Projects

TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES: THE ROLE OF GENETICS, STRAIN VARIATION, AND ENVIRONMENTAL CONTAMINATION IN DISEASE CONTROL Appropriated (D)   Accession Number: 411463

STRAIN TYPING OF CHRONIC WASTING DISEASE (CWD) AND SCRAPIE BY INTRACEREBRAL INOCULATION INTO TRANSGENIC AND INBRED MOUSE LINES Specific Cooperative Agreement (S)   Accession Number: 411895

DISSEMINATION OF ABNORMAL PRION PROTEIN IN THE NEURAL AND EXTRANEURAL TISSUES OF WILD ROCKY MOUNTAIN ELK Specific Cooperative Agreement (S)   Accession Number: 413374

THE ROLE OF THE PLACENTA IN TRANSMISSION OF EXPERIMENTAL BSE FROM SHEEP TO SHEEP Specific Cooperative Agreement (S)   Accession Number: 408862

TRANSGENIC ANALYSIS OF CHRONIC WASTING DISEASE STRAINS Specific Cooperative Agreement (S)   Accession Number: 409983

Publications

Ovine progressive pneumonia provirus levels are un affected by the prion 171R allele in an Idaho sheep flock - (Peer Reviewed Journal) - (22-Sep-08)

Antemortem detection of PrP-CWD in preclinical, ranch-raised Rocky Mountain elk (Cervus elaphus nelsoni) by biopsy of the rectal mucosa - (Peer Reviewed Journal) - (10-Aug-08)

Small ruminant lentivirus enchances PrP-Sc accumulation in cultured sheep microglial cells - (Peer Reviewed Journal) - (31-Jul-08)

Scrapie resistance in ARQ sheep - (Peer Reviewed Journal)

Laegreid, W.W., Clawson, M.L., Heaton, M.P., Green, B.T., Orourke, K.I., Knowles Jr, D.P. 2008. Scrapie resistance in ARQ sheep. Journal of Virology. 82(20):10318-10320. Available: DOI:10.1128/JVI.00710-08.

Prion gene (PRNP) haplotype variation in United States goat breeds - (Peer Reviewed Journal)

White, S.N., Hoesing, L.M., O'Rourke, K.I., Waldron, D., Rowe, J., Alverson, J. 2008. Prion gene (PRNP) haplotype variation in United States goat breeds. Genetics Selection Evolution. 40(5):553-561.

Chronic wasting disease in a Wisconsin white-tailed deer farm - (Peer Reviewed Journal)

Keane, D.P., Barr, D.J., Bochsler, P.N., Hall, S.M., Gidlewski, T.E., O'Rourke, K.I., Spraker, T.R., Samuel, M.D. 2008. Chronic wasting disease in a Wisconsin white-tailed deer farm. Journal of Veterinary Diagnostic Investigation. 20(5):698-703.

Myenteric neurons of the ileum that express somatostatin are a target of prion neuroinvasion in an alimentary model of sheep scrapie - (Peer Reviewed Journal)

Schneider, D.A., Yan, H., Fry, L.M., Alverson, J., White, S.N., Orourke, K.I. 2008. Myenteric neurons of the ileum that express somatostatin are a target of prion neuroinvasion in an alimentary model of sheep scrapie. Acta Neuropathologica. 115(6):651-661.

Effects of nutrition and genotype on prion protein (PrP-c) gene expression and localization in the fetal and maternal sheep placenta. - (Peer Reviewed Journal)

Evoniuk, J., Johnson, M., Borowicz, P., Caton, J.S., Vonnahme, K., Reynolds, J., Taylor, J.B., Stoltenow, C., Orourke, K.I., Redmer, D. 2008. Effects of Nutrition and Genotype on Prion Protein (PrPC) Gene Expression in the Fetal and Maternal Sheep Placenta. Placenta. 29(5):422-428.

Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route - (Peer Reviewed Journal)

Hamir, A.N., Richt, J., Miller, J.M., Kunkle, R.A., Hall, S., Nicholson, E.M., Orourke, K.I., Greenlee, J.J., Williams, E.S. 2008. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route. Veterinary Pathology. 45(3):297-306.

A species barrier limits transmission of chronic wasting disease to mink (Mustela vison) - (Peer Reviewed Journal)

Harrington, R.D., Baszler, T.V., Orourke, K.I., Schneider, D.A., Spraker, T.R., Liggitt, H.D., Knowles Jr, D.P. 2008. A species barrier limits transmission of chronic wasting disease to mink (Mustela vison). Journal of General Virology. 89(4):1086-1096.

A collaborative Canadian-United Kingdom evaluation of an immunohistochemistry protocol to diagnose bovine spongiform encephalopathy - (Peer Reviewed Journal)

Manning, L., Orourke, K.I., Knowles Jr, D.P., Marsh, S., Spencer, Y.I., Moffat, E., Wells, G.A., Czub, S. 2008. A collaborative Canadian-United Kingdom evaluation of an immunohistochemistry protocol to diagnose bovine spongiform encephalopathy. Journal of Veterinary Diagnostic Investigation. 20(4):113-117.

Scrapie resistance in ARQ sheep - (Abstract)

Laegreid, W.W., Clawson, M.L., Heaton, M.P., Green, B.T., Orourke, K.I., Knowles Jr, D.P. 2008. Scrapie resistance in ARQ sheep (abstract). Plant and Animal Genome XVI Conference. Poster No. P550.

Assessment of direct and indirect transmission of CWD from three cervid species to humans - (Abstract)

Zheng, M., Qing, L., Huang, S., Chen, F., Wang, M., Wang, L., Miller, M., Hamir, A.N., Richt, J.A., O'Rourke, K., Belay, E.D., Schonberger, L.B., Gambetti, P., Kong, Q. 2007. Assessment of direct and indirect transmission of CWD from three cervid species to humans [abstract]. Prion 2007. Paper No. FC5.8.

Elk with a long incubation prion disease phenotype have a unique PrP-d profile - (Peer Reviewed Journal)

O'Rourke, K.I., Spraker, T.R., Zhuang, D., Greenlee, J.J., Gidlewski, T.E., Hamir, A.N. 2007. Elk with a long incubation prion disease phenotype have a unique PrP-d profile. NeuroReport. 18(18):1935-1938.

Association between genotypes at codon 171 and 136 of the prion protein gene and production traits in market lambs - (Peer Reviewed Journal)

Evoniuk, J.M., Berg, P., Johnson, M.L., Larson, D.M., Maddock, T., Stoltenow, C.L., Schauer, C.S., Orourke, K.I., Redmer, D.A. 2007. Association between genotypes at codon 171 and 136 of the prion protein gene and production traits in market lambs. American Journal of Veterinary Research. 68(10):1073-1078.

Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD - (Abstract)

Spraker, T., Gidleweski, T., Greenlee, J., Keane, D., Hamir, A., Orourke, K. 2007. Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Meeting. p. 47.

Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities - (Government Publication)

Kehrli, Jr., M.E., O'Rourke, K.I., Hamir, A.N., Richt, J.A., Nicholson, E.M., Silva, C.J., Edelman, D., Gay, C.G. 2007. Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities [government white paper]. Beltsville, MD: Interagency Working Group on Prion Science, Subcommittee on Pathobiology and Diagnostics. USDA, Agriculture Research Service. 33 p.

Development of an assay to determine single nucleotide polymorphisms in the prion gene for the genetic diagnosis of relative susceptibility to classical scrapie in sheep - (Peer Reviewed Journal)

Johnson, M.L., Evoniuk, J.M., Stoltenow, C.L., Orourke, K.I., Redmer, D.A. 2007. Development of an assay to determine single nucleotide polymorphisms in the prion gene for the genetic diagnosis of relative susceptibility to classical scrapie in sheep. Journal of Veterinary Diagnostic Investigation. 19:73-77.

FEWER PRPC MYELOID-BASED CELLS IN SHEEP WITH THE PRION RESISTANT GENOTYPE - (Peer Reviewed Journal)

Hoesing, L.M., Baszler, T.V., O'Rourke, K.I., Suarez, C.E., Bakko, M., Alverson, J., Knowles, Jr., D.P. 2006. Fewer PrPc myeloid-based cells in sheep with the prion resistant genotype. NeuroReport. 17(2):125-129.

PRPSC ACCUMULATION IN FETAL COTYLEDONS OF SCRAPIE-RESISTANT LAMBS IS INFLUENCED BY FETUS LOCATION IN THE UTERUS - (Peer Reviewed Journal)

Alverson, J., O'Rourke, K.I., Baszler, T.V. 2006. PrPSc accumulation in fetal cotyledons of scrapie-resistant lambs is influenced by fetus location in the uterus. Journal of General Virology. 87:1035-1041.

CHRONIC WASTING DISEASE OF ELK AND DEER AND CREUTZFELDT-JAKOB DISEASE: COMPARATIVE ANALYSIS OF THE SCRAPIE PRION PROTEIN - (Peer Reviewed Journal)

Xie, Z., O'Rourke, K.I., Dong, Z., Allen, J., Langenberg, J., Belay, E.D., Schonberger, L.B., Petersen, R.B., Zou, W., Kong, Q. 2006. Chronic Wasting Disease of Elk and Deer and Creutzfeldt-Jakob Disease: Comparative Analysis of the Scrapie Prion Protein. Journal of Biological Chemistry. 281(7):4199-4206.

COMPARISON OF TWO AUTOMATED IMMUNOHISTOCHEMICAL PROCEDURES FOR THE DIAGNOSIS OF SCRAPIE IN DOMESTIC SHEEP AND CHRONIC WASTING DISEASE IN NORTH AMERICAN WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS) AND MULE DEER (ODOCOILEUS HEMI - (Peer Reviewed Journal)

Baszler, T.V., Kiupel, M., Williams, E.S., Thomsen, B.V., Gidlewski, T., Montgomery, D.L., O'Rourke, K.I., Hall, S.M. 2006. Comparison of two automated immunohistochemical procedures for the diagnosis of scrapie in domestic sheep and chronic wasting disease in north American white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus). Journal of Veterinary Diagnostic Investigation. 18(2):147-155.

PRELIMINARY OBSERVATIONS OF GENETIC SUSCEPTIBILITY OF ELK (CERVUS ELAPHUS NELSONI) TO CHRONIC WASTING DISEASE BY EXPERIMENTAL ORAL INOCULATION - (Peer Reviewed Journal)

Hamir, A.N., Gidlewski, T., Spraker, T.R., Miller, J.M., Creekmore, L., Crocheck, M., Cline, T., Orourke, K.I. 2006. Preliminary observations of genetic susceptibility of elk (Cervus elaphus nelsoni) to chronic wasting disease by experimental oral inoculation. Journal of Veterinary Diagnostic Investigation. 18(1):110-114.

CHRONIC WASTING DISEASE OF ELK: TRANSMISSIBILITY TO HUMANS EXAMINED BY TRANSGENIC MOUSE MODELS - (Peer Reviewed Journal)

Kong, Q., Huang, S., Zou, W., Vanegas, D., Wang, M., Wu, D., Yuan, J., Zheng, M., Bai, H., Deng, H., Chen, K., Allen, J., O'Rourke, K.I., Belay, E.D., Schonberger, L.B., Petersen, R.B., Sy, M., Chen, S.G., Gambetti, P. 2005. Chronic Wasting Disease of Elk: Transmissibility to Humans Examined by Transgenic Mouse Models. The Journal of Neuroscience. 25(35):7944-7949.

ASSESSMENT OF THE GENETIC RISK AND IMPACT OF LATERAL TRANSMISSION IN A VALINE-ASSOCIATED SCRAPIE OUTBREAK IN SHEEP - (Peer Reviewed Journal)

Evoniuk, J.M., Stoltenow, C.L., O'Rourke, K.I., Moore, B.L., Redmer, D.A. 2005. Assessment of the genetic risk and impact of lateral transmission in a valine-associated scrapie outbreak in sheep. American Journal of Veterinary Research. 66(8):1-6.

DEVELOPMENT OF AN ASSAY TO DETERMINE SINGLE NUCLEOTIDE POLYMORPHISMS (SNP) IN THE PRION GENE FOR THE DIAGNOSIS OF GENETIC SUSCEPTIBILITY TO SCRAPIE IN SHEEP - (Proceedings/Symposium)

Johnson, M.L., Evoniuk, J.M., Stoltenow, C.L., Orourke, K.I., Redmer, D.A. 2005. Development of an assay to determine single nucleotide polymorphisms (snp) in the prion gene for the diagnosis of genetic susceptibility to scrapie in sheep. American Society of Animal Science. 56:151.

EVALUATION OF WESTERN BLOTTING METHODS USING SAMPLES WITH OR WITHOUT SODIUM PHOSPHOTUNGSTIC ACID PRECIPITATION FOR DIAGNOSIS OF SCRAPIE AND CHRONIC WASTING DISEASE - (Peer Reviewed Journal)

Huang, H., Rendulich, J., Stevenson, D., O'Rourke, K.I., Balachandran, A. 2005. Evaluation of Western blotting methods using samples with or without sodium phosphotungstic acid precipitation for diagnosis of scrapie and chronic wasting disease. Canadian Journal of Veterinary Research. 69(3):193-199.

Evaluation of Western blotting methods using samples with or without sodium phosphotungstic acid precipitation for diagnosis of scrapie and chronic wasting disease - (Peer Reviewed Journal)

Huang, H., Rendulich, J., Stevenson, D., Orourke, K.I., Balachandran, A. 2005. Evaluation of Western blotting methods using samples with or without sodium phosphotungstic acid precipitation for diagnosis of scrapie and chronic wasting disease. Canadian Journal of Veterinary Research. 69(3):193-199.

EXPERIMENTAL TRANSMISSION OF CHRONIC WASTING DISEASE AGENT TO CATTLE BY INTRACEREBRAL ROUTE - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Cutlip, R.C., Miller, J.M., Orourke, K.I., Williams, E.S., Miller, M.W., Stack, M.J., Chaplin, M.J., Richt, J. 2005. Experimental transmission of chronic wasting disease agent to cattle by intracerebral route. Journal of Veterinary Diagnostic Investigation. 17:276-281.

THE INCIDENCE OF GENOTYPES AT CODON 171 OF THE PRION PROTEIN GENE (PRNP) IN FIVE BREEDS OF SHEEP AND PRODUCTION TRAITS OF EWES ASSOCIATED WITH THOSE GENOTYPES. - (Peer Reviewed Journal)

Alexander, B.M., Stobart, R.H., Russell, W.C., Orourke, K.I., Lewis, G.S., Logan, J.R., Duncan, J.R., Moss, G.E. 2005. The incidence of genotypes at codon 171 of the prion protein gene (prnp) in five breeds of sheep and production traits of ewes associated with those genotypes. Journal of Animal Science. 83(2):455-459.

EXPERIMENTAL TRANSMISSION OF CHRONIC WASTING DISEASE AGENT TO CATTLE BY INTRACEREBRAL ROUTE: FINAL OUTCOME OF THE STUDY - (Abstract)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Cutlip, R.C., Miller, J.M., Orourke, K.I., Williams, E.S., Miller, M.W., Stack, M.J., Chaplin, M.J. 2004. Experimental transmission of chronic wasting disease agent to cattle by intracerebral route: final outcome of the study [abstract]. Animal Prion Diseases and the Americas. p. 78.

EXPERIMENTAL TRANSMISSION OF CHRONIC WASTING DISEASE AGENT TO CATTLE BY INTRACEREBRAL ROUTE: FINAL OUTCOME OF THE STUDY - (Abstract)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Cutlip, R.C., Miller, J.M., O'Rourke, K.I., Williams, E.S., Miller, M.W., Stack, M.J., Chaplin, M.J. 2004. Experimental transmission of chronic wasting disease agent to cattle by intracerebral route: final outcome of the study [abstract]. Annual Conference of the American College of Veterinary Pathologists. 41:588.

BIOLOGY OF PRP-SC ACCUMULATION IN TWO NATURALLY SCRAPIE-INFECTED SHEEP FLOCKS - (Peer Reviewed Journal)

Caplazi, P., O'Rourke, K.I., Wolf, C., Shaw, D., Baszler, T. 2004. Biology of PrP-Sc accumulation in two naturally scrapie-infected sheep flocks. Journal of Veterinary Diagnostic Investigation. 16(6):489-496.

POLYMORPHISMS IN THE PRION PRECURSOR FUNCTIONAL GENE BUT NOT THE PSEUDOGENE ARE ASSOCIATED WITH SUSCEPTIBILITY TO CHRONIC WASTING DISEASE IN WHITE-TAILED DEER - (Peer Reviewed Journal)

O'Rourke, K.I., Spraker, T.R., Hamburg, L.K., Besser, T.E., Brayton, K.A., Knowles, D.P. 2004. Polymorphisms in the prion precursor functional gene but not the pseudogene are associated with susceptibilty to chronic wasting disease in white-tailed deer. Journal of General Virology. 85:1339-1346.

RESISTANCE TO SCRAPIE IN PRP ARR/ARQ HETEROZYGOUS SHEEP IS NOT CAUSED BY PREFERENTIAL ALLELIC USAGE - (Peer Reviewed Journal)

Caplazi, P., Orourke, K.I., Baszler, T.V. 2004. Resistance to scrapie in prp arr/arq heterozygous sheep is not caused by preferential allelic usage. Journal of Clinical Pathology. 57:647-650.

CHRONIC WASTING DISEASE GENETICS AND MOLECULAR BIOLOGY IN THREE CERVID SPECIES - (Proceedings/Symposium) - (15-Nov-03)

A PROCESSED PSEUDOGENE CONTRIBUTES TO APPARENT MULE DEER PRION GENE HETEROGENEITY - (Peer Reviewed Journal)

Brayton, K.A., O'Rourke, K.I., Lyda, A.K., Miller, M.W., Knowles, D.P. A processed pseudogene contributes to apparent mule deer prion gene heterogeneity. Gene. 2003. v. 326. p. 167-173.

VARIABLE PATTERNS OF DISTRIBUTION OF PRP(CWD) IN THE OBEX AND CRANIAL LYMPHOID TISSUES OF ROCKY MOUNTAIN ELK (CERVUS ELAPHUS NELSONI) WITH SUBCLINICAL CHRONIC WASTING DISEASE - (Peer Reviewed Journal)

Spraker, T.R., Balachandran, A., Zhuang, D., O'Rourke, K.I. 2004. Variable patterns of distribution of PrP(CWD) in the obex and cranial lymphoid tissues of Rocky Mountain elk (Cervus elaphus nelsoni) with subclinical chronic wasting disease. Veterinary Record. 155(10):295-302.

TRANSMISSION OF SHEEP SCRAPIE TO ELK (CERVUS ELAPHUS NELSONI) BY INTRACEREBRAL INOCULATION - (Abstract)

Hamir, A.N., Miller, J.M., Cutlip, R.C., Richt, J.A., Kunkle, R.A., O'Rourke, K.I., Jenny, A.L., Stack, M.J., Chaplin, M.J. 2003. Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation. 46th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. p. 210.

EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE (CWD-MULE DEER) TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M.J., Chaplin, M.J., Miller, M., O'Rourke, K.I. 2003. Experimental cross-species transmission of chronic wasting disease (CWD-mule deer) to domestic livestock at the National Animal Disease Center: An update [Abstract]. 46th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. p. 209.

HISTAMINE AS AN AID TO BIOPSY OF THIRD EYELID LYMPHOID TISSUE IN SHEEP - (Peer Reviewed Journal)

Bender, S., Alverson, J., Herrmann, L.M., O'Rourke, K.I. 2004. Histamine as an aid to biopsy of third eyelid lymphoid tissue in sheep. Veterinary Record. 154: 662-663.

TRANSMISSION OF SHEEP SCRAPIE TO ELK (CERVUS ELAPHUS NELSONI) BY INTRACEREBRAL INOCULATION - (Abstract)

Hamir, A.N., Miller, J.M., Cutlip, R.C., Richt, J.A., Kunkle, R.A., O'Rourke, K.I., Jenny, A.L., Stack, M.J., Chaplin, M.J. 2003. Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation [Abstract]. International Conference on Emerging Zoonoses. p. 92.

EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M., Miller, M.W., O'Rourke, K.I., Chaplin, M.J. 2003. Experimental cross-species transmission of chronic wasting disease to domestic livestock at the National Animal Disease Center: An update [Abstract]. International Conference on Emerging Zoonoses. p. 91.

THIRD EYELID LYMPHOID TISSUE BIOPSY IN SHEEP IS AIDED BY HISTAMINE - (Abstract)

Alverson, J., Bender, S., Herrmann, L.M., Orourke, K.I. 2003. Third eyelid lymphoid tissue biopsy in sheep is aided by histamine [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians 46th Annual Conference. 41: 212.

TRANSMISSION OF SHEEP SCRAPIE TO ELK (CERVUS ELAPHUS NELSONI) BY INTRACEREBRAL INOCULATION - (Abstract)

Hamir, A.N., Miller, J.M., Cutlip, R.C., Richt, J.A., Kunkle, R.A., O'Rourke, K.I., Jenny, A.L., Stack, M.J., Chaplin, M.J. 2003. Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation [Abstract]. 52nd Annual Wildlife Disease Association Annual Meeting. p. 135.

EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE (CWD-MULE DEER) TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE - (Abstract)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M., Chaplin, M., Miller, M., O'Rourke, K.I. 2003. Experimental cross-species transmission of chronic wasting disease (CWD-mule deer) to domestic livestock at the National Animal Disease Center: An update [Abstract]. 52nd Annual Wildlife Disease Association Conference. p. 134.

TRANSMISSION OF SHEEP SCRAPIE TO ELK (CERVUS ELAPHUS NELSONI) BY INTRACEREBRAL INOCULATION - (Abstract)

HAMIR, A.N., MILLER, J.M., CUTLIP, R.C., RICHT, J., KUNKLE, R.A., OROURKE, K.I., JENNY, A.L., STACK, M.J., CHAPLIN, M.J. TRANSMISSION OF SHEEP SCRAPIE TO ELK (CERVUS ELAPHUS NELSONI) BY INTRACEREBRAL INOCULATION. DISEASES AT THE INTERFACE BETWEEN DOMESTIC LIVESTOCK AND WILDLIFE SPECIES. 2003. ABSTRACT NO. 6.

EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE (CWD-MULE DEER) TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE - (Abstract)

HAMIR, A.N., CUTLIP, R.C., MILLER, J.M., RICHT, J., KUNKLE, R.A., WILLIAMS, E., STACK, M., CHAPLIN, M., MILLER, M., OROURKE, K.I. EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE (CWD-MULE DEER) TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE. DISEASES AT THE INTERFACE BETWEEN DOMESTIC LIVESTOCK AND WILDLIFE SPECIES. 2003. ABSTRACT NO. 8.

A PRION PROTEIN EPITOPE SELECTIVE FOR THE PATHOLOGICALLY MISFOLDED CONFORMATION - (Peer Reviewed Journal)

Paramithiotis, E., Pinard, M., Lawton, T., Laboissiere, S., Leathers, S., Zou, W., Estey, L., Lamontagne, J., Lehto, M.T., Kondejewski, L.H., Francoeur, G.P., Papadopoulos, M., Haghighat, A., Spatz, S.J., Head, M., Will, R., Ironside, J., Orourke, K.I., Tonelli, Q., Ledebur, H.C., Chakrabartty, A., Cashman, N.R. 2003. A prion protein epitope selective for the pathologically misfolded conformation. Nature Medicine. 9(7):893-899.

DIFFERENCES IN PRODUCTION TRAITS BETWEEN SCRAPIE RESISTANT AND SCRAPIE SUSCEPTIBLE EWES - (Proceedings/Symposium)

Proc. Western Sec. Amer Soc Anim Sci 2003, 54:101

ABUNDANT PRP-CWD IN TONSIL FROM MULE DEER WITH PRECLINICAL CHRONIC WASTING DISEASE - (Peer Reviewed Journal)

O'Rourke, K.I., Zhuang, D., Lyda, A.K., Gomez, G., Williams, E.S., Tuo, W., Miller, M.W. Abundant PrP-CWD in tonsil from mule deer with preclinical chronic wasting disease. Journal of Diagnostic Investigation. 2003. v. 15. 320-323.

TRANSMISSION OF TRANSMISSIBLE MINK ENCEPHALOPATHY (TME) TO RACCOONS (PROCYON LOTOR) BY INTRACEREBRAL INOCULATION - (Peer Reviewed Journal)

Hamir, A.N., Miller, J.M., O'Rourke, K.I., Bartz, J.C., Stack, M.J., Chaplin, M.J. 2004. Transmission of transmissible mink encephalopathy (TME) to raccoons (Procyon lotor) by intracerebral inoculation. Journal of Veterinary Diagnostic Investigation. 16(1):57-63.

PRECLINICAL DIAGNOSIS OF CHRONIC WASTING DISEASE IN CAPTIVE MULE DEER (ODOCOILEUS HEMINONUS) AND WHITE TAILED DEER (ODOCOILEUS VIRGINIANUS) USING TONSILLAR BIOPSY - (Peer Reviewed Journal)

Wild, M.A., Spraker, T.R., Sigurdson, C.J., Orourke, K.I., Miller, M.A. 2002. Preclinical diagnosis of chronic wasting disease in captive mule deer (odocoileus heminonus) and white tailed deer (odocoileus virginianus) using tonsillar biopsy. Journal of General Virology. 83: 2629-2634.

ACTIVE SURVEILLANCE FOR SCRAPIE UTILIZING THIRD EYELID BIOPSY AND GENETIC SUSCEPTIBILITY TESTING IN FLOCKS OF SHEEP IN WYOMING - (Peer Reviewed Journal)

O'Rourke, K.I., Duncan, J.V., Logan, J.R., Anderson, A.K., Norden, D.K., Williams, E.S., Combs, B.A., Stobart, R.H., Moss, G.E., Sutton, D.L. 2002. Active surveillance for scrapie utilizing third eyelid biopsy and genetic susceptibility testing in flocks of sheep in Wyoming. Clinical and Diagnostic Laboratory Immunology. 9(5):966-971.

DISTRIBUTION OF PROTEASE-RESISTANT PRION PROTEIN AND SPONGIFORM ENCEPHALOPATHY IN FREE-RANGING MULE DEER (ODOCOILEUS HEMIONUS) WITH CHRONIC WASTING DISEASE - (Peer Reviewed Journal)

Spraker, T.R., Zink, R.R., Cummings, B.A., Sigurdson, C.J., Miller, M.W., O'Rourke, K.I. Distribution of protease-resistant prion protein and spongiform encephalopathy in free-ranging mule deer (Odocoileus hemionus) with chronic wasting disease. Veterinary Pathology. 2002. v. 39. p. 546-556.

IMMUNOHISTOCHEMICAL DETECTION AND DISTRIBUTION OF PRION PROTEIN IN A GOAT WITH NATURAL SCRAPIE - (Peer Reviewed Journal)

Valdez, R.A., Rock, M.J., Anderson, A.K., O'Rourke, K.I. 2003. Immunohistochemical detection and distribution of prion protein in a goat with natural scrapie. Journal of Veterinary Diagnostic Investigation. 15(2):157-162.

VALIDATION OF MONOCLONAL ANTIBODY F99/97.6.1 FOR IMMUNOHISTOCHEMICAL STAINING OF BRAIN AND TONSIL IN MULE DEER (ODOCOILEUS HEMIONUS) WITH CHRONIC WASTING DISEASE - (Peer Reviewed Journal) - (10-Sep-01)

IMMUNOREACTIVITY OF THE MONOCLONAL ANTIBODY F89/160.1.5 FOR THE HUMAN PRION PROTEIN - (Peer Reviewed Journal)

Van Everbroeck, B., O'Rourke, K.I., Cras, P. 1999. Immunoreactivity of the monoclonal antibody F89/160.1.5 for the human prion protein. Eur. J. Histochem. 43(4):335-338.