Projects

MICROBIAL CATALYSTS TO PRODUCE FUEL ETHANOL AND VALUE ADDED PRODUCTS Appropriated (D)   Accession Number: 408950

ASSAY DEVELOPMENT FOR A BIOSENSOR TO MINIMIZE ANTIBIOTIC USE IN CORN-TO-ETHANOL MANUFACTURING Reimbursable (R)   Accession Number: 411043

BIOINFORMATICS AND COMPARATIVE GENOMIC ANALYSES F L. BUCHNERI NRRL B-30929 Specific Cooperative Agreement (S)   Accession Number: 411934

Publications

Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri - (Peer Reviewed Journal) - (31-Oct-08)

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2008. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal.

Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks - (Abstract)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting.

Lactobacillus buchneri: A novel biocatalyst for ethanol production - (Abstract)

Liu, S. 2008. Lactobacillus buchneri: A novel biocatalyst for ethanol production [abstract]. MWA Bioenergy Research Forum.

Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology Meeting. 0-030. p. 155.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Controlled antibiotic use during fuel ethanol production - (Abstract)

Bischoff, K.M., Scott, J., Leathers, T.D., Liu, S., Worthington, R. 2008. Controlled antibiotic use during fuel ethanol production [abstract]. National Science Foundation, Partnerships for Innovation Workshop. Poster No. 13.

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.

Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. 3rd International Symposium on Biocatalysis and Biotechnology. p. 49.

Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Peer Reviewed Journal)

Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2008. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products. Journal of Industrial Microbiology and Biotechnology. 35:75-81.

Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.

Monensin inhibits growth of bacterial contaminants from fuel ethanol plants - (Abstract)

Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.

Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 - (Peer Reviewed Journal)

Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154.

A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Abstract)

Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158.

High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.

Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces - (Abstract)

Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces [abstract]. 2007 World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.

Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide - (Abstract)

Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.

Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract)

Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.

DEVELOPING GRAM-POSITIVE ETHANOLOGENS - (Abstract)

Liu, S. 2006. Developing gram-positive ethanologens [abstract]. International Symposium of Biocatalysis and Bioenergy. p. 33.

CHROMOSOMAL INACTIVATION OF THE ALS GENE IN LACTOBACILLUS PLANTARUM STRAIN TF103 TO ELIMINATE UNDESIRED FERMENTATION PRODUCTS - (Abstract)

Liu, S. 2006. Chromosomal inactivation of the als gene in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products [abstract]. Society of Industrial Microbiology. p. 166.

PURIFICATION AND CHARACTERIZATION OF A FAMILY 5 ENDOGLUCANASE FROM A MODERATELY THERMOPHILIC STRAIN OF BACILLUS LICHENIFORMIS - (Peer Reviewed Journal)

Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765.

GENETICALLY ENGINEERED LACTIC ACID BACTERIA FOR THE PRODUCTION OF FUELS AND CHEMICALS - (Abstract)

Liu, S. 2006. Genetically engineered lactic acid bacteria for the production of fuels and chemicals [abstract]. 10th International Symposium on the Genetics of Industrial Microorganisms. Paper #015.

ISOLATION OF XYLOSE-UTILIZING THERMOPHILIC BACTERIA FROM DAIRY MANURE COMPOST - (Abstract)

Bischoff, K.M., Liu, S., Edrington, T.S., Hughes, S.R. 2006. Isolation of xylose-utilizing thermophilic bacteria from dairy manure compost [abstract]. Extremophiles 2006. p. 19. Poster P045.

FUNCTIONAL PROTEOMIC PLASMID-BASED INTEGRATED WORKCELL FOR HIGH-THROUGHPUT TRANSFORMATION OF BL21 DE3 E. COLI FOR EXPRESSION IN VIVO WITH PIROMYCES STRAIN XYLOSE ISOMERASE - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.

A COLD-SENSITIVE LISTERIA MONOCYTOGENES MUTANT HAS A TRANSPOSON INSERTION IN A GENE ENCODING A PUTATIVE MEMBRANE PROTEIN AND SHOWS ALTERED (P)PPGPP LEVELS - (Peer Reviewed Journal)

Liu, S., Bayles, D.O., Mason, T.M., Wilkinson, B.J. 2006. A cold-sensitive Listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein and shows altered (p)ppGpp levels. Applied and Environmental Microbiology. 72(6):3955-3959.

MASS TRANSFORMATION OF PLASMID LIBRARIES OF CDNA OR MUTAGENIZED CLONE SETS INTO YEAST OR BACTERIA USING A FUNCTIONAL PROTEOMIC ROBOTIC WORKCELL - (Abstract)

Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.

CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING GENETICALLY ENGINEERED ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.

CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using Escherichia coli FBR5: membrane and fixed cell reactors [abstract]. American Institute of Chemical Engineers. Paper No. 589g.

A SIMPLE METHOD TO GENERATE CHROMOSOMAL MUTATIONS IN LACTOBACILLUS PLANTARUM STRAIN TF103 TO ELIMINATE UNDESIRED FERMENTATION PRODUCTS - (Peer Reviewed Journal)

Liu, S. 2006. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products. Applied Biochemistry and Biotechnology. 129-132:854-863.

LACTOBACILLUS BREVIS: A POTENTIAL BIOCATALYST FOR LIGNOCELLULOSIC BIOMASS TO ETHANOL - (Abstract)

Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.

CHARACTERIZATION OF CARBOXYMETHYLCELLULASE ACTIVITY FROM GEOBACILLUS STEAROTHERMOPHILUS - (Abstract)

Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38.

METABOLIC ENGINEERING OF A LACTOBACILLUS PLANTARUM DOUBLE LDH KNOCKOUT STRAIN FOR ENHANCED ETHANOL PRODUCTION - (Peer Reviewed Journal)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.

ETHANOL FERMENTATION BY OVEREXPRESSING A GRAM-POSITIVE PDC GENE IN LACTOBACILLUS PLANTARUM STRAIN TF103 - (Abstract)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.

METABOLIC ENGINEERING OF LACTOBACILLUS BREVIS FOR ETHANOL PRODUCTION - (Abstract)

Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.

FUNCTIONAL EXPRESSION OF BACTERIAL ZYMOBACTER PALMAE PYRUVATE DECARBOXYLASE GENE IN LACTIC ACID BACTERIA - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Cotta, M.A. 2005. Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in lactic acid bacteria. Current Microbiology. 50:1-6.

CLONING, EXPRESSION, PURIFICATION, AND ANALYSIS OF MANNITOL DEHYDROGENASE GENE MTLK FROM LACTOBACILLUS BREVIS - (Peer Reviewed Journal)

Liu, S., Saha, B.C., Cotta, M.A. 2005. Cloning, expression, purification, and analysis of mannitol dehydrogenase gene mtlK from Lactobacillus brevis. Applied Biochemistry and Biotechnology. 121-124:391-402.

EXPRESSION ANALYSIS OF PYRUVATE DECARBOXYLASE GENES FROM ZYMOBACTER PALMAE AND SARCINA VENTRICULI IN GRAM-POSITIVE LACTIC ACID BACTERIA - (Abstract)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2004. Expression analysis of pyruvate decarboxylase genes from Zymobacter palmae and Sarcina ventriculi in Gram-positive lactic acid bacteria [abstract]. American Society for Microbiology. Paper No. O-099.

CLONING, EXPRESSION, PURIFICATION, AND ANALYSIS OF MANNITOL DEHYDROGENASE OF MTLD AND MTLK GENES FROM LACTOBACILLUS PLANTARUM AND LACTOBACILLUS BREVIS - (Abstract)

Liu, S., Saha, B.C., Cotta, M.A. 2004. Cloning, expression, purification, and analysis of mannitol dehydrogenase of mtlD and mtlK genes from Lactobacillus plantarum and Lactobacillus brevis [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 2-24.

GENETIC ENGINEERING OF LACTIC ACID BACTERIA TOWARD FERMENTATIVE PRODUCTION OF ETHANOL - (Abstract)

Liu, S., Cotta, M.A. 2004. Genetic engineering of lactic acid bacteria toward fermentative production of ethanol [abstract]. American Society for Microbiology. p. 29.

INSIGHTS INTO GROWTH OF LISTERIA MONOCYTOGENES AT REFRIGERATION TEMPERATURES THROUGH STUDIES OF TN917-INDUCED COLD-SENSITIVE MUTANTS - (Abstract)

Zhu, K., Liu, F., Liu, S., Wilkinson, B.J. 2003. Insights into growth of Listeria monocytogenes at refrigeration temperatures through studies of Tn917-induced cold-sensitive mutants [abstract]. Great Lake Regional Center of Excellence, First Annual Conference, Northwestern University, December 12-14, 2003, Chicago, Illinois. p. 25.