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Wayne Hunter,Ph.D.
Research Entomologist
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U. S. Horticultural Research Laboratory
2001 South Rock Road
Fort Pierce, FL 34945
Telephone: (772) 462-5898
Fax: (772) 462-5986
Email:
Wayne.Hunter@ars.usda.gov
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Adult Glassy-winged Sharpshooter Leafhopper, GWSS, Homalodisca vitripennis
Germar,. Vector of Pierce's disease of grapes, a xylem-limited bacterium,
Xylella fastidiosa. Adults are about 1/2 inch, (12 mm) long.
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Current Research Projects:
Dr. Hunter, Lead Scientist conducting research on the spread of plant
diseases by insects.The main focus of research is on the glassy-winged sharpshooter, GWSS,
and other leafhoppers which spread the plant infecting bacteria,
Xylella fastidiosa,at the U.S. Horticultural Research Lab,
Fort Pierce, Florida.
Dr. Hunter works to provide solutions to insect problems under USDA-ARS
National Program 304, Crop Protection and Quarantine.
Dr. Hunter’s lab is elucidating GWSS biology using a genomics approach
and has produced and published ~25,000 genetic sequences isolated from
the GWSS.
These can be found at the National Center Biotechnology and Information,
NCBI, expressed sequence tag, EST database for access by the research and
education community.
Continuing examination of GWSS proteins have led to a deeper understanding
of leafhopper feeding, development, and pathogen interactions.
He is the founder of the ‘Southeastern Xylella Sharpshooter Initiative’
aimed at increasing interactions among researchers on Pierce’s disease
and leafhoppers.
Dr. Hunter is also examining the use of insect viruses as a possible management
tools to reduce insect populations, such as GWSS.
He has discovered several new insect viruses isolated from aphids, leafhoppers,
psyllids, and fire ants.
Dr. Hunter also conducts research on exotic insect pests.
He has produced over 120,000 sequences from many different insect species:
Aphids, (
Toxoptera citricida, Acyrthosiphon pisum, Aphis gossypii); Leafhoppers, (
Homalodisca coagulata, Oncometopia nigricans); Asian Citrus Psyllid, (
Diaphorina citri); Pink hibiscus mealybug, (
Maconellicoccus hirsutus); citrus weevil, (
Diaprepes abbreviatus); Asian longhorn beetle, (
Anoplophora glabripennis); and parasitoids (
Lysiphlebus testaceipes, Nasonia).
He was awarded ‘Entomologist of the Year 2005’ from the Florida Entomological
Society for, “Internationally recognized contributions to insect genomics”.
He is a member of the International Aphid Genomics working group, working
with researchers from the USA, France, Spain, Japan, and Australia.
The aphid genome effort has resulted in the production of two pea aphid
microarrays which are being used to increase our understanding of gene
expression in aphid biology.
Dr. Hunter is also a member of the Genome Steering committee for the National
Parasitoid Genome Project,
Nasonia and the International Whitefly Genome Initiative.
He also organizes the ‘International Insect Genomics Workshop at
the International Plant & Animal Genome conference.
Dr. Hunter is a member of the ‘International Grape Genome Program’, and
a co-developer of the ‘North American Grape Genome Database’, which is
hosted at FloridaA&MUniversity.
North American grape varieties have greater resistance to grape diseases
and insect pests.
Design of genetic markers linked to resistance traits are being used in
a maker-assisted selection program to produce new grape varieties with
resistance to important grape diseases, such as Pierce's Disease, and fungal
pathogens.
Due to the economic importance of the Asian citrus psyllid, Diaphorina
citri, as a vector of bacteria associated with the disease Huanglongbing,
HLB in citrus, several new genomics efforts have been started to address
many of the unknowns surrounding the cause and spread of HLB.
 
International Asian Citrus Psyllid Genome Consortium
Hunter, Wayne B. (Lead) USA
National Program Staff:
Dr. Kevin Hackett, NPL, ARS, Beltsville, MD (Insects)
Dr. Gail Wisler, NPL, ARS, Beltsville, MD (Plant Disease)
Steering Committee:
Dr. Susan Brown, KSU, Arthropod Genomics Consortium, KS
Dr. Stephen Richardson, Human Genome Sequencing Center, Baylor Univ.,
TX
Dr. Jay Evans, USDA, ARS, Honey bee genomics, Beltsville, MD
Working Group Members:contact
Wayne.Hunter@ars.usda.gov
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Project ID:
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29473
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Project Status:
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Accepted
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Project Type:
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Single Species Project
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Lead
Contact's Name:
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Wayne Hunter,
Wayne.hunter@ars.usda.gov
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Submitting Organization:
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USDA, ARS, U.S.Hort. Res. Lab, Ft. Pierce, FL
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Contact's Name:
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Robert Shatters, Jr.,
Robert.shatters@ars.usda.gov
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Contact's Name:
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David Hall,
David.hall@ars.usda.gov
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Submitting Organization:
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USDA, ARS, U.S.Hort. Res. Lab, Ft. Pierce, FL
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Locus Tag Prefix:
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DIACI
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Source of DNA:
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W. Hunter, Colonies, U.S.Horticultural Res. Lab
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2001 South Rock Road, Ft. Pierce, FL, USA
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The actual causes of Huanglongbing, HLB, (known as ‘Citrus Yellow Dragon
Disease’ or ‘Citrus Greening’) in citrus is currently unknown. While the
bacteria, Candidatus Liberibacter asiaticus, CLa, has been shown to be
associated with the occurrence of HLB in citrus, there is a lack of data
on all the microorganisms associated with the ‘cause’ of HLB. There is
growing agreement within the scientific community that the cause may be
due to the interaction of several different pathogens.
To resolve this issue a genomics approach was proposed and developed,
incorporating a Metagenomics approach to identify all possible micro organisms
associated with HLB. By using high-resolution community-wide genomic information,
we can describe the composition, function and emergent properties of the
integrated microbial community within the citrus tree and within the psyllid
vector more accurately.
While a genomics effort is being applied to the Asian citrus psyllid,
AsCP, to provide insight into psyllid biology, the Metagenomic project
provides a deeper understanding of the micro-world within the psyllid.
The DNA from psyllids or from the phloem of citrus trees contains a community
of organisms that may include a wide range of organisms from bacterial
to viral species at different levels of diversity and abundance. Since
many microbes resist being cultured, culture-independent methods such as
genomics is often the only way for identifying and enumerating microbes
in samples like citrus phloem and the psyllid.>
Therefore, an International Asian Citrus Psyllid Genomics consortium was
organized, bringing together researchers working on insects, bacteria,
psyllid vectors, plant disease, Genomics, Bioinformatics, Pathology, Microbiology
and others. The main objectives will be to identify the causal agents of
HLB, and then to apply these answers to the development of innovative management
strategies against this devastating citrus disease.
International Diaphorina citri Wolbachia Genome Consortium
David Hall, RL, USHRL, ARS, Ft. Pierce, FL
Yong Ping Duan, USHRL, ARS, Ft. Pierce, FL
Wayne Hunter, USHRL, ARS, Ft. Pierce, FL
Robert Shatters, USHRL, ARS, Ft. Pierce, FL
Dave Schneider, ARS, Cornell Univ., NY
Working Group forming:
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Project ID:
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29451
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Project Status:
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Accepted
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Project Type:
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Single Species Project
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Contact's Name:
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Wayne Hunter,
Wayne.hunter@ars.usda.gov
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Contact's Name:
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Yong Ping Duan,
YongPing.Duan@ars.usda.gov
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Contact's Name:
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Robert Shatters, Jr.,
Robert.shatters@ars.usda.gov
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Contact's Name:
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David Hall,
David.hall@ars.usda.gov
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Submitting Organization:
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USDA, ARS, U.S.Hort. Res. Lab, Ft. Pierce, FL
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URL:
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http://www.ars.usda.gov/main/site_main.htm?modecode=66-18-00-00
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Consortium Name:
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International
Diaphorina citri
WolbachiaGenome Consortium
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Organism Name:
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Wolbachia endosymbiont of
Diaphorina citri
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Strain/isolate/breed:
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FL
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Locus Tag Prefix:
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WDIAC
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Source of DNA:
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Yong Ping Duan, USDA, ARS, U.S.Hort. Res. Lab,
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2001 South Rock Road, Ft. Pierce, FL, USA
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General Information on GWSS:
 
Scientific name of the glassy-winged sharpshooter, GWSS: Homalodisca vitripennis
Germar, is the main vector of the plant infecting pathogenic bacterium, Xylella fastidiosa.
Xylellabacterium, causes Pierce’s Disease of grapes, and many other 'Scorch-like’
plant diseases.
Since the GWSS can fly long distances, and feeds from a wide range of
plants it can spread these bacteria far and wide through many different
crops. Grapes, almonds, peaches and other tree crops especially are
heavy hit by these pathogens.
Examination of the developmental genes within the eggs, embryos, and nymphs
is aiding efforts to develop environmentally safe and efficient long-term
management strategies to disrupt insect development and reduce the spread
of Xylellacaused diseases.
How damage is caused:
GWSS is a piercing-sucking feeder that extracts fluid from the xylem tissues
of plants. This feeding spreads the bacterial diseases to other host plants.
The pest is highly mobile, flying long distances, and feeds on many different
plants, thus spreading the disease to many plants in a single day. The
GWSS has over 100 different host plants on which it feeds. The
bacterium Xylella fastidiosa is spread during GWSS feeding. The interactions
between bacterium and plant induces the symptoms similar to
drought, wherin the leaves and stems dry out, thus infected plants
lose their vigor and gradually die.
Other diseases vectored by GWSS (strains of Xylella fastidiosa):
Pierce's disease (grapes)
Leaf scorch (almonds)
Alfalfa dwarf (alfalfa)
Oleander scorch (oleander)
Phony peach disease (peaches)
Plum leaf scald (plums)
Citrus variegated chlorosis (citrus)
Many other leafhopper species, such as the black-winged sharpshooter, Oncometopia nigricanscan also transmit and spread Xylellacaused plant diseases.
Intensive comparisons of the genetics between different leafhopper species
is providing insights into how the pathogen interacts with the insect vectors,
and adding to our understanding of pathogen acquisition and transmission.
Discovering Insect Viral Pathogens:
Newly discovered insect viruses, specific to sharpshooters, such as HoCV-1,
which reduces GWSS survival, along with discovery of viruses in other insects,
like fire-ants, SINV-1, and in many other insect pests demonstrates that
there are a lot of unexploited insect pathogens yet to be discovered which
may serve to reduce insect pests.
Iridovirus infection in GWSS fatbody tissues (pictured above) within the
head of adults that were injected with virus into the lower abdomen.
Examination of virus infection shows that IIV6 infects fatbody, muscle,
and nerve tissues of GWSS.
Mortality ranged from 20-30%, and egg production was reduced.
Insect infecting viruses may provide a means to reduce insect pests in
an environmentally friendly manner.
The main obstacle is the development of a method for mass production of
virus in a cost efficient manner.
Modern technological breakthroughs may soon make this approach a feasible,
cost effective means of insect management.
New discoveries of insect infecting viruses, which are highly specific
to the insects which they infect may provide new means to manage insect
pests.
In the Diaprepes root weevil, DRW, we have shown that infected males can
transfer virus to non-infected females, which subsequently become infected
and can then transfer the virus back into males during mating.
In insects that mate multiple times using a virus suppression holds great
promise as more individuals become infected and either die or become sterilized.
Exotic Insect Pests:
 
TOXOPTERA citricida,the Brown Citrus Aphid, BrCA, is the main vector of Citrus Tristeza virus,
CTV, in citrus growing regions.
The aphids produce two morphologically distinct adults one and one with
wings (Alate), and without wings (Apterous).
In the winged stage the aphids disperse to nearby host trees and begin
to feed thus transferring the virus and infecting more citrus trees.
Genomic analyses is permitting a closer examination of the genes and proteins
playing critical roles in virus acquisition and transmission, as well as
exposing the intimate interactions between insect and host plants.
Dr. Hunter's Most Recent Publication:
W.B. Hunter, S.E. Dowd, D.S. Katsar, R.G. Shatters Jr., C.L. McKenzie and D.G. Hall.
Psyllid Biology:
Expressed Genes in Adult Asian Citrus Psyllids, Diaphorina citri Kuwayama. The Open Entomology Journal, 2009, 3, 18-29.
Dr. Hunter's Publication List (2002-2009)
Dr. Hunter's Publications (1995 -2002):
Hunter, W.B., Sinisterra, X.H., McKenzie, C.L., and Shatters, Jr., R.G. 2002. Iridovirus
infection and vertical transmission in citrus aphids. Proceedings of the
Florida Horticultural Society 114:70-72.
McKenzie, CL., Lapointe, SL., Hunter, WB., and GJ. Puterka. 2002. Efficacy of Surround for control of Asian citrus
psyllid on citrus. Arthropod Management Tests 27: (Report).
McKenzie, C.L., W.B. Hunter, S.L. Lapointe, and P.M. Dang. 2002. Iridovirus: potential entomopathogenic
biocontrol agent for whitefly. Silverleaf Whitefly National Research, Action,
and Technology Transfer Plan: Fourth Annual Review of the Second 5-Year
Plan and Final Report for 1992-2002. USDA-ARS 2002-01: 162.
McKenzie, CL., Hunter, WB., Lapointe, SL., and Dang, P. 2002. Iridovirus infection and topical application
to whitefly. Proceedings of the Florida State Horticultural Society 114:64-67.
Hunter, W.B., and Polston, J.E. 2001.
Development of a continuous whitefly cell line [Homoptera: Aleyrodidae:
Bemisia tabaci (Gennadius)] for the study of Begomovirus.Journal of Invertebrate Pathology 77: 33-36.
Funk, C J., Hunter, W.B., and Achor, DS. 2001.
Replication of insect iridescent virus 6 in a whitefly cell line. Journal of Invertebrate Pathology 77: 144-146.
Mayer, Richard T., Wayne B. Hunter, Stephen L. Lapointe, C. L. McKenzie, R. Shatters, J. Shapiro, Hamed
Doostdar and A. A. Weathersbee III. 2001. Diaprepes Root Weevil: Recent
Advances at the U.S. Horticultural Research Laboratory, Fort Pierce. Citrus
& Vegetable Magazine, Part 2, Vol. 65, No. 12, August 2001, pp. 8-13.
Mayer, Richard T., Wayne B. Hunter, Stephen L. Lapointe, C. L. McKenzie, R. Shatters, J. Shapiro, Hamed
Doostdar and A. A. Weathersbee III. 2001. Diaprepes Root Weevil: Recent
Advances at the U.S. Horticultural Research Laboratory, Fort Pierce. Citrus
& Vegetable Magazine. Part 1, Vol. 65, No. 11, July 2001, pp. 9-12.
Hunter, W.B., C.P. Patte, X.H. Sinisterra, D.S. Achor, C.J. Funk and J.E. Polston.
2001.
Discovering new insect viruses: Whitefly iridovirus (Homoptera: Aleyrodidae:
Bemisia tabaci). Journal of Invertebrate Pathology 78: 220-225.
Lapointe, S. L., W. B. Hunter, and C. J. Funk(2000).
Infection of the Diaprepes root weevil, Diaprepes abbreviatus (Coleoptera:
Curculionidae), by an Iridovirus.
Hunter, W.B.and H.T. Hsu. 1999.
Synthetic diets and piercing-sucking insect research. Intl. J. Ecol. Environ. Science 25: 259-266.
Tang, Y.Q., S.L. Lapointe, L.C. Brown, and W. B. Hunter. 1999. Effects of host plant and temperature on the biology of Toxoptera
citricida (Homoptera: Aphididae). Environ. Entomol. 28(5): 895-900.
Hunter, W. B., E. Hiebert, S. E. Webb, J. H. Tsai & J. E. Polston. 1997.
Location of geminivirus in the whitefly, Bemisia tabaci (Homoptera: Aleyrodidae). Plant Dis. 82: 1147-1151.
Hunter, W. B., E. Hiebert, S. Webb, J. E. Polston, and J. H. Tsai. 1996 Precibarial
and Cibarial Chemosensilla in the Whitefly, Bemisia Tabaci(Gennadius),
(Homoptera: Aleyrodidae) Int. J Insect Morphol. and Embryol. 25(3): 295-304.
Hunter, W. B.& H. T. Hsu. 1996.
Formulation of an insect medium for thrips monolayer cell cultures (Thysanoptera:
Thripidae: Frankliniella occidentalis). J. Invert. Pathol. 67:125-128.
Hunter, W. B.& H. T. Hsu. 1996. Inoculation of thrips cell mono-layers with a tospovirus.
pp. 359-363. In International Symposium on Tospoviruses and Thrips of Floral
and Vegetable Crops. George Kuo (ed). Acta Horticulturae, No. 431. ISHS.
Asian Vegetable Research & Development Center, AVRDC.
Hunter, W. B.& H. T. Hsu. 1995.
Establishing Thrips Cell Cultures to Study Tospoviruses.pp. 163-166. In Thrips Biology and Management, Bruce L. Parker, Margaret
Skinner, and Trevor Lewis (eds). Plenum Press, New York.
Hunter, W. B., H. T. Hsu & R. Lawson. 1995.
A novel method for acquisition of Tospoviruses by thrips. Phytopathology 85(4): 480-483. (Public Database).
Dr. Hunter's Project List
Published EST Gene Sequences:
Hunter, W.B, Hunnicutt, L.E. 2007. Dataset of 162 Proteins expressed in the pink
hibiscus mealybug,
Maconellicoccus hirsutus(Hemiptera: Pseudococcidae). Accession no. EF070444-EF070605. National
Center for Biotechnology Information, NCBI. (Public Database).
Hunter, W.B., Dang, P.M., Nguyen, R. 2007. Genes expressed in field-caught pink hibiscus
mealybugs,
Maconellicoccus hirsutusNCBI.(Public Information, Biotechnology for Center National EH211307-EH218975.
nos.: Acc. sequences, mRNA 7,669 Pseudococcidae). (Hemiptera: (Green)>
Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2007. Gene expression
in adult blue-green sharpshooters,
Graphocephala atropunctata(Signoret) (Hemiptera: Cicadellidae)6,481 mRNA sequences. Acc. nos.: EH655849-EH662332. National Center for
Biotechnology Information, NCBI. (Public Database).
Mozoruk, J, Hunnicutt, LE, Cave, RD,
Hunter, W.B.
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Bausher, MG. 2006. Profiling transcriptional changes in
Citrus sinensis(L.) Osbeck challenged by herbivory from the xylem-feeding leafhopper
Homalodisca coagulata(Say) by cDNA macroarray analysis. Plant Science 170:1068-1080.
Hunter,W.B.,Hunnicutt,L.E., Hall, D.G. 2006. Ribosomal proteins of the Asian citrus
psyllid,
Diaphorina citri. 88 Proteins, Acc. nos: DQ765535-DQ673441. National Center for Biotechnology
Information, NCBI. http://www4.ncbi.nlm.nih.gov/. (Public Database).
Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2006. Proteins expressed
in the Blue-green sharpshooter,
Graphocephala atropunctata(Hemiptera: Cicadellidae). 44 Proteins, Acc. nos.: DQ445499-DQ445542.
National Center for Biotechnology Information, NCBI. (Public Database).
Weathersbee, A.A., Panchal,T.D., Hunter,W.B.and Dang,P.M. 2006. Expressed Genes from
Lysiphlebus testaceipes(Hymenoptera: Braconidae). 7,762 mRNA sequences, Acc. nos.: EH009595-EH017357.
National Center for Biotechnology Information, NCBI. (Public Database).
Hunter W.B.,Dang PM, Puterka G, Shatters RG, Jr, McKenzie CL, Sinisterra XH. 2005.
5th Instar Glassy-winged Sharpshooter Homalodisca coagulata, (Hemiptera: Cicadellidae) Accession numbers- DN195890-DN203579, 3,921
mRNA sequences. National Center for Biotechnology Information, NCBI.
http://www.ncbi.nlm.nih.gov/
Hunter W.B.,Mizell RF, III, Tipping C, Dang PM, Hunnicutt LE. 2005. Adult sharpshooter
leafhopper
Oncometopia nigricans, (Hemiptera: Cicadellidae), cDNA clones, Acc.Nos.-DR755012-DR759538.
4,527 mRNA sequences. NCBI.
http://www.ncbi.nlm.nih.gov/
Weathersbee, AA, III, Hunter,W.B., Panchal, TD, Dang, PM. 2005.
Lysiphlebus testaceipes,(Hymenoptera: Braconidae), Acc. Nos.- DQ062217- DQ062237 and AY863030-AY961584.
Set of 123 putative full cDNA sequences with identities.
http://www.ncbi.nlm.nih.gov/
Hunter W.B., Dang PM, Lee L. 2005. Adult alate
Aphis gossypii, (Hemiptera: Aphididae), Acc. No.-DR389179-DR397419.
8,241 mRNA sequences. NCBI.
http://www.ncbi.nlm.nih.gov/
Hunter W.B., Smith M. 2005. 5th-instar
Anoplophora glabripennislarva (Coleoptera: Cerambicidae) cDNA clones, Acc. Nos.-DR108748-DR109303,
Set of 556 mRNA sequences.
Plus two full-length
cDNA, DQ067276 & DQ067275. NCBI.
http://www.ncbi.nlm.nih.gov/
Hunter W.B.
, Dang, PM, McKenzie, CL. 2005
Diaphorina citriKuwayama (Hemiptera: Psyllidae)
Diaphorina citri, Acc. nos.-
DN465721-DN470410,
4,686 mRNA sequences
http://www.ncbi.nlm.nih.gov/
Hunter W.B., McKenzie CL, Shatters RG. Weathersbee AA. Hall DG. Gene expression in
field collected Asian citrus psylla,
Diaphorina citriKuwayama (Hemiptera: Psyllidae) 1,220 EST’s, Acc. Nos. DN202325- DN201110.
NCBI.
http://www.ncbi.nlm.nih.gov/
Werren, J, Hunter, W., Dang, P. 2005.
Nasonia, Produced cDNA expression libraries, (Sequencing done by TIGR, 17,000
sequences,TIGR database).
Hunter W.B. and Dang, PM. 2005. Gene expression in filed collected Asian citrus psyllid,
Diaphorina citri, (Hemiptera: Psyllidae). National Center for Biotechnology Information,
NCBI.
http://www.ncbi.nlm.nih.gov/
Hunter WB., Dang PM, Cabrera I. Powell CA. 2005. Gene expression in field collected
Citrus Root Weevil, Diaprepes abbreviatusL. (DRW) (Coleoptera: Curculionidae). Acc. Nos.-DN199437-DN201109. 1,674
mRNA sequences. NCBI. (Public Database).
http://www.ncbi.nlm.nih.gov/
Hunter, WB.Dang,P.M., Chaparro,J.X., Lu,J., Huang,H. and Leong,S. 2004. Genes expressed
in
Vitis shuttleworthii. Grapes. Accession no. CN603662-CN605482, CV092198- CV100862, CF568781-CF568998.
NationalCenterfor Biotechnology Information, NCBI.
http://www.ncbi.nlm.nih.gov/
(Public Database).
Hunter, W.B.2003. Published 4,320 electronic pages: Adult Alate Brown Citrus Aphid
Toxoptera citricidacDNA clones.
U.S.Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL34945, USA.
Accession no. CB832665-CD452353. NationalCenterfor Biotechnology Information, NCBI.
http://www.ncbi.nlm.nih.gov/
(Public Database).
Hunter, WB.2003. Published 427 electronic pages. Data Set: Glassy-winged Sharpshooter,
Expressed Sequence Tags, ESTs, from Adult
Homalodisca coagulata(Say) (Hemiptera: Cicadellidae). GenBank Accession Numbers CF194966 through
CF195393.
NationalCenterfor Biotechnology Information, NCBI.
http://www.ncbi.nlm.nih.gov/
(Public Database).
Hunter, W.B.and Dang, P.M. 2003. Published 970 electronic pages: Gene expression in
Acyrthosiphon pisum(Hemiptera: Aphididae).
U.S.Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL34945, USA.
Accession no. CF587442-CF588411.
NationalCenterfor Biotechnology Information, NCBI.
http://www.ncbi.nlm.nih.gov/
(Public Database).
Bausher, M., McKendree, W., Dang, P., Chaparro, J., Shatters, R., Hunter, W.B., Niedz, R 2002. Published 2,306 electronic pages of “EST high quality
sequences from a
Citrus sinensis(sweet orange) whole seedling cDNA library” to NCBI EST database.
http://www.ncbi.nlm.nih.gov/ (Public Database).
Datasets Submitted NCBI:
Hunter, W.B., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006. Salivary
gland gene expression in glassy-winged sharpshooter, Homalodisca vitripennis
(syn. H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 sequences.
Accession nos.: EG372472-EG368539. National Center for Biotechnology Information,
NCBI. (Public Database).
Hunter, W.B., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006. Midgut gene
expression in glassy-winged sharpshooter, Homalodisca vitripennis (syn.
H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 sequences. Accession
# EG368538-EG365118. National Center for Biotechnology Information, NCBI.
(Public Database).
Desjardins, C.A., Oliveira, D.C.S.G., Edwards, R.M., Dang, P.M., Lee,
D., Colbourne, J.K., Tettelin, H., Hunter, W.B., Werren, J.H. 2007. Nasonia expression libraries from larvae, pupae,
and adults. 37,360 sequences published. Acc. no.: ES6512267-ES613911. National
Center for Biotechnology Information, NCBI. (Public Database).
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