Project Number: 3625-32000-087-05
Project Type: Reimbursable

Start Date: Nov 01, 2006
End Date: May 01, 2008

Objective:
The objective of this project is to identify changes that occur in gene expression in porcine alveolar macrophages (PAMs) following PRRSV infection. Infection by PRRSV causes significant changes in the function of PAMs by mechanisms that are not understood. New technologies have been developed that permit examination of the expression of genes in fine detail. We will use one such technology, serial analysis of gene expression (SAGE) to obtain a global overview of gene expression to address how PRRSV infection causes immunosuppression and the establishment of a chronically infected state. This gene expression information can be exploited in a number of ways including development of diagnostic assays, understanding molecular mechanism(s) behind disease states and formulating intervention regimens to inhibit or minimize infections and negative outcomes. This study is an initial look at the effect of PRRSV on PAMs and will be the starting point for a more detailed study of PAMs at various stages of an acute infection as well as during the chronic infection stage of the disease.

Approach:
1. Construct and sequence SAGE libraries made from mRNA derived from noninfected and PRRSV-infected PAMs. 2. Determine normal gene expression levels in noninfected PAMs. 3. Identify genes with altered expression levels in PRRSV-infected PAMs that interfere with the function of these cells and/or the formation of a protective immune response. Identify gene products that may be exploited as early diagnostic markers or vaccination targets.