Projects

MOLECULAR CHARACTERIZATION OF PATHOGENS AND THEIR RESPONSES TO ENVIRONMENTAL FACTORS Appropriated (D)   Accession Number: 410797

DEVELOPMENT OF BIOMARKERS IN FOOD-BORNE PATHOGENS Memorandum of Understanding (M)   Accession Number: 410280

PATHOGENIC ESCHERICHIA COLI NETWORK Nonfunded Cooperative Agreement (N)   Accession Number: 411464

ANTIMICROBIAL SURFACE COATINGS TO CONTROL THE GROWTH OF BACTERIA Specific Cooperative Agreement (S)   Accession Number: 409780

Publications

Quorum Sensing in Biofilms: Why Bacteria Behave the Way They Do - (Review Article) - (05-Sep-08)

Evaluation of a Multiplex PCR System for Simultaneous Detection of Salmonella spp., Listeria monocytogenes, and Escherichia coli O157:H7 in Foods and in Food Subjected to Freezing - (Peer Reviewed Journal) - (28-Aug-08)

Sequencing of the Escherichia coli O22 O Antigen Gene Cluster and Detection of E. coli Serogroups O22 and O91 by Multiplex PCR Assays Targeting Virulence Genes and the wzy Gene in the Respective O-Antigen Gene Clusters - (Peer Reviewed Journal) - (04-Aug-08)

CHARACTERIZATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI STRAINS ISOLATED FROM SWINE FECES - (Peer Reviewed Journal) - (22-Jul-08)

The Application of ¿Omics¿ Technologies for Food Safety Research - (Other)

Fratamico, P.M. 2008. The Application of ¿Omics¿ Technologies for Food Safety Research. Foodborne Pathogens and Disease. 5. 369-370

Identification of Salmonella Serotypes Isolated from Cantaloupe and Chile Pepper Production Systems in Mexico Using PCR-RFLP - (Peer Reviewed Journal) - (01-Jun-08)

Animal and Environmental Impact on the Presence and Distribution of Salmonella spp. in Hydroponic Tomato Greenhouses - (Peer Reviewed Journal)

Orozco, L., Iturriaga, M., Tamplin, M., Fratamico, P.M., Call, J.E., Luchansky, J.B., Escartin, E. 2008. Animal and Environmental Impact on the Presence and Distribution of Salmonella spp. in Hydroponic Tomato Greenhouses. Journal of Food Protection. 71(4):676-683.

Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006 - (Peer Reviewed Journal)

Uhlich, G.A., Sinclair, J.R., Warren, N.G., Chmielecki, W.A., Fratamico, P.M. 2008. Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006. Applied and Environmental Microbiology. 74:1268-1272.

Campylobacter: stress responses and biofilm formation - (Proceedings/Symposium)

Fratamico, P.M., Gunther, N.W., Bagi, L.K. 2007. Campylobacter: stress responses and biofilm formation. Meeting Abstract.

SOCIETY FOR GENERAL MICROBIOLOGY M - (Abstract)

Fratamico, P.M. 2007. Society for general microbiology m. Meeting Abstract. P.31.

APPLICATIONS OF THE POLYMERASE CHAIN REACTION (PCR) FOR DETECTION, IDENTIFICATION, AND TYPING OF FOOD-BORNE MICROORGANISMS - (Book/Chapter)

Fratamico, P.M., Kawasaki, S. 2007. Applications of the polymerase chain reaction (pcr) for detection, identification, and typing of food-borne microorganisms. CRC Press, New York. Microbial Food Contamination, C.Wilson 9ed.) 2nd edition. p.213-254

EXTRAINTESTINAL PATHOGENIC ESCHERICHIA COLI (EXPEC) - (Review Article)

Smith, J.L., Fratamico, P.M., Gunther, N.W. 2007. Extraintestinal pathogenic escherichia coli (expec). Foodborne Pathogens and Disease. Vol.4(2):134-163

Molecular Characterization of the Escherichia coli Serogroup O117, O126, and O146 O Antigen Gene Clusters and Development of PCR Assays Targeting Serogroup O117-, O126-, and O146-Specific DNA Sequences - (Peer Reviewed Journal)

Liu, Y., Debroy, C., Fratamico, P.M. 2007. Molecular Characterization of the Escherichia coli Serogroup O117, O126, and O146 O Antigen Gene Clusters and Development of PCR Assays Targeting Serogroup O117-, O126-, and O146-Specific DNA Sequences. Molecular and Cellular Probes. 21:295-302.

COMPARISON OF METHODS FOR DETECTION AND ISOLATION OF COLD-AND FREEZE-STRESSED ESCHERICHIA COLI O157:H7 IN RAW GROUND BEEF - (Peer Reviewed Journal)

Fratamico, P.M., Bagi, L.K. 2007. Comparison of methods for detection and isolation of cold-and freeze-stressed escherichia coli o157:h7 in raw ground beef. Journal of Food Protection. Vol.70(7):1663-1669.

TESTING OF SWINE FECES OBTAINED THROUGH THE NATIONAL ANIMAL HEALTH MONITORING SYSTEM'S SWINE 2000 STUDY FOR THE PRESENCE OF ESCHERICHIA COLI O157:H7 - (Peer Reviewed Journal)

Feder, I.E., Gray, J.T., Pearce, R., Fratamico, P.M., Bush, E., Porto, A., Wallace, F.M., Cray, P.J., Luchansky, J.B. 2007. Testing of swine feces obtained through the national animal health monitoring system's swine 2000 study for the presence of escherichia coli 0157:h7. Journal of Food Protection. Vol. 70(6):1489-1492.

Non-O157 Shiga Toxin-Producing E. coli Associated with Muscle Foods - (Abstract)

Fratamico, P.M. 2007. Non-O157 Shiga Toxin-Producing E. coli Associated with Muscle Foods. Meeting Abstract. P.195.02.

Bacterial quorum sensing and biofilm formation - (Abstract)

Fratamico, P.M. Bacterial quorum sensing and biofilm formation. Meeting Abstract. 041-01.

Applications of omics for food safety and security - (Abstract)

Fratamico, P.M., Luchansky, J.B. 2007. Applications of omics for food safety and security. Meeting Abstract. International Association for Food Protection. S19.

Detection of Escherichia coli O157:H7 in food using a real-time multiplex PCR assay - (Abstract)

Fratamico, P.M., Debroy, C. 2007. Detection of Escherichia coli O157:H7 in food using a real-time multiplex PCR assay. Abstract. Internatinal Association for Food Protection. P3-69.

COMPLETE NUCLEOTIDE SEQUENCES OF 84.5-KB AND 3.2-KB PLASMIDS IN THE MULTI-ANTIBIOTIC RESISTANT SALMONELLA ENTERICA SEROVAR TYPHIMURIUM U302 STRAIN G8430 - (Peer Reviewed Journal)

Chen, C., Nace, G., Solow, B., Fratamico, P.M. 2007. Complete nucleotide sequences of 84.5-kb and 3.2-kb plasmids in the multi-antibiotic resistant salmonella enterica serovar typhimurium u302 strain g8430. Plasmid. 57:29-53.

POTENTIAL SOURCES OF SALMONELLA CONTAMINATION ON TOMATOES GROWN IN HYDROPONIC GREENHOUSES - (Abstract)

Orozco, L.R., Tamplin, M.L., Fratamico, P.M., Call, J.E., Luchansky, J.B., Escartin, E. 2006. Potential sources of salmonella contamination on tomatoes grown in hydroponic greenhouses. [Abstract] International Association for Food Protection's Annual Meeting P4-11. pg. 158.

ESCHERICHIA COLI O ANTIGEN TYPING USING DNA MICROARRAYS - (Abstract)

Liu, Y., Fratamico, P.M. 2006. Escherichia coli o antigen typing using dna microarrays. {Abstract}. International Association of Food Protection. P3-54.

PREVALENCE AND ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES RECOVERED FROM FINISHING PIG HERDS AND SLAUGHTER FACILITIES IN SOUTHERN BRAZIL - (Abstract)

Kich, J., Coldebella, A., Mores, N., Fratamico, P.M., Call, J.E., Luchansky, J.B., Cray, P.J. 2006. Prevalence and antibiotic resistance of salmonella isolates recovered from finishing pig herds and slaughter facilities in southern brazil. {Abstract}. International Association of Food Protection. P2-10. p. 117.

CHARACTERIZATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI STRAINS ISOLATED FROM SWINE FECES - (Abstract)

Fratamico, P.M., Bagi, L.K., Cray, P.J., Bhagwat, A.A. 2006. Characterization of shiga toxin-producing escherichia coli strains isolated from swine feces. {Abstract}. International Association of Food Protection. P1-47.

E. COLI O ANTIGEN TYPING USING DNA MICROARRAYS - (Peer Reviewed Journal)

Liu, Y., Fratamico, P.M. 2006. E. coli o antigen typing using dna microarrays. Molecular and Cellular Probes. 2006. 20:239-244.

CHARACTERIZATION, TYPING, AND DETECTION OF PATHOGENIC ESCHERICHIA COLI - (Proceedings/Symposium)

Fratamico, P.M., Uhlich, G.A., Liu, Y. 2005. Characterization, typing, and detection of pathogenic escherichia coli. UJNR Food & Agricultural Panel Proceedings.

DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI 0145 BY PCR TARGETING GENES IN THE E. COLI 0145 ANTIGEN GENE CLUSTER AND THE SHIGA TOXIN 1 AND SHIGA TOXIN 2 GENES - (Abstract)

Fratamico, P.M., Debroy, C., Miyamoto, T., Liu, Y. 2005. Detection of Enterohemorrhagic Escherichia Coli 0145 by PCR Targeting Genes in the E. Coli 0145 Antigen Gene Cluster and the Shiga Toxin 1 and Shiga Toxin 2 Genes. Association of Official Analytical Chemists. Abstract. Paper # P-611.

DNA SEQUENCE OF THE ESCHERICHIA COLI O103 O ANTIGEN GENE CLUSTER AND DETECTION OF ENTEROHEMORRAGIC E. COLI O103 BY PCR AMPLIFICATION OF THE WZX AND WZY GENES - (Peer Reviewed Journal)

Fratamico, P.M., Debroy, C., Strobaugh Jr, T.P., Chen, C. 2005. Dna sequence of the escherichia coli o103 o antigen gene cluster and detection of enterohemorragic e. coli o103 by pcr amplification of the wzx and wzy genes. Canadian Journal of Microbiology.

PCR ASSAYS FOR DETECTION AND IDENTIFICATION OF ESCHERICHIA COLI SEROGROUPS O45 AND O55 TARGETING THE WZX AND WZY GENES IN THE O ANTIGEN GENE CLUSTERS - (Peer Reviewed Journal)

Debroy, C., Fratamico, P.M., Briggs, C.E., Kundrat, J., Roberts, E., Liu, Y., Davis, M. 2005. Pcr assays for detection and identification of Escherichia coli serogroups O45 and O55 targeting the wzx and wzy genes in the O antigen gene clusters. Applied and Environmental Microbiology. v. 71. p. 4919-4924.

DETECTING PATHOGENS IN CATTLE AND MEAT - (Book/Chapter)

Fratamico, P.M., Gehring, A.G., Karns, J.S., Van Kessel, J.S. 2005. Detecting pathogens in cattle and meat. Book Chapter. Improving the Safety of Fresh Meat, Woodhead Publishing Ltd., Cambridge, UK pg:25-55

REAL-TIME PCR - (Proceedings/Symposium)

Fratamico, P.M. 2005. Real-time pcr. Food Protection Trends. 25:44-46.

MOLECULAR APPROACHES FOR DETECTION, IDENTIFICATION, AND ANALYSIS OF FOOD-BORNE PATHOGENS - (Book/Chapter)

Fratamico, P.M., Bayles, D.O. 2005. Molecular approaches for detection, identification, and analysis of food-borne pathogens. Book Chapter. Foodborne Pathogens: Microbiology and Molecular Biology. Caister Academic Press, Norfolk, Virginia. pg. 1-13.

DIARRHEA-INDUCING ESCHERICHIA COLI - (Book/Chapter)

Smith, J.L., Fratamico, P.M. 2005. Diarrhea-inducing escherichia coli. Book Chapter. Caister Academic Press, Norfolk, UK p. 357-382.

LOOK WHAT'S COMING DOWN THE ROAD: POTENTIAL FOOD BORNE PATHOGENS - (Book/Chapter)

Smith, J.L., Fratamico, P.M. 2005. Look what's coming down the road: potential food borne pathogens. Book Chapter. Foodborne Pathogens: Microbiology & Molecular Biology, Caister Academic Press, Norfolk, UK

NUCLEIC ACID-BASED METHODS FOR DETECTION AND ANALYSIS OF FOODBORNE PATHOGENS - (Proceedings/Symposium)

Fratamico, P.M., Bagi, L.K., Liu, Y., Miyamoto, T. 2004. Nucleic acid-based methods for detection and analysis of foodborne pathogens. United States Japan Natural Resources Protein Panel. p. 348-353.

PREVALENCE AND CHARACTERIZATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI IN SWINE FECES RECOVERED IN THE NATIONAL ANIMAL HEALTH MONITORING SYSTEM'S SWINE 2000 STUDY - (Peer Reviewed Journal)

Fratamico, P.M., Bagi, L.K., Bush, E.J., Solow, B.T. 2004. Prevalence and characterization of shiga toxin-producing Escherichia coli in swine feces recovered in the national animal health monitoring system's swine 2000 study. Applied and Environmental Microbiology. v.70. P. 12.

NUCLEIC ACID-BASED DIAGNOSTIC METHODOLOGIES - (Book/Chapter)

Liu, Y., Fratamico, P.M. 2004. Nucleic acid-based diagnostic methodologies. Book Chapter. Paper No. 6-TECH.

NUCLEIC ACID-BASED METHODS FOR DETECTION AND CHARACTERIZATION OF FOOD-BORNE PATHOGENS - (Abstract)

Fratamico, P.M. 2004. Nucleic acid-based methods for detection and characterization of food-borne pathogens. River Falls Food Microbiology Symposium. Abstract. Paper # 14.

NUCLEIC ACID-BASED DIAGNOSTIC METHODS - (Abstract)

Liu, Y., Fratamico, P.M. 2004. Nucleic acid-based diagnostic methods. Meeting Abstract. P.AGFD168.

COMPROMISED HUMAN HEALTH AND THE INCREASED RISK OF FOODBORNE DISEASE - (Abstract)

Smith, J.L., Tamplin, M.L., Fratamico, P.M. 2004. Compromised human health and the increased risk of foodborne disease. Meeting Abstract. P.19.

ESCHERICHIA COLI - (Book/Chapter)

Fratamico, P.M., Smith, J.L. 2005. Escherichia coli. In Foodborne Infections and Intoxications. D.O. Cliver and H. Reimann (ed.), Academic Press, New York.p. 205-258.

FOOD-BORNE PATHOGENS AND AT-RISK POPULATIONS: MICROBIOLOGICAL CONSIDERATIONS - (Abstract)

Fratamico, P.M., Tamplin, M.L., Smith, J.L. 2004. Food-borne pathogens and at-risk populations: microbiological considerations. Meeting Abstract. P.7

QUORUM SENSING: A PRIMER FOR FOOD MICROBIOLOGISTS - (Review Article)

Smith, J.L., Fratamico, P.M., Novak, J.S. 2003. Quorum sensing: a primer for food microbiologists. Journal Of Food Protection. 67(5):1053-1070.

QUORUM SENSING AND FOOD SAFETY - (Abstract)

Novak, J.S., Fratamico, P.M. 2004. Quorum sensing and food safety. Meeting Abstract. S-11.

DETECTION OF ESCHERICHIA COLI SEROTYPES 026 AND 0113 BY PCR AMPLIFICATION OF THE WZX AND WZY GENES - (Peer Reviewed Journal)

Debroy, C., Roberts, E., Kundrat, J., Davis, M.A., Briggs, C.E., Fratamico, P.M. 2004. Detection of escherichia coli serotypes 026 and 0113 by pcr amplification of the wzx and wzy genes. Applied and Environmental Microbiology. 2004. v. 70(3):1830-1832.

AN EVALUATION OF ASCORBIC ACID AS A QUORUM SENSING ANALOGUE TO CONTROL GROWTH, SPORULATION, AND ENTEROTOXIN PRODUCTION IN CLOSTRIDIUM PERFRINGENS - (Peer Reviewed Journal)

Novak, J.S., Fratamico, P.M. 2004. An evaluation of ascorbic acid as a quorum sensing analogue to control growth, sporulation, and enterotoxin production in clostridium perfringens. Applied And Environmental Microbiology. Vol. 69. Nr. 3. 2004. p.72-78.

DETECTION OF PATHOGENS IN FRUITS AND VEGETABLES - (Proceedings/Symposium)

Fratamico, P.M., Annous, B.A. 2003. Detection of pathogens in fruits and vegetables. NFRI, Japan Meeting Abstract.

DETECTION AND CHARACTERIZATION OF PATHOGENIC ESCHERICHIA COLI - (Proceedings/Symposium)

Fratamico, P.M., Bagi, L.K., Briggs, C.E., Debroy, C. 2003. Detection and characterization of pathogenic escherichia coli. United States Japan Natural Resources Protein Panel. p. 92-98.

REAL-TIME PCR - (Abstract)

FRATAMICO, P.M. REAL-TIME PCR. MEETING ABSTRACT. 2003.

DETECTION OF FOOD-BORNE PATHOGENS BY REAL-TIME POLYMERASE CHAIN REACTION ASSAYS - (Abstract)

FRATAMICO, P.M. DETECTION OF FOOD-BORNE PATHOGENS BY REAL-TIME POLYMERASE CHAIN REACTION ASSAYS. MEETING ABSTRACT. 2003.

CHARACTERIZATION OF TWO PLASMIDS THAT POSSESS ANTIBIOTIC RESISTANCE GENES IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM U302 STRAIN G8430 - (Abstract)

CHEN, C., NACE JR, G.W., BRIGGS, C.E., SOLOW, B., FRATAMICO, P.M. CHARACTERIZATION OF TWO PLASMIDS THAT POSSESS ANTIBIOTIC RESISTANCE GENES IN SALMONELLA ENTERICA SEROVAR TYPHIMURIUM U302 STRAIN G8430. MEETING ABSTRACT. 2003.

COMPARISON OF CULTURE, POLYMERASE CHAIN REACTION (PCR), TAQMAN SALMONELLA, AND TRANSIA CARD SALMONELLA ASSAYS FOR DETECTION OF SALMONELLA SPP. IN NATURALLY-CONTAMINATED GROUND CHICKEN, GROUND TURKEY, AND GROUND BEEF - (Peer Reviewed Journal)

Fratamico, P.M. 2003. Comparison of culture, polymerase chain reaction (pcr), taqman salmonella, and transia card salmonella assays for detection of salmonella spp. in naturally-contaminated ground chicken, ground turkey, and ground beef. Molecular and Cellular Probes. 17(5):215-221.

SEQUENCE OF THE ESCHERICHIA COLI O121 O ANTIGEN GENE CLUSTER AND DETECTION OF E. COLI O121 BY PCR AMPLIFICATION OF THE WZX AND WZY GENES - (Peer Reviewed Journal)

FRATAMICO, P.M., BRIGGS, C.E., NEEDLE, D., CHEN, C., DEBROY, C. Sequence of the Escherichia coli O121 O antigen gene cluster and detection of E. coli O121 by PCR amplification of the wzx and wzy genes. JOURNAL OF CLINICAL MICROBIOLOGY. Vol. 41. No. 7. pg. 3379-3383.

COMPARISON OF METHODS FOR DETECTION AND ISOLATION OF COLD-STRESSED ESCHERICHIA COLI O157:H7 IN RAW GROUND BEEF - (Abstract)

FRATAMICO, P.M., BAGI, L.K. COMPARISON OF METHODS FOR DETECTION AND ISOLATION OF COLD-STRESSED ESCHERICHIA COLI O157:H7 IN RAW GROUND BEEF. MEETING ABSTRACT. 2003.

PREVALENCE AND CHARACTERIZATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI IN SWINE FECES BASED ON THE NATIONAL ANIMAL HEALTH MONITORING SYSTEM'S SWINE 2000 STUDY - (Abstract)

FRATAMICO, P.M., BAGI, L.K., BUSH, E. PREVALENCE AND CHARACTERIZATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI IN SWINE FECES BASED ON THE NATIONAL ANIMAL HEALTH MONITORING SYSTEM'S SWINE 2000 STUDY. MEETING ABSTRACT. 2003.

THE ISOLATION OF ESCHERICHIA COLI 0157:H7 FROM INTACT COLON SAMPLES OF SWINE AT A SWINE SLAUGHTER FACILITY - (Peer Reviewed Journal)

FEDER, I.E., WALLACE, F.M., GRAY, J.T., FRATAMICO, P.M., CRAY, P.J., PEARCE, R., CALL, J.E., PERRINE, R., LUCHANSKY, J.B. THE ISOLATION OF ESCHERICHIA COLI 0157:H7 FROM INTACT COLON SAMPLES OF SWINE AT A SWINE SLAUGHTER FACILITY. EMERGING INFECTIOUS DISEASES. 2003. vol. 9. No. 3.

CAMPYLOBACTER: STRESS RESPONSES IN FOOOD ENVIRONMENTS AND DETECTION. - (Abstract)

FRATAMICO, P.M., BHADURI, S., SOLOW, B., CLOAK, O. CAMPYLOBACTER: STRESS RESPONSES IN FOOOD ENVIRONMENTS AND DETECTION. MEETING ABSTRACT. 2002.

NATIONAL ANIMAL HEALTH MONITORING SYSTEM SWINE 2000: A SURVEILLANCE STUDY OF ESCHERICHIA COLI 0157 IN SWINE - (Abstract)

Feder, I.E., Gray, J.T., Pearce, R., Bush, E., Fratamico, P.M., Wallace, F.M., Cray, P.J., Perrine, R., Dudley, R.L., Luchansky, J.B. 2002. National animal health monitoring system swine 2000: a surveillance study of escherichia coli 0157 in swine. International Association for Food Protection. P. 87. P120.