neospora caninum in farm dogs in Costa Rica - (Peer Reviewed Journal)

Palavicini, P., Romero, J.J., Dolz, G., Jiminez, A.E., Hill, D.E., Dubey, J.P. 2007. Fecal and serological survey of Neospora caninum in farm dogs in Costa Rica. Veterinary Parasitology. 149:265-270.

Endemic toxoplasmosis in pigs on a farm in Maryland: Isolation and genetic characterization of toxoplasma gondii. - (Peer Reviewed Journal)

Dubey, J.P., Hill, D.E., Sundar, N., Velmurugan, G.V., Bandini, L.A., Kwok, O.C., Pierce, V., Kelly, K., Dulin, M., Su, C. 2008. Endemic toxoplasmosis in pigs on a farm in Maryland: Isolation and genetic characterization of toxoplasma gondii. Journal of Parasitology. 94:36-41.

TOXOPLASMA GONDII - (Review Article)

Hill, D.E. 2007. Toxoplasma gondii in CAB international Animal Health Production, Compendium 2007. Review Article. p. 2887-2991.

Larval viability and serological response in horses with long term Trichinella infection. - (Peer Reviewed Journal)

Hill, D.E., Forbes, L., Kramer, M.H., Gajadhar, A., Gamble, H.R. 2007. Parasitological and immunological parameters of long term infection with Trichinella spiralis (Tl) in the horse, Equus caballus. Veterinary Parasitology. 146(1-2):107-116.

Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue. - (Peer Reviewed Journal)

Hill, D.E., Forbes, L., Gajadhar, A.A., Gamble, H.R. 2007. Viability and infectivity of Trichinella spiralis muscle larvae in frozen horse tissue. Veterinary Parasitology. 146(1-2):102-106.

NEOSPORA CANINUM DETECTED IN WILD RODENTS - (Peer Reviewed Journal)

Jenkins, M.C., Parker, C.C., Hill, D.E., Pinckney, R.D., Dubey, J.P. 2007. Neospora caninum detected in wild rodents. Veterinary Parasitology. 143:161-165.

TOXOPLASMA GONDII - (Book/Chapter)

Hill, D.E., Sreekumar, C., Jones, J., Dubey, J.P. 2007. Toxoplasma gondii. In 'Foodborne Diseases'. S. Simjee, Ed. Humana Press, Totowa, N.J. Book Chapter. pp. 337-353.

TIME AND TEMPERATURE EFFECTS ON T GONDII TISSUE CYSTS IN ENHANCED MEATS. THE EFFECT OF TIME AND TEMPERATURE ON THE VIABILITY OF TOXOPLASMA GONDII TISSUE CYSTS IN ENHANCED PORK LOIN - (Peer Reviewed Journal)

Hill, D.E., Benedetto, S.M., Coss, C., Mccrary, J.L., Fournet, V.M., Dubey, J.P. 2006. Time and temperature effects on T. gondii tissue cysts in enhanced meats; the effect of time and temperature on the viability of Toxoplasma gondii tissue cysts in enhanced pork loin. Journal of Food Protection. 69: 1961-1965.

COMPARISON OF DETECTION METHODS FOR TOXOPLASMA GONDII IN NATURALLY AND EXPERIMENTALLY INFECTED SWINE - (Peer Reviewed Journal)

Hill, D.E., Sreekumar, C., Dubey, J.P., Lunney, J.K., Gamble, H.R. 2006. Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine. Veterinary Parasitology. 141:9-17.

A TRICHINELLA MURRELLI INFECTION IN A DOMESTIC DOG IN THE UNITED STATES - (Peer Reviewed Journal)

Dubey, J.P., Hill, D.E., Zarlenga, D.S. 2006. A trichinella murrelli infection in a domestic dog in the united states. Veterinary Parasitology. 137(3-4): 374-378.

PREVALENCE OF VIABLE TOXOPLASMA GONDII IN BEEF, CHICKEN AND PORK FROM RETAIL MEAT STORES IN THE UNITED STATES; RISK ASSESSMENT TO CONSUMERS - (Popular Publication)

Hill, D.E., Dubey, J.P. 2005. Prevalence of viable toxoplasma gondii in beef, chicken and pork from retail meat stores in the United States; risk assessment to consumers. National Hog Farmer. p. 24.

GENETIC AND BIOLOGIC CHARACTERISTICS OF TOXOPLASMA GONDII ISOLATES IN FREE-RANGE CHICKENS FROM COLUMBIA, SOUTH AMERICA - (Peer Reviewed Journal)

Dubey, J.P., Gomez, J.E., Bedoya, A., Lora, F., Vianna, M., Hill, D.E., Kwok, O.C., Shen, S.K., Marcet, P.L., Lehmann, T. 2005. Genetic and biologic characteristics of toxoplasma gondii isolates in free-range chickens from Columbia, South America. Veterinary Parasitology. 134:67-72.

TRICHINELLA PSEUDOSPIRALIS FROM A WILD PIG IN TEXAS - (Peer Reviewed Journal)

Gamble, H.R., Pozio, E., Lichtenfels, J.R., Zarlenga, D.S., Hill, D.E. 2005. Trichinella pseudospiralis from a wild pig in Texas. Veterinary Parasitology. 132:147-150.

DIFFERENTIAL DETECTION OF HAMMONDIA HAMMONDI FROM TOXOPLASMA GONDII USING POLYMERASE CHAIN REACTION - (Peer Reviewed Journal)

Sreekumar, C., Vianna, M., Hill, D.E., Miska, K.B., Lindquist, A., Dubey, J.P. 2005. Differential detection of hammondia hammondi from toxoplasma gondii using polymerase chain reaction. Parasitology International. 54(4):267-269.

PREVALENCE OF VIABLE TOXOPLASMA GONDII IN BEEF, CHICKEN, AND PORK FROM RETAIL MEAT STORES IN THE UNITED STATES AND RISK ASSESMENT TO CONSUMERS - (Peer Reviewed Journal)

Dubey, J.P., Hill, D.E., Jones, J.L., Hightower, A.W., Kirkland, E., Roberts, J.M., Marcet, P.L., Lehmann, T., Vianna, M., Miska, K.B., Sreekumar, C., Kwok, O.C., Shen, S.K., Gamble, H.R. 2005. Prevalence of viable toxoplasma gondii in beef, chicken, and pork from retail meat stores in the united states and risk assesment to consumers. Journal of Parasitology 91:1082-1093.

GENOTYPING AND DETECTION OF MULTIPLE INFECTIONS OF TOXOPLASMA GONDII USING PYROSEQUENCING - (Peer Reviewed Journal)

Sreekumar, C., Hill, D.E., Miska, K.B., Vianna, M.B., Yan, L., Myers, R.L., Dubey, J.P. 2005. Genotyping and detection of multiple infections of Toxoplasma gondii using pyrosequencing. International Journal for Parasitology. 35:991-995.

A COMPARISON OF FOUR SEROLOGIC TESTS TO DETECT ANTIBODIES TO TOXOPLASMA GONDII IN NATURALLY-EXPOSED BOTTLENOSE DOLPHINS (TURSIOPS TRUNCATUS). - (Peer Reviewed Journal)

Dubey, J.P., Fair, P.A., Bossart, G.D., Hill, D.E., Fayer, R., Sreekumar, C., Kwok, O.C., Thulliez, P. 2005. A comparison of four serologic tests to detect antibodies to toxoplasma gondii in naturally-exposed bottlenose dolphins (tursiops truncatus). Journal of Parasitology. 91:1074-1081.

ISOLATION OF NEOSPORA CANINUM FROM NATURALLY INFECTED WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) - (Peer Reviewed Journal)

Vianna, M.C., Sreekumar, C., Miska, K.B., Hill, D.E., Dubey, J.P. 2005. Isolation of Neospora caninum from naturally infected white-tailed deer (Odocoileus virginianus). Veterinary Parasitology. 129:253-257.

TOXOPLASMA GONDII - (Review Article)

Hill, D.E., Chirukandoth, S., Dubey, J.P. 2005. Toxoplasma gondii. Animal Health Research Reviews. 6(1):41-61.

TRICHINHELLA NATIVA (T2) IN A BLACK BEAR FROM PLYMUTH, NEW HAMPSHIRE - (Peer Reviewed Journal)

Hill, D.E., Gamble, H.R., Zarlenga, D.S., Coss, C.A., Finnigan, J. 2005.Trichinhella nativa (t2) in a black bear from Plymouth, New Hampshire. Veterinary Parasitology. 132; 143-146.

MEAT PROCESSING TECHNOLOGIES MAY HELP BLOCK TOXOPLASMA TRANSMISSION - (Trade Journal)

Hill, D.E. 2004. Meat processing technologies may help block toxoplasma transmission. National Hog Farmer. p. 31

ISOLATION, TISSUE DISTRIBUTION, AND MOLECULAR CHARACTERIZATION OF TOXOPLASMA GONDII FROM CHICKENS FROM GRENADA, WEST INDIES. - (Peer Reviewed Journal)

Dubey, J.P., Bhalyat, M.I., Miska, K.B., Macpherson, C.N., Sharma, R.N., Sreekumar, C., Hill, D.E., Shen, S.K., Kwok, O.C., Lehmann, T. 2004. Isolation, tissue distribution, and molecular characterization of toxoplasma gondii from chickens from Grenada, West Indies. Journal of Parasitology. 91(3):557-560.

THE EFFECT OF COMMONLY USED ENHANCEMENT SOLUTIONS ON THE VIABILITY OF TOXOPLASMA GONDII TISSUE CYSTS IN PORK LOIN - (Peer Reviewed Journal)

Hill, D.E., Sreekuman, C., Gamble, H.R., Dubey, J.P. 2004. The effect of commonly used enhancement solutions on the viability of toxoplasma gondii tissue cysts in pork loin. Journal of Food Protection. 67:2230-2233.

BIOLOGIC, MORPHOLOGIC AND MOLECULAR CHARACTERIZATION OF NEOSPORA CANINUM ISOLATES FROM LITTERMATE DOGS - (Abstract)

Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M., Kwok, O.C., Hill, D.E., Jenkins, M.C., Lindsay, D.S., Greene, C.E. 2004. Biologic, morphologic and molecular characterization of neospora caninum isolates from littermate dogs (Abstract). COST 454 Annual Conference, Protozoal Reproduction Losses in Farm Ruminants. p.1.

TOXOPLASMOSIS - (Book/Chapter)

Dubey, J.P., Hill, D.E., Sreekumar, C. 2005. Toxoplasmosis, in Kahn, C.M., eds. The Merck Veterinary Manual nineth editiion, Publication Services, Merck and Co., White House Station, New Jersey, 549 p.

SHEDDING OF NEOSPORA CANINUM OOCYSTS BY DOGS FED TISSUES FRO NATURALY INFECTED WATER BUFFALOES (BUBALUS BUBALIS) FROM BRAZIL.- - (Peer Reviewed Journal)

Rodrigues, A.R., Gennari, S.M., Aguiar, D.M., Sreekumar, C., Hill, D.E., Miska, K.B., Vianna, M.C., Dubey, J.P. 2004. Shedding of Neospora caninum oocysts by dogs fed tissues from naturaly infected water buffaloes (Bubalus bubalis) from Brazil. Veterinary Parasitology. 124:139-150.

CLINICAL, BIOLOGIC, MORPHOLOGIC, AND MOLECULAR CHARACTERISATION OF NEOSPORA CANINUM INFECTIONS IN LITTERMATE DOGS - (Peer Reviewed Journal)

Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M.C., Kwok, O.C., Hill, D.E., Jenkins, M.C., Lindsay, D.S., Greene, C.E. 2004. Clinical, biologic, morphologic, and molecular characterisation of neospora caninum infections in littermate dogs. International Journal for Parasitology 34:1157-1167.

HAMMONDIA HEYDORNI: EVIDENCE OF GENETIC DIVERITY AMONG ISOLATES FROM DOGS. - (Peer Reviewed Journal)

Sreekumar, C., Hill, D.E., Miska, K.B., Rosenthal, B.M., Vianna, M.B., Venturini, L., Basso, W., Gennan, S.M., Lindsay, D.S., Dubey, J.P. 2004. Hammondia heydorni: evidence of genetic diverity among isolates from dogs. Experimental Parasitology. 107:65-71.

FIRST ISOLATION OF NEOSPORA CANINUM FROM AN ABORTED BOVINE FETUS IN SPAIN - (Peer Reviewed Journal)

Canada, N., Meireles, C.S., Mezo, M., Warleta, M., Correia Da Costa, J.M., Sreekumar, C., Hill, D.E., Miska, K.B., Dubey, J.P. 2004. First isolation of neospora caninum from an aborted bovine fetus in spain. Journal of Parasitology 90:863-864.

MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF HAMMONDIA HEYDORNI-LIKE OOCYSTS FROM A DOG FED HEARTS FROM NATURALLY-INFECTED WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) - (Peer Reviewed Journal)

Dubey, J.P., Sreekumar, C., Miska, K.B., Hill, D.E., Vianna, M.C., Lindsay, D.S. 2004. Molecular and biological characterization of Hammondia heydorni-like oocysts from a dog fed hearts from naturally-infected white-tailed deer (Odocoileus virginianus). Journal of Parasitology. 90:1174-1176.

DETECTION OF HAMMONDIA HEYDORNI-LIKE ORGANISMS AND THEIR DIFFERENTIATION FROM NEOSPORA CANINUM USING RAPD-PCR - (Peer Reviewed Journal)

Sreekumar, C., Hill, D.E., Fournet, V.M., Rosenthal, B.M., Lindsay, D.S., Dubey, J.P. 2003. Detection of hammondia heydorni-like organisms and their differentiation from Neospora caninum using RAPD-PCR. Journal of Parasitology. 89:1082-1085.

BESNOITIA ORYCTOFELISI N. SP. (PROTOZOA: APICOMPLEXA) FROM DOMESTIC RABBITS - (Peer Reviewed Journal)

DUBEY, J.P., SREEKUMAR, C., LINDSAY, D.S., HILL, D.E., ROSENTHAL, B.M., VENTURINI, L., VENTURINI, M.C., GREINER, E.C. 2003.BESNOITIA ORYCTOFELISI N. SP. (PROTOZOA: APICOMPLEXA) FROM DOMESTIC RABBITS. PARASITOLOGY 126:521-539.

HIGH PREVALENCE OF VIABLE TOXOPLASMA GONDII INFECTION IN MARKET WEIGHT PIGS FROM A FARM IN MASSACHUSETTS - (Peer Reviewed Journal)

Dubey, J.P., Gamble, H.R., Hill, D.E., Sreekumar, C., Romand, S., Thulliez, P. 2002. High prevalence of viable toxoplasma gondii infection in market weight pigs from a farm in massachusetts. Journal of Parasitology 88:1234-1238.

CONTROL OF IXODES SCAPULARIS AND AMBLYOMMA AMERICANUM USING THE '4-POSTER' TREATMENT DEVICE ON DEER IN MARYLAND - (Proceedings/Symposium)

Carroll, J.F., Allen, P.C., Hill, D.E., Pound, J., Miller, J., George, J. 2002. Control of ixodes scapularis and amblyomma americanum using the '4-poster' treatment device on deer in maryland. Experimental and Applied Acarology. 28:289-296.

PREVALENCE OF ANTIBODIES TO NEOSPORA CANINUM AND SARCOCYSTIS NEURONA IN SERA OF DOMESTIC CATS FROM BRAZIL - (Peer Reviewed Journal)

Dubey, J.P., Lindsay, D.S., Hill, D.E., Romand, S., Thulliez, P., Kwok, O.C., Silva, J., Oliveira-Camargo, M.C., Gennari, S.M. 2002. Prevalence of antibodies to neospora caninum and sarcocystis neurona in sera of domestic cats from brazil. Journal of Parasitology 88:1251-1252.

REDESCRIPTION OF NEOSPORA CANINUM DUBEY, CARPENTER, SPEER, TOPPER, UGGLA, 1988 AND ITS DIFFERENTIATION FROM RELATED COCCIDIA ESPECIALLY ISOSPORA BIGEMINA AND HAMMONDIA HEYDORNI - (Peer Reviewed Journal)

Dubey, J.P., Barr, B.C., Barta, J.R., Bjerkas, I., Bjorkman, C., Blagburn, B.L., Bowman, D.D., Buxton, D., Ellis, J.T., Hill, D.E. 2002. Redescription of neospora caninum dubey, carpenter, speer, topper, uggla, 1988 and its differentiation from related coccidia especially isospora bigemina and hammondia heydorni. International Journal for Parasitology 32:929-946.

IDENTIFICATION AND PARTIAL CHARACTERIZATION OF A CDNA SEQUENCE ENCODING A GLUTAMIC ACID-RICH PROTEIN SPECIFICALLY TRANSCRIBED IN TRICHINELLA SPIRALIS NEWBORN LARVAE AND RECOGNIZED BY SWINE INFECTION SERUM. - (Peer Reviewed Journal)

Zarlenga, D.S., Boyd, P., Lichtenfels, J.R., Hill, D.E., Gamble, H.R. 2002. Identification and partial characterization of a cdna sequence encoding a glutamic acid-rich protein specifically transcribed in trichinella spiralis newborn larvae and recognized by swine infection serum. International Journal for Parasitology.

ESTABLISHMENT OF BESNOITIA DARLINGI FROM OPOSSUMS (DIDELPHIS VIRGINIANA) IN EXPERIMENTAL INTERMEDIATE AND DEFINITIVE HOSTS, PROPAGATION IN CELL CULTURE, AND DESCRIPTION OF ULTRASTRUCTURAL AND GENETIC CHARACTERISTICS - (Peer Reviewed Journal)

Dubey, J.P., Lindsay, D.S., Rosenthal, B.M., Sreekumar, C., Hill, D.E., Shen, S.K., Kwok, O.C., Rickard, L.G., Black, S.S., Rashmir-Raven, A. 2002. Establishment of besnoitia darlingi from opossums (didelphis virginiana) in experimental intermediate and definitive hosts, propagation in cell culture, and description of ultrastructural and genetic characteristics. International Journal for Parasitology 32:1053-1064.