Project Number: 5430-21000-006-09
Project Type: Specific Cooperative Agreement

Start Date: Jul 01, 2005
End Date: Jun 30, 2010

Objective:
1. Conduct genetic mapping studies on Lr12 and Lr42. 2. Conduct marker-assisted selection for Karnal bunt resistance genes. 3. Develop resources and techniques for identification of targeted wheat mutants by TILLING.

Approach:
A collaborative program will be conducted to tag and identify genes that control avirulence in the wheat leaf rust fungus. Biolistics will be used to tag genes within the leaf rust fungus genome and sequencing will be used to identify candidate avirulence genes. Resistance genes for important wheat diseases will be genetically mapped and markers will be developed for marker-assisted selection. Microsatellites, amplified fragment length polymorphisms, and expressed sequenced tags will be used as sources of molecular markers. Physical maps will be constructed using genomic libraries and electrophoresis.