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Library Info Page
| Library Name: | Lupski_dorsal_root_ganglion |
| Organism: | Homo sapiens |
| UniGene Library ID: | 5655 |
| Tissue Description: | Dorsal root ganglion |
| Library Keywords: | normal, non-normalized, bulk, adult, dorsal root ganglion, CGAP, EST, MGC, male, size fractionated, plasmid vector, directionally cloned, oligo-dT primed |
| #Clones Generated to Date: | 3154 |
| #Sequences Generated to Date: | 9180 |
| Library Preparation Details |
| Description: | cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.7 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine) and is available through Life Technologies. |
| R. Site1: | NotI |
| R. Site2: | SalI |
| Lab Host: | DH10B |
| Vector: | pCMV-SPORT6 (Life Technologies) |
| Vector Type: | plasmid (ampicillin resistant, 200 ug/ml) |
| Tissue Supplier: | Dr. James Lupski |
| Library Producer: | Lupski Laboratory |
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