Projects

IMMUNOLOGIC AND PHARMACOLOGICAL INTERVENTIONS OF VECTOR-BORNE BABESIOSIS Appropriated (D)   Accession Number: 411502

VACCINE DEVELOPMENT FOR BABESIA BOVIS THROUGH FUNCTIONAL GENOMICS Specific Cooperative Agreement (S)   Accession Number: 412177

Publications

Transfection systems for Babesia bovis: a review of methods for the transient and stable expression of exogenous genes - (Peer Reviewed Journal) - (03-Mar-09)

Stable expression of a gfp-bsd fusion protein in B. bovis merozoites - (Peer Reviewed Journal) - (07-Aug-08)

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle - (Peer Reviewed Journal)

Silva, M., Helali, S., Esseghaierb, C., Suarez, C.E., Oliva, A., Abdelghani, A. 2008. An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle. Sensors and Actuators B: Chemical. 135:206-213.

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle - (Peer Reviewed Journal)

Goff, W.L., Johnson, W.C., Molloy, J.B., Jorgensen, W.K., Waldron, S.J., Figueroa, J.V., Matthee, O., Adams, D.S., Mcguire, T.C., Pino, I., Mosqueda, J., Palmer, G.H., Suarez, C.E., Knowles Jr, D.P., Mcelwain, T.F. 2008. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Babesia bigemina Antibodies in Cattle. Clinical and Vaccine Immunology. 15(9):1316-1321.

Transient transfection of purified Babesia bovis merozoites - (Peer Reviewed Journal)

Suarez, C.E., Mcelwain, T.F. 2008. Transient transfection of purified Babesia bovis merozoites. Experimental Parasitology. 118(4):498-504.

Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa - (Peer Reviewed Journal)

Brayton, K., Lau, A.O., Herndon, D.R., Hannick, L., Kappmeyer, L.S., Berens, S.J., Bidwell, S.L., Brown, W.C., Crabtree, J., Fadrosh, D., Feldblum, T., Forberger, H.A., Haas, B.J., Howell, J.M., Khouri, H., Koo, H., Mann, D.J., Norimine, J., Paulsen, I.T., Radune, D., Ren, Q., Smith, R.K., Suarez, C.E., White, O., Wortman, J.R., Knowles Jr, D.P., Mcelwain, T.F., Nene, V.M. 2007. Genome Sequence of Babesia bovis and Camparative Analysis of Apicomplexan Hemoprotozoa. PLoS Pathogens. 3(10):1401-1413.

Evidence for a relationship between bovine erythrocyte lipid membrane peculiarities and immune pressure from ruminal ciliates - (Peer Reviewed Journal) - (03-May-07)

Optimizacion of Babesia bovis transfection methods - (Monograph)

Suarez, C.E., Laughery, J., Gonzalez, M.G., Mcelwain, T. 2007. Optimizacion of Babesia bovis transfection methods. Parassitologia. 49(Suppl. 1):67-70.

Stable expression of a GFP-BSD fusion protein in transfected and blasticidin-selected B. bovis merozoites - (Abstract)

Suarez, C.E., Mcelwain, T.F. 2007. Stable expression of a GFP-BSD fusion protein in transfected and blasticidin-selected B. bovis merozoites. Molecular Parasitology Meeting XVIII, September 16-20, Woods Hole, MA.

Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization - (Monograph)

Wilkowsky, S., Farber, M., Echaide, I., Mosqueda, J., Alcaraz, E., Suarez, C.E., Florin-Christensen, M. 2007. Taking advantage of the polymorphism of the MSA-2 family for Babesia bovis strain characterization. Parassitologia. 49(Suppl. 1):63-66.

Search for Babesia bovis vaccine candidates - (Monograph)

Florin-Christensen, M., Schnittger, L., Dominguez, M., Mesplet, M., Rodriguez, A., Ferreri, L., Asenzo, G., Wilkowsky, S., Farber, M., Echaide, I., Suarez, C.E. 2007. Search for Babesia bovis vaccine candidates. Parassitologia. 49(Suppl. 1):9-12.

Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis - (Peer Reviewed Journal)

Goff, W.L., Molloy, J.B., Johnson, W.C., Suarez, C.E., Pino, I., Rhalem, A., Sahibi, H., Ceci, L., Carelli, G., Adams, D.S., Mcguire, T.C., Knowles Jr, D.P., Mcelwain, T.F. 2006. Validation of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Babesia bovis. Clinical and Vaccine Immunology. 13(11):1212-1216.

CHARACTERIZATION AND GENE EXPRESSION OF BABESIA BOVIS ELONGATION FACTOR-1ALPHA - (Peer Reviewed Journal)

Suarez, C.E., Norimine, J., Lacy, P.A., Mcelwain, T.F. 2006. Characterization and gene expression of Babesia bovis elongation factor-1alpha. International Journal for Parasitology. 36(8):965-973.

PROSPECTS FOR RECOMBINANT VACCINES AGAINST BOVINE BABESIOSIS. - (Peer Reviewed Journal)

Brown, W.C., Norimine, J., Goff, W.L., Suarez, C.E., Mcelwain, T.F. 2006. Prospects for recombinant vaccines against bovine babesiosis. 2006. Parasite Immunology. 28:315-327.

FEWER PRPC MYELOID-BASED CELLS IN SHEEP WITH THE PRION RESISTANT GENOTYPE - (Peer Reviewed Journal)

Hoesing, L.M., Baszler, T.V., O'Rourke, K.I., Suarez, C.E., Bakko, M., Alverson, J., Knowles, Jr., D.P. 2006. Fewer PrPc myeloid-based cells in sheep with the prion resistant genotype. NeuroReport. 17(2):125-129.

NEOSPORA CANINUM: ANTIBODIES DIRECTED AGAINST TACHYZOITE SURFACE PROTEIN NCSRS2 INHIBIT PARASITE ATTACHMENT AND INVASION OF PLACENTAL TROPHOBLASTS IN VITRO - (Peer Reviewed Journal)

Haldorson, G., Stanton, J., Mathison, B.A., Suarez, C.E., Baszler, T. 2006. Neospora caninum: antibodies directed against tachyzoite surface protein ncsrs2 inhibit parasite attachment and invasion of placental trophoblasts in vitro. Experimental Parasitology. 112(3):172-178.

IDENTIFICATION OF VACCINE CANDIDATE PEPTIDES IN THE NCSRS2 SURFACE PROTEIN OF NEOSPORA CANINUM BY USING CD4+ CYTOTOXIC T LYMPHOCYTES AND GAMMA INTERFERON-SECRETING T LYMPHOCYTES OF INFECTED HOLSTEIN CATTLE - (Peer Reviewed Journal)

Staska, L.M., Davies, C.J., Brown, W.C., Mcguire, T.C., Suarez, C.E., Park, J.Y., Mathison, B.A., Abbott, J.R., Baszler, T.V. 2005. Identification of vaccine candidate peptides in the ncsrs2 surface protein of neospora caninum by using cd4+ cytotoxic t lymphocytes and gamma interferon-secreting t lymphocytes of infected holstein cattle. Infection and Immunity. 73(3):1321-1329.

USE OF A MONOCLONAL ANTIBODY AGAINST BABESIA BOVIS MEROZOITE SURFACE ANTIGEN-2C FOR THE DEVELOPMENT OF A COMPETITIVE ELISA TEST. - (Proceedings/Symposium)

Dominguez, M., Zabal, O., Wilkowsky, S., Echaide, I., Torioni, D.E., Asenzo, G., Rodriguez, A., Zamorano, P., Farber, M., Suarez, C.E., Florin-Christensen, M. 2004. Use of a monoclonal antibody against babesia bovis merozoite surface antigen-2c for the development of a competitive elisa test. Annals Of The New York Academy Of Sciences. 1026:165-170.

INTERGENIC REGIONS IN THE RHOPTRY ASSOCIATED PROTEIN-1 (RAP-1) LOCUS PROMOTE EXOGENOUS GENE EXPRESSION IN BABESIA BOVIS. - (Peer Reviewed Journal)

Suarez, C.E., Palmer, G.H., LeRoith, T., Florin-Christensen, M., Crabb, B., McElwain, T.F. 2004. Intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in Babesia bovis. International Journal for Parasitology. 34:1177-1184.

EARLY IMMUNE RESPONSES IN BOVINES EXPERIMENTALLY INFECTED WITH THE HEMOPARASITE BABESIA BOVIS - (Abstract)

Asenzo, G., Dominguez, M., Rodriguez, A., Echaide, I., Wilkowsky, S., Suarez, C.E., Zamorano, P., Florin-Christensen, M. 2003. Early immune responses in bovines experimentally infected with the hemoparasite babesia bovis [abstract]. Biocell. 27:1-172.

DEVELOPMENT OF A NEW SYSTEM FOR BABESIA BOVIS STRAIN IDENTIFICATION USING MOLECULAR MARKERS - (Abstract)

Wilkowsky, S., Dominguez, M., Farber, M., Echaide, I., Valentini, I., Alcaraz, E., Cetra, B., Suarez, C.E., Florin-Christensen, M. 2003. Development of a new system for babesia bovis strain identification using molecular markers [abstract]. Biocell. 27:1-172.

DEVELOPMENT OF A COMPETITIVE ELISA TEST FOR BABESIA BOVIS INFECTIONS BASED ON MEROZOITE SURFACE ANTIGEN-2C - (Abstract)

Dominguez, M., Zabal, O., Wilkowsky, S., Rodriguez, A., Asenzo, G., Farber, M., Echaide, I., Echaide, S.T., Suarez, C.E., Florin-Christensen, M. 2003. Development of a competitive elisa test for babesia bovis infections based on merozoite surface antigen-2c [abstract]. Biocell. 27:1-172.

STIMULATION OF T-HELPER CELL GAMMA INTERFERON AND IMMUNOGLOBULIN G RESPONSES SPECIFIC FOR BABESIA BOVIS RHOPTRY-ASSOCIATED PROTEIN 1 (RAP-1) OR A RAP-1 PROTEIN LACKING IN THE CARBOXY-TERMINAL REPEAT REGION IS INSUFFICIENT... - (Peer Reviewed Journal)

Norimine, J., Mosqueda, J., Suarez, C.E., Palmer, G.H., Mcelwain, T.F., Mbassa, G., Brown, W.C. 2003. Stimulation of t-helper cell gamma interferon and immunoglobulin g responses specific for babesia bovis rhoptry-associated protein 1 (rap-1) or a rap-1 protein lacking in the carboxy-terminal repeat region is insufficient to provide protective immunity again virulent B. bovis challange. Infection and Immunity. 2003. 71(9):5021-5032.

BABESIA BOVIS MEROZOITE SURFACE PROTEIN-2C (MSA-2C) CONTAINS HIGHLY IMMUNOGENIC, CONSERVED B-CELL EPITOPES THAT ELICIT NEUTRALIZATION-SENSITIVE ANTIBODIES IN CATTLE - (Peer Reviewed Journal) - (26-Dec-02)

ORGANIZATION, TRANSCRIPTION, AND EXPRESSION OF RHOPTRY ASSOCIATED PROTEIN GENES IN THE BABESIA BIGEMINA RAP-1 LOCUS - (Peer Reviewed Journal) - (17-Dec-02)

COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY BASED ON A RHOPTRY-ASSOCIATED PROTEIN 1 EPITOPE SPECIFICALLY IDENTIFIES BABESIA BOVIS-INFECTED CATTLE - (Peer Reviewed Journal)

Goff, W.L., McElwain, T.F., Suarez, C.E., Johnson, W.C., Brown, W.C., Norimine, J., Knowles, D.P. Competitive enzyme-linked immunosorbent assay based on a rhoptry-associated protein-1 epitope specifically identifies Babesia bovis-infected cattle. Clinical and Diagnostic Laboratory Immunology. 2002. v. 10. p. 38-43.

THE BABESIA BOVIS MEROZOITE SURFACE ANTIGEN 2 LOCUS CONTAINS FOUR TANDEMLY ARRANGED AND EXPRESSED GENES ENCODING IMMUNOLOGICALLY DISTINCT PROTEINS - (Peer Reviewed Journal) - (17-Apr-02)

IMMUNODOMINANT EPITOPES IN BABESIA BOVIS RHOPTRY ASSOCIATED PROTEIN-1 THAT ELICIT MEMORY CD4+-T-LYMPHOCYTE RESPONSES IN B. BOVIS IMMUNE INDIVIDUALS ARE LOCATED IN THE AMINO-TERMINAL DOMAIN - (Peer Reviewed Journal) - (02-Jan-02)