Projects

IMMUNOLOGIC AND PHARMACOLOGICAL INTERVENTIONS OF VECTOR-BORNE BABESIOSIS Appropriated (D)   Accession Number: 411502

Publications

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle - (Peer Reviewed Journal)

Goff, W.L., Johnson, W.C., Molloy, J.B., Jorgensen, W.K., Waldron, S.J., Figueroa, J.V., Matthee, O., Adams, D.S., Mcguire, T.C., Pino, I., Mosqueda, J., Palmer, G.H., Suarez, C.E., Knowles Jr, D.P., Mcelwain, T.F. 2008. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle. Clinical and Vaccine Immunology. 15(9):1316-1321.

Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG- or Babesia bovis-exposed splenic dendritic cells - (Peer Reviewed Journal)

Bastos, R.G., Johnson, W.C., Mwangi, W., Brown, W.C., Goff, W.L. 2008. Bovine NK cells acquire cytotoxic activity and produce IFN-gamma after stimulation by Mycobacterium bovis BCG- or Babesia bovis-exposed splenic dendritic cells. Veterinary Immunology and Immunopathology. 124:302-312.

Bovine WC1- gamma delta T-cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18 - (Peer Reviewed Journal)

Johnson, W.C., Bastos, R.G., Davis, W.C., Goff, W.L. 2008. Bovine WC1- gamma delta T-cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-gamma in response to exogenous IL-12 and IL-18. Developmental and Comparative Immunology. 32:1002-1010.

Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates - (Peer Reviewed Journal)

Scoles, G.A., Goff, W.L., Lysyk, T.J., Lewis, G.S., Knowles Jr, D.P. 2008. Validation of an Anaplasma marginale cELISA for use in the diagnosis of A. ovis infections in domestic sheep and Anaplasma spp. in wild ungulates. Veterinary Microbiology. 130:184-190.

Differential response of splenic monocytes and DC from cattle to microbial stimulation with Mycobacterium bovis BCG and Babesia bovis merozoites - (Peer Reviewed Journal)

Bastos, R.G., Johnson, W.C., Brown, W.L., Goff, W.L. 2007. Differential response of splenic monocytes and DC from cattle to microbial stimulation with Mycobacterium bovis BCG and Babesia bovis merozoites. Veterinary Immunology and Immunopathology. 115(3-4):334-345.

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis - (Peer Reviewed Journal)

Goff, W.L., Molloy, J.B., Johnson, W.C., Suarez, C.E., Pino, I., Rhalem, A., Sahibi, H., Ceci, L., Carelli, G., Adams, D.S., Mcguire, T.C., Knowles Jr, D.P., Mcelwain, T.F. 2006. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis. Clinical and Vaccine Immunology. 13(11):1212-1216.

IMMUNE CONTROL OF BABESIA BOVIS INFECTION - (Peer Reviewed Journal)

Brown, W.C., Norimine, J., Knowles Jr, D.P., Goff, W.L. 2006. Immune control of babesia bovis infection. Veterinary Parasitology. 138:75-87.

PROSPECTS FOR RECOMBINANT VACCINES AGAINST BOVINE BABESIOSIS. - (Peer Reviewed Journal)

Brown, W.C., Norimine, J., Goff, W.L., Suarez, C.E., Mcelwain, T.F. 2006. Prospects for recombinant vaccines against bovine babesiosis. 2006. Parasite Immunology. 28:315-327.

BOVINE SPLENIC NK CELLS SYNTHESIZE IFN-GAMMA IN RESPONSE TO IL-12-CONTAINING SUPERNATANTS FROM BABESIA BOVIS-EXPOSED MONOCYTE CULTURES - (Peer Reviewed Journal)

Goff, W.L., Storset, A.K., Johnson, W.C., Brown, W.C. 2006. Bovine splenic NK cells synthesize IFN-gamma in response to IL-12-containing supernatants from Babesia bovis-exposed monocyte cultures. Parasite Immunology. 28:221-228.

EXPRESSION OF C-TERMINAL TRUNCATED AND FULL-LENGTH BABESIA BIGEMINA RHOPTRY-ASSOCIATED PROTEIN-1 AND THEIR POTENTIAL USE IN ENZYME-LINKED IMMUNOSORBENT ASSAY. - (Peer Reviewed Journal)

Boonchit, S., Alhassan, A., Chan, B., Xuan, X., Yokoyama, N., Ooshiro, M., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I. 2006. Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay.Veterinary Parasitology. 137(1-2):28-35.

IMPROVED DIAGNOSTICS FOR EQUINE PIROPLASMOSIS SURVEILLANCE AND CONTROL PROGRAMS - (Proceedings/Symposium)

Goff, W.L., Rhalem, A., Lasri, S., Johnson, W.C., Sahibi, H., Knowles Jr, D.P. 2006. Improved diagnostics for equine piroplasmosis surveillance and control programs. 9th Congress of the World Equine Veterinary Association. p.265-266.

COMPARATIVE INFECTIVITY OF BABESIA DIVERGENS AND A ZOONOTIC BABESIA DIVERGENS-LIKE PARASITE IN CATTLE. - (Peer Reviewed Journal)

Holman, P.J., Spencer, A., Telford, S.R., Goethert, H.K., Allen, A., Knowles Jr, D.P., Goff, W.L. 2005. Comparative infectivity of babesia divergens and a zoonotic babesia divergens-like parasite in cattle. American Journal of Tropical Medicine and Hygiene. 73(5):865-870.

POLYPHASIC TAXONOMY - (Review Article)

Uilenberg, G., Goff, W.L. 2006. Polyphasic Taxonomy. Annals of the New York Academy of Sciences. 1081:492-497.

NEW RUMINANT HOSTS AND WIDER GEOGRAPHIC RANGE IDENTIFIED FOR BABESIA ODOCOILEI (EMERSON AND WRIGHT 1970) - (Peer Reviewed Journal)

Schoelkopf, L., Hutchinson, C.E., Bendele, K.G., Goff, W.L., Willette, M., Rasmussen, J.M., Holman, P.J. 2005. New ruminant hosts and wider geographic range identified for babesia odocoilei (emerson and wright 1970). Journal of Wildlife Diseases. 41(4):683-690

IMPROVED ENZYME-LINKED IMMUNOSORBENT ASSAY USING C-TERMINAL TRUNCATED RECOMBINANT ANTIGENS OF BABESIA BOVIS RHOPTRY-ASSOCIATED PROTEIN-1 FOR THE DETECTION OF SPECIFIC ANTIBODIES. - (Peer Reviewed Journal)

Boonchit, S., Xuan, X., Yokoyama, N., Goff, W.L., Waghela, S.D., Wagner, G., Igarashi, I. 2004. Improved Enzyme-Linked Immunosorbent Assay Using C-Terminal Truncated Recombinant Antigens of Babesia bovis Rhoptry-Associated Protein-1 for Detection of Specific Antibodies. Journal of Clinical Microbiology. 42(4):1601-1604.

THE INNATE IMMUNE RESPONSE TO CALVES IN BOOPHILUS MICROPLUS TICK TRANSMITTED BABESIA BOVIS INVOLVES TYPE-1 CYTOKINE INDUCTION AND NK-LIKE CELLS IN THE SPLEEN - (Peer Reviewed Journal)

Goff, W.L., Johnson, W.C., Horn, R.H., Barrington, G.M., Knowles Jr, D.P. 2003. The innate immune response to calves in boophilus microplus tick transmitted babesia bovis involves type-1 cytokine induction and nk-like cells in the spleen. Parasite Immunology. 25:185-188

COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY BASED ON A RHOPTRY-ASSOCIATED PROTEIN 1 EPITOPE SPECIFICALLY IDENTIFIES BABESIA BOVIS-INFECTED CATTLE - (Peer Reviewed Journal)

Goff, W.L., McElwain, T.F., Suarez, C.E., Johnson, W.C., Brown, W.C., Norimine, J., Knowles, D.P. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein-1 epitope specifically identifies Babesia bovis-infected cattle. Clinical and Diagnostic Laboratory Immunology. 2002. v. 10. p. 38-43.

SCIENTIFIC EXCHANGE FOR MOROCCAN TECHNICIANS ENGAGED IN RESEARCH ON TICK-BORNE DISEASE CONTROL IN NORTH AFRICA - (Other)

Jehle, P., Goff, W.L. Scientific exchange for Moroccan technicians engaged in research on tick-borne disease control in North Africa. AgLink U.S.-Morocco Trade Promotion Program-1998-2003. 2003. End of Project Report. p. 49.

EVALUATION OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY WITH RECOMBINANT RHOPTRY-ASSOCIATED PROTEIN-1 ANTIGEN AGAINST BABESIA BOVIS FOR THE DETECTION OF SPECIFIC ANTIBODIES IN CATTLE - (Peer Reviewed Journal)

Boonchit, S., Xuan, X., Yokoyama, N., Goff, W.L., Wagner, G.G., Igarashi, I. 2002. Evaluation of an enzyme-linked immunosorbent assay with recombinant rhoptry-associated protein-1 antigen against babesia bovis for the detection of specific antibodies in cattle. Journal of Clinical Microbiology. 40:3771-3775.