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Agricultural Research Service United States Department of Agriculture
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Timothy D Leathers (Tim)
Bioproducts and Biocatalysis Research
Research Geneticist

Phone: (309) 681-6377
Fax: (309) 681-6040
Room 3104
NTL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH
1815 N UNIVERSITY STREET
PEORIA, IL, 61604-3999

Projects
DEVELOPING BIOCONVERSION PROCESSES FOR HIGH-VALUE CARBOHYDRATE PRODUCTS
Appropriated (D)
  Accession Number: 411114

Publications
Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri - (Peer Reviewed Journal) - (31-Oct-08)
Insoluble glucans from planktonic and biofilm cultures of mutants of Leuconostoc mesenteroides NRRL B-1355 - (Peer Reviewed Journal) - (22-Oct-08)
Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain - (Abstract)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract].
Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks - (Abstract)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting.
Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri - (Abstract)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology Meeting. 0-030. p. 155.
Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)
Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.
Glucans, biofilms, and sugar: A biochemical and practical perspective - (Abstract)
Cote, G.L., Leathers, T.D., Eggleston, G. 2008. Glucans, biofilms, and sugar: A biochemical and practical perspective [abstract]. American Chemical Society National Meeting. CARB 97.
Controlled antibiotic use during fuel ethanol production - (Abstract)
Bischoff, K.M., Scott, J., Leathers, T.D., Liu, S., Worthington, R. 2008. Controlled antibiotic use during fuel ethanol production [abstract]. National Science Foundation, Partnerships for Innovation Workshop. Poster No. 13.
Biofilm formation by exopolysaccharide mutants of Leuconostoc mesenteroides strain NRRL B-1355 - (Peer Reviewed Journal)
Leathers, T.D., Cote, G.L. 2008. Biofilm formation by exopolysaccharide mutants of Leuconostoc mesenteroides strain NRRL B-1355. Applied Microbiology and Biotechnology. 78(6):1025-1031.
Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Peer Reviewed Journal)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2008. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products. Journal of Industrial Microbiology and Biotechnology. 35:75-81.
Endo-B-1,4-xylanase inhibitors in shoots of germinating maize - (Abstract)
Biely, P., Leathers, T.D., Cziszarova, M., Vrsanska, M., Cotta, M.A. 2007. Endo-B-1,4-xylanase inhibitors in shoots of germinating maize [abstract]. Annual Workshop of the Biotechnical Functionalistation of Renewable Polymeric Materials of COST. Abstract #O, p. 1.4.
Genetic Diversity of Tropical Isolates and Color Variants of Aureobasidium pullulans - (Abstract)
Manitchotpisit, P., Leathers, T.D., Li, X., Kurtzman, C.P., Eveleigh, D.E., Prasongsuk, S., Punnapayak, H. 2007. Genetic Diversity of Tropical Isolates and Color Variants of Aureobasidium pullulans [abstract]. Society of Industrial Microbiology Annual Meeting. Abstract S116, p. 113.
Endo-beta-1,4-xylanase inhibitors in leaves and roots of germinated maize - (Peer Reviewed Journal)
Biely, P., Leathers, T.D., Cziszarova, M., Vrsanska, M., Cotta, M.A. 2008. Endo-beta-1,4-xylanase inhibitors in leaves and roots of germinated maize. Journal of Cereal Science. 48(1):27-32.
Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants - (Peer Reviewed Journal)
Bischoff, K.M., Skinner-Nemec, K.A., Leathers, T.D. 2007. Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants. Journal of Industrial Microbiology and Biotechnology. 34(11):739-744.
A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Abstract)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158.
Effect of oil extraction method on the enzymatic digestibility of corn germ arabinoxylan - (Peer Reviewed Journal)
Leathers, T.D., Price, N.P. 2007. Effect of oil extraction method on the enzymatic digestibility of corn germ arabinoxylan. Cereal Chemistry. 84(3):243-245.
Color Variability, Pullulan Production, and Pullulan Degrading Enzyme Activities in Tropical Isolates of Aureobasidium Pullulans - (Abstract)
Manitchotpisit, P., Leathers, T.D., Prasongsuk, S., Eveleigh, D.E., Punnapayak, H. 2006. Color Variability, Pullulan Production, and Pullulan Degrading Enzyme Activities in Tropical Isolates of Aureobasidium Pullulans [abstract]. Abstracts of the 11th Biological Sciences Graduate Congress. p. 45.
BIOFILM FORMATION BY BACTERIAL CONTAMINANTS OF FUEL ETHANOL PRODUCTION - (Peer Reviewed Journal)
Skinner-Nemec, K.A., Nichols, N.N., Leathers, T.D. 2007. Biofilm formation by bacterial contaminants of fuel ethanol production. Biotechnology Letters. 29:379-383.
COLOR VARIABILITY AND PULLULAN PRODUCTION FROM TROPICAL VARIANTS AUREOBASIDIUM PULLULANS - (Abstract)
Manitchotpisit, P., Prasongsuk, S., Eveleigh, D.E., Leathers, T.D., Punnapayak, H. 2006. Color variability and pullulan production from tropical variants aureobasidium pullulans [abstract]. Society of Industrial Microbiology. p. 196.
ANTIMICROBIAL SUSCEPTIBILITY OF LACTOBACILLUS SPECIES ISOLATED FROM FUEL-ETHANOL PLANTS - (Abstract)
Bischoff, K.M., Skinner, K.A., Leathers, T.D. 2006. Antimicrobial susceptibility of lactobacillus species isolated from fuel-ethanol [abstract]. American Society for Microbiology. Poster No. O-038.
SCREENING OF THE SUBSTRATE PROPERTIES OF MICROORGANISMS FOR BIOSENSOR DETECTION OF OLIGOSACCHARIDES - (Abstract)
Kitova, A.E., Reshetilov, A.N., Leathers, T.D., Cotta, M.A. 2006. Screening of the substrate properties of microorganisms for biosensor detection of oligosaccharides [abstract]. Biosensors 2006. Paper No. P40.
PULLULAN PRODUCTION BY TROPICAL ISOLATES OF AUREOBASIDIUM PULLULANS - (Peer Reviewed Journal)
Prasongsuk, S., Berhow, M.A., Dunlap, C.A., Weisleder, D., Leathers, T.D., Eveleigh, D.E., Punnapayak, H. 2007. Pullulan production by tropical isolates of Aureobasidium pullulans. Journal of Industrial Microbiology and Biotechnology. 34(1):55-61.
ENDO-B-1,4-XYLANASE INHIBITORS IN LEAVES AND ROOTS OF GERMINATING MAIZE - (Abstract)
Biely, P., Leathers, T.D., Cotta, M.A. 2005. Endo-b-1,4-xylanase inhibitors in leaves and roots of germinating maize [abstract]. Pacifichem Symposium. Program Number 449.
DEXTRAN - (Book/Chapter)
Leathers, T.D. 2005. Dextran. In: Steinbuchel, A., Marchessault, R.H., editors. Biopolymers for Medical and Pharmaceutical Applications. Vol 1., Humic Substances, Polyisoprenoids, Polyesters, and Polysaccharides, Chapter 15. Wiley-VCH: Weinheim. p. 537-559.
DEXTRAN - (Book/Chapter)
Leathers, T.D. 2005. Dextran. In: Steinbuchel, A., Rhee, S.K., editors. Polysaccharides and Polyamides in the Food Industry. Properties, Production, and Patents. Vol. 1., Polysaccharides, Chapter 6. Wiley-VCH: Weinheim. p. 233-255.
PULLULAN - (Book/Chapter)
Leathers, T.D. 2005. Pullulan. In: Steinbuchel, A., Rhee, S.K., editors. Polysaccharides and Polyamides in the Food Industry. Properties, Production, and Patents. Vol. 1., Polysaccharides, Chapter 11. Wiley-VCH: Weinheim. p. 387-421.
COPRODUCTION OF SCHIZOPHYLLAN AND ARABINOXYLAN FROM CORN FIBER - (Peer Reviewed Journal)
Leathers, T.D., Nunnally, M.S., Price, N.P. 2006. Coproduction of schizophyllan and arabinoxylan from corn fiber. Biotechnology Letters. 28(9):623-626.
PRODUCTION OF THE BIOACTIVE BETA-GLUCAN SCHIZOPHYLLAN FROM CORN FIBER - (Abstract)
Leathers, T.D., Nunnally, M.S., Price, N.P. 2005. Production of the bioactive beta-glucan, schizophyllan, from corn fiber [abstract]. Society of Industrial Microbiology. p. 95.
PULLULAN PRODUCTION BY TROPICAL ISOLATES OF AUREOBASIDIUM PULLULANS - (Abstract)
Prasongsuk, S., Leathers, T.D., Eveleigh, D.E., Punnapayak, H. 2005. Pullulan production by tropical isolates of aureobasidium pullulans [abstract]. Society of Industrial Microbiology. p. 94.
ANTIMICROBIAL SUSCEPTIBILITY OF BACTERIAL CONTAMINANTS FROM A WET-MILL ETHANOL PLANT - (Abstract)
Bischoff, K.M., Skinner-Nemec, K., Leathers, T.D., Hughes, S.R. 2005. Antimicrobial susceptibility of bacterial contaminants from a wet-mill ethanol plant [abstract]. Society for Industrial Microbiology. Poster P42.
A METHOD FOR SURVEYING AND CLASSIFYING LEUCONOSTOC SPP. GLUCANSUCRASES ACCORDING TO STRAIN-DEPENDENT ACCEPTOR PRODUCT PATTERNS - (Peer Reviewed Journal)
Cote, G.L., Leathers, T.D. 2005. A method for surveying and classifying Leuconostoc spp. glucansucrases according to strain-dependent acceptor product patterns. Journal of Industrial Microbiology and Biotechnology. 32(2):53-60.
BIOFILM FORMATION BY BACTERIAL CONTAMINANTS OF FUEL ETHANOL PRODUCTION - (Proceedings/Symposium)
Skinner, K.A., Leathers, T.D. 2004. Biofilm formation by bacterial contaminants of fuel ethanol production. Biofilms 2004: Structure and Activity of Biofilms, October 24-26, 2004. Las Vegas, Nevada. p 210-214.
STRUCTURE AND PROPERTIES OF A NOVEL MODIFIED ALTERNAN - (Abstract)
Leathers, T.D., Nunnally, M.S., Ahlgren, J.A., Cote, G.L. 2004. Structure and properties of a novel modified alternan [abstract]. 36th Great Lakes Regional American Chemical Society Symposium. October 17-20, 2004. Peoria, Illinois. p. 164.
BACTERIAL CONTAMINANTS AND BIOFILM FORMATION IN FUEL ETHANOL PLANTS - (Abstract)
Skinner, K.A., Leathers, T.D. 2004. Bacterial contaminants and biofilm formation in fuel ethanol plants [abstract]. 36th Great Lakes Regional Meeting of American Chemical Society, October 17-20, 2004, Peoria, Illinois. p. 71.
CHARACTERIZATION OF EXOPOLYSACCHARIDES PRODUCED FROM AUREOBASIDIUM PULLULANS - (Abstract)
Prasongsuk, S., Leathers, T.D., Kuhirun, M., Eveleigh, D.E., Punnapayak, H. 2004. Characterization of exopolysaccharides produced from Aureobasidium pullulans [abstract]. Society of Industrial Microbiology. p. 72.
GENETICALLY MODIFIED STRAINS OF FUSARIUM SPOROTRICHIOIDES FOR PRODUCTION OF LYCOPENE AND BETA-CAROTENE. - (Abstract)
Jones, J.D., Hohn, T.M., Leathers, T.D. 2004. Genetically modified strains of Fusarium sporotrichioides for production of lycopene and beta-carotene [abstract]. Society of Industrial Microbiology. p. 91.
BACTERIAL CONTAMINANTS OF FUEL ETHANOL PRODUCTION - (Peer Reviewed Journal)
Skinner, K.A., Leathers, T.D. 2004. Bacterial contaminants of fuel ethanol production. Journal of Industrial Microbiology and Biotechnology. 31:401-408.
UTILIZATION OF CORN GERM RESIDUES - (Proceedings/Symposium)
Leathers, T.D. 2004. Utilization of corn germ residues. In: Proceedings of Corn Utilization Conference. June 7-9, 2004. Indianapolis, Indiana. CDROM.
COMPARISON OF IDENTIFICATION METHODS FOR LACTOBACILLUS - (Proceedings/Symposium)
Skinner, K.A., Leathers, T.D. 2004. Comparison of identification methods for lactobacillus. In: Proceedings of Corn Utilization Technology Conference. June 7-9, 2004, Indianapolis, Indiana. CDROM.
BACTERIAL BIOSENSORS. POTENTIAL APPLICATIONS IN BIOTECHNOLOGY AND ECOLOGICAL MONITORING - (Abstract)
Reshetilov, A.N., Leathers, T.D., Cotta, M.A. 2004. Bacterial biosensors, potential applications in biotechnology and ecological monitoring [abstract]. Biosensors World Congress. Paper No. BS272.
TRANSFORMATION OF ALTERNAN STRAINS OF LEUCONOSTOC BY ELECTROPORATION - (Peer Reviewed Journal)
Leathers, T.D., Jones, J.D., Wyckoff, H.A. 2004. Transformation of alternan-producing strains of Leuconostoc by electroporation. Biotechnology Letters. 26(14):1119-1124.
DEXTRAN - (Book/Chapter)
Leathers, T.D. 2005. Dextran. In Steinbuchel, A., Doi, Y., editors. Biotechnology of Biopolymers, From Synthesis to Patents. Vol. 1. Lignin, Coal, Polyesters and Polysaccharides, Chapter 20, Wiley-VCH, Weinheim. p. 575-597.
ENZYME SACCHARIFICATION OF DEFATTED CORN GERM - (Peer Reviewed Journal)
Leathers, T.D. 2004. Enzyme saccharification of defatted corn germ. Biotechnology Letters. 26:203-207.
APPLICATION OF MALS IN THE CHARACTERIZATION OF A NOVEL POLYSACCHARIDE, MODIFIED ALTERNAN - (Abstract)
Ahlgren, J.A., Leathers, T.D., Nunnally, M.S., Cote, G.L. 2003. Application of mals in the characterization of a novel polysaccharide, modified alternan [abstract]. International Light Scattering Colloquium. Paper No. 4.
CHARACTERIZATION OF A NOVEL MODIFIED ALTERNAN - (Abstract)
Leathers, T.D., Nunnally, M.S., Ahlgren, J.A., Cote, G.L. 2003. Characterization of a novel modified alternan [abstract]. Society of Industrial Microbiology. p. 100.
COMPARISON OF BACTERIA POPULATIONS AMONG FUEL ETHANOL PLANTS - (Abstract)
Skinner, K.A., Leathers, T.D. 2003. Comparison of bacteria popularions among fuel ethanol plants [abstract]. Society of Industrial Microbiology. p. 104.
BIOTECHNOLOGICAL PRODUCTION AND APPLICATIONS OF PULLULAN - (Review Article)
Leathers, T.D. 2003. Biotechnological production and applications of pullulan. Applied Microbiology and Biotechnology. 62:468-473.
CHARACTERIZATION OF A NOVEL MODIFIED ALTERNAN - (Peer Reviewed Journal)
Leathers, T.D., Nunnally, M.S., Ahlgren, J.A., Cote, G.L. 2003. Characterization of a novel modified alternan. Carbohydrate Polymers. 54:107-113.
NOVEL PENICILLIUM ISOLATES FOR MODIFYING ALTERNAN - (Patent Application)
Leathers, T.D., Nunnally, M.S., Cote, G.L. 2003. Novel penicillium isolates for modifying alternan. Patent Cooperation Treaty (PCT) International Publication No. WO 03/010177.
NOVEL SYSTEM FOR THE SEQUENTIAL, DIRECTIONAL CLONING OF MULTIPLE DNA SEQUENCES AND ITS USE IN METABOLIC ENGINEERING - (Abstract)
Jones, J.D., Hohn, T.M., Leathers, T.D. 2002. Novel system for the sequential, directional cloning of multiple DNA sequences and its use in metabolic engineering [abstract]. Metabolic Profiling: Pathways in Discovery. Poster #10.
BIOCONVERSIONS OF AGRICULTURAL RESIDUES TO VALUE-ADDED COPRODUCTS USING YEASTLIKE FUNGI - (Review Article)
Leathers, T.D. 2003. Bioconversions of maize residues to value-added coproducts using yeastlike fungi. Federation of European Microbiological Societies Yeast Research. 3:133-140.
CORN FIBER HYDROLYSIS BY THERMOBIFIDA FUSCA EXTRACELLULAR ENZYMES - (Peer Reviewed Journal)
Irwin, D., Leathers, T.D., Greene, R.V., Wilson, D.B. 2003. Corn fiber hydrolysis by thermobifida fusca extracellular enzymes. Applied Microbiology and Biotechnology. 61:352-258.

   
 
Last Modified: 11/05/2008
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