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Research Project:
MOLECULAR BASIS FOR THE DEVELOPMENT OF SANITIZER TOLERANCE IN LISTERIA MONOCYTOGENES
Location: Bacterial Epidemiology and Antimicrobial Resistance
Project Number: 6612-41420-015-05
Project Type:
Specific Cooperative Agreement
Start Date: Sep 30, 2005
End Date: Sep 30, 2009
Objective:
The goal of this cooperative research project is to determine the molecular basis for the development of sanitizer tolerance in Listeria monocytogenes.
One objective this research is to determine gene expression differences in planktonic and biofilm Listeria monocytogenes subjected to oxidative and quaternary ammonium sanitizer stresses.
A second objective is to assess the contribution of genes up-regulated in response to oxidative and quaternary ammonium stress to biofilm formation and stress tolerance.
Approach:
Gene expression information for two strains of L. monocytogenes will be obtained by microarray hybridization analyses of mRNA from the following cell types:
Untreated planktonic cells
Untreated biofilm cells
Biofilm cells treated with hydrogen peroxide
Biofilm cells treated with QAC
Planktonic cells treated with hydrogen peroxide
Planktonic cells treated with QAC
Gene expression comparisons will include:
Planktonic versus biofilm cells
Planktonic cells before versus after hydrogen peroxide exposure
Planktonic cells before versus after QAC exposure
Biofilm cells before versus after hydrogen peroxide exposure
Biofilm cells before versus after QAC exposure
The role of genes will be confirmed by constructing specific mutations of genes indicated by microarray analysis and determining the phenotype of the mutants.
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Last Modified: 10/21/2008
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