Laboratory of Dr. Jack Staub: Cucumber and Melon Genetics

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Table of Contents

• About Dr. Staub
• Research Objectives and Methodology of the Cucumber and Melon Project
• Expected Results from Research in the USDA Laboratory
• What is Known in the Research Area (an overview)
• State of the Art (an overview)
• SSR development
• Marker-Assisted Selection
• Laboratory Accomplishments (an overview)
• Demonstrated Accomplishments of the Laboratory
  1. Germplasm Enhancement
     • Figure 1. Map position of a regulatory enzyme (ACC synthase) closely closely linked (associated) to female gene (F) which controls its expression.
     • Varietial releases
     • Figure 2. Released lines and cultivars have multiple disease resistance and possess unique plant habits introgressed from exotic germplasm
  2. The Development of Genetic Stocks, Genetic Analyses, and Map Construction
     • Cucmber map information
     • Figure 3. Cucumber gene order and map distance from F2 populations
     • Figure 4. Linkage map for 12 isozyme loci based on F2 and BC populations of cucumber
     • Figure 5. Linkage groups detected in the cross Gy-14 x PI 183967
     • Figure 6. Markers, enzymes, linkage groups, and Kosambi distances detected in the cross Gy-14 x PI 432860
     • Figure 7. A linkage map in cucumber constructed from the segregation of isozymes and morphological traits in 28 F2 families
     • Figure 8. A RAPD-based linkage map in cucumber
     • Figure 9. Narrow-based (cultivated Cucumis sativus) consensus map.
     • Figure 10. Wide based (Cucumis sativus x C. s. var. hardwickii) consensus map.
     • Melon Map Information
  3. Cucumis Taxonomy, Evolution, and Interspecific Hybridization
  4. Molecular Marker Utility for PVP and Diversity Analysis\
  5. Trait Evaluation and Collection of Exotic Germplasm
  6. Germplasm Evaluation and Genetic Diversity
     • Figure 11. Screening for resistance to heat stress.
  7. Feasibility Studies for Cucumber Production
  8. The Development of Molecular Markers
  9. Identification and Characterization of a Postharvest Disorder
     • Figure 12. Pillowy fruit disorder in cucumber.
     • Figure 13. Various intensities of pillowy in infected cucumber fruit.
     • Figure __. The photo of cuke slices
  10. Characterization of Responses to Stress Environments
      • Figure 14. Response of cucumber plants to command herbicide injury
      • Figure 15. Genotype differences in response to water stress. Comparison of nearly-isogenic lines.
      • Figure 16. Misshapen fruit as a result of water stress.
  11. Development of Computer Programs for Research and Teaching
      • Figure 17. Table of contents for the laboratory manual, Crossover.
• Publications
• Data from publications
• Files for cooperators

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