Publications

Gmax-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently Using Engineered Workcell - (Abstract)

Hughes, S.R., Moser, B.R., Doll, K.M., Tasaki, K., Harmsen, A., Jones, M., Bang, S., Gibbons, W., Butt, T. 2009. GMAX-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently using Engineered Workcell [abstract]. Center for Process Analytical Chemisty.

Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA - (Abstract)

Rastogi, G., Muppidi, G., Gurram, R., Adhikari, A., Bang, S., Christopher, L., Bischoff, K.M., Hughes, S.R., Apel, W., Sani, R.K. 2009. Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota [abstract]. Symposium on Biotechnology for Fuels and Chemicals.

Hemicellulolytic enzymes from the maize endophyte Acremonium zeae - (Abstract)

Bischoff, K.M., Jordan, D.B., De Rezende, S.T., Rich, J.O. 2009. Hemicellulolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Biotechnology for Fuels and Chemicals.

Rapid evaluation of fuel ethanol contaminant biofilms - (Abstract)

Rich, J.O., Leathers, T.D., Bischoff, K.M. 2009. Rapid evaluation of fuel ethanol contaminant biofilms [abstract]. 31st Symposium on Biotechnology for Fuels and Chemicals.

Gmax Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically - (Abstract)

Hughes, S.R. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit.

Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone - (Peer Reviewed Journal)

(03-Apr-09)

Integrated Biorefineries with Engineered Microbes and High-value Co-products for Profitable Biofuels Production - (Review Article)

(02-Mar-09)

Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen - (Peer Reviewed Journal)

(19-Feb-09)

Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation - (Abstract)

Yoder, A., Pajak, L., Jackson Jr, J.S., Hughes, S.R. 2009. Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation [abstract]. LabAutomation.

Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract)

Hughes, S.R. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009

Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production - (Peer Reviewed Journal)

(07-Jan-09)

Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae - (Peer Reviewed Journal)

(25-Dec-08)

Modeling bacterial contamination of fuel ethanol fermentation - (Peer Reviewed Journal)

(15-Dec-08)

Make use of lactic acid bacteria in biomass to biofuel - (Abstract)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make use of lactic acid bacteria in biomass to biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology.

Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri - (Peer Reviewed Journal)

(31-Oct-08)

Conversion of biomass to ethanol by other organisms - (Book/Chapter)

Liu, S. 2009. Conversion of biomass to ethanol by other organisms. Letters in Applied Microbiology. 48:337-342.

Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain - (Abstract)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract].

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2008. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61:22-38.

Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks - (Abstract)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting.

Cellulosic ethanol production from Saccharomyces cerevisiae engineered for anaerobic conversion of pretreated lignocellulosic sugars to ethanol - (Abstract)

Rich, J.O., Bischoff, K.M., Hughes, S.R. 2008. Cellulosic ethanol production from Saccharomyces cerevisiae engineered for anaerobic conversion of pretreated lignocellulosic sugars to ethanol [abstract]. American Chemical Society. Paper ID1211547.

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Thermophilic hydrolytic enzymes from the maize endophyte Acremonium zeae - (Abstract)

Bischoff, K.M. 2008. Thermophilic hydrolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Society of Industrial Microbiology Annual Meeting.

Lactobacillus buchneri: A novel biocatalyst for ethanol production - (Abstract)

Liu, S. 2008. Lactobacillus buchneri: A novel biocatalyst for ethanol production [abstract]. MWA Bioenergy Research Forum.

Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology Meeting. 0-030. p. 155.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Biofuels group NSF DUSEL project - (Abstract)

Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels group NSF DUSEL project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.

Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically - (Abstract)

Hughes, S.R., Rich, J.O. 2008. Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically [abstract]. Laboratory Robotics Information Group (LRIG). Seminar 1. p. 1.

Controlled antibiotic use during fuel ethanol production - (Abstract)

Bischoff, K.M., Scott, J., Leathers, T.D., Liu, S., Worthington, R. 2008. Controlled antibiotic use during fuel ethanol production [abstract]. National Science Foundation, Partnerships for Innovation Workshop. Poster No. 13.

Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters - (Peer Reviewed Journal)

Cotterill, I.C., Rich, J.O., Scholten, M.W., Mozhaeva, L., Michels, P.C. 2008. Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters. Biotechnology and Bioengineering. Available: http://www.interscience.wiley.com DOI 10.1002/bit.21929

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.

Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains - (Abstract)

Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.

Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. 3rd International Symposium on Biocatalysis and Biotechnology. p. 49.

Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains - (Abstract)

Hughes, S.R., Chen, H., Tsai, D., Thompson, W., Dowd, P.F., Hooberman, B., Benjamin, L., Alexander, L.J., Rosentrater, K.A., Rich, J.O. 2007. Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains [abstract]. FDA Meeting. Talk 1.

Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms - (Abstract)

Hughes, S.R., Bang, S., Bleakley, B. 2007. Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms [abstract]. Meeting with South Dakota State Mine & Technology (SDSM&T) and South Dakota State University (SDSU). BioDUSEL Section, Day 2, Talk 3. p. 15.

Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Peer Reviewed Journal)

Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2008. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products. Journal of Industrial Microbiology and Biotechnology. 35:75-81.

Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants - (Peer Reviewed Journal)

Bischoff, K.M., Skinner-Nemec, K.A., Leathers, T.D. 2007. Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants. Journal of Industrial Microbiology and Biotechnology. 34(11):739-744.

Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.

Monensin inhibits growth of bacterial contaminants from fuel ethanol plants - (Abstract)

Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.

Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 - (Peer Reviewed Journal)

Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154.

A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Abstract)

Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158.

High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.

Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. - (Abstract)

Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides [abstract]. ACS National Meeting. p. 108.

Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces - (Abstract)

Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces [abstract]. 2007 World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.

Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract)

Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.

Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy. - (Abstract)

Riedmuller, S., Hughes, S.R., Hector, R.E., Bischoff, K.M., Farrelly, P., Cotta, M.A., Li, X. 2007. Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Seminar 43. p. 4.

Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide - (Abstract)

Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.

Developing Gram-Positive Ethanologens - (Abstract)

Liu, S. 2006. Developing gram-positive ethanologens [abstract]. International Symposium of Biocatalysis and Bioenergy. p. 33.

Chromosomal Inactivation of the Als Gene in Lactobacillus Plantarum Strain Tf103 to Eliminate Undesired Fermentation Products - (Abstract)

Liu, S. 2006. Chromosomal inactivation of the als gene in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products [abstract]. Society of Industrial Microbiology. p. 166.

Purification and Characterization of a Family 5 Endoglucanase from a Moderately Thermophilic Strain of Bacillus Licheniformis - (Peer Reviewed Journal)

Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765.

Genetically Engineered Lactic Acid Bacteria for the Production of Fuels and Chemicals - (Abstract)

Liu, S. 2006. Genetically engineered lactic acid bacteria for the production of fuels and chemicals [abstract]. 10th International Symposium on the Genetics of Industrial Microorganisms. Paper #015.

Isolation of Xylose-Utilizing Thermophilic Bacteria from Dairy Manure Compost - (Abstract)

Bischoff, K.M., Liu, S., Edrington, T.S., Hughes, S.R. 2006. Isolation of xylose-utilizing thermophilic bacteria from dairy manure compost [abstract]. Extremophiles 2006. p. 19. Poster P045.

Antimicrobial Susceptibility of Lactobacillus Species Isolated from Fuel-Ethanol Plants - (Abstract)

Bischoff, K.M., Skinner, K.A., Leathers, T.D. 2006. Antimicrobial susceptibility of lactobacillus species isolated from fuel-ethanol [abstract]. American Society for Microbiology. Poster No. O-038.

Functional Proteomic Plasmid-Based Integrated Workcell for High-Throughput Transformation of Bl21 De3 E. Coli for Expression in Vivo with Piromyces Strain Xylose Isomerase - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.

High-Throughput Screening of Cellulase F Mutants from Multiplexed Plasmid Sets Using An Automated Plate Assay on a Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Qureshi, N., Cotta, M.A., Farrelly, P.J. 2006. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell. Proteome Science. 4:10.

A Cold-Sensitive Listeria Monocytogenes Mutant Has a Transposon Insertion in a Gene Encoding a Putative Membrane Protein and Shows Altered (P)ppgpp Levels - (Peer Reviewed Journal)

Liu, S., Bayles, D.O., Mason, T.M., Wilkinson, B.J. 2006. A cold-sensitive Listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein and shows altered (p)ppGpp levels. Applied and Environmental Microbiology. 72(6):3955-3959.

Automated Strategy Using a Functional Proteomic Assay to Identify and Isolate Cellulase F Mutants with Improved Activity from Multiplexed Sets of Plasmid - (Abstract)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.

Mass Transformation of Plasmid Libraries of Cdna Or Mutagenized Clone Sets into Yeast Or Bacteria Using a Functional Proteomic Robotic Workcell - (Abstract)

Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.

Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract)

Hughes, S.R., Mertens, J.A., Li, X., Bischoff, K.M. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Laboratory Robotics Information Group. p. 1.

A Simple Method to Generate Chromosomal Mutations in Lactobacillus Plantarum Strain Tf103 to Eliminate Undesired Fermentation Products - (Peer Reviewed Journal)

Liu, S. 2006. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products. Applied Biochemistry and Biotechnology. 129-132:854-863.

Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.

Functional Proteomic Workcell for High Volume Plasmid Preparations for Repeated in Vitro Protein Expression and High Throughput Screening to Identify Mutant Enyzmes for Use at Low Ph - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.

Antimicrobial Susceptibility of Bacterial Contaminants from a Wet-Mill Ethanol Plant - (Abstract)

Bischoff, K.M., Skinner-Nemec, K., Leathers, T.D., Hughes, S.R. 2005. Antimicrobial susceptibility of bacterial contaminants from a wet-mill ethanol plant [abstract]. Society for Industrial Microbiology. Poster P42.

Characterization of Carboxymethylcellulase Activity from Geobacillus Stearothermophilus - (Abstract)

Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38.

Lactobacillus Brevis: a Potential Biocatalyst for Lignocellulosic Biomass to Ethanol - (Abstract)

Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.

Development of a Liquid Handler Component for a Plasmid-Based Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.

Metabolic Engineering of a Lactobacillus Plantarum Double Ldh Knockout Strain for Enhanced Ethanol Production - (Peer Reviewed Journal)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.

Ethanol Fermentation by Overexpressing a Gram-Positive Pdc Gene in Lactobacillus Plantarum Strain Tf103 - (Abstract)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.

Metabolic Engineering of Lactobacillus Brevis for Ethanol Production - (Abstract)

Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.

Development of a Liquid Handler Component for a Functional Plasmid-Based Proteomic Workcell That Generates Multiplex Samples Expresses in Yeast - (Abstract)

Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.

Modified Liquid Handler Used to Produce High Throughput High Quality Plasmids for Integration into Plasmid-Based Functional Proteomics Workcell - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.

A Plasmid-Based Functional Proteomic Workcell Assay Designed to Screen Orpinomyces Cellulase F Nny Mutagenized Libraries in High Throughtput - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell assay designed to screen Orpinomyces cellulase F NNY mutagenized libraries in high throughtput [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.

Insights into Growth of Listeria Monocytogenes at Refrigeration Temperatures Through Studies of Tn917-Induced Cold-Sensitive Mutants - (Abstract)

Zhu, K., Liu, F., Liu, S., Wilkinson, B.J. 2003. Insights into growth of Listeria monocytogenes at refrigeration temperatures through studies of Tn917-induced cold-sensitive mutants [abstract]. Great Lake Regional Center of Excellence, First Annual Conference, Northwestern University, December 12-14, 2003, Chicago, Illinois. p. 25.