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SAGE Library Info

Library ID

New SAGE Library Name:	SAGE_Retina_Peripheral_normal_B_4Peri
Old SAGE Library Name:
Organism:	Homo sapiens
Library Keywords:	retina, normal peripheral retina, normal, bulk, human, female, adult, short sage

Tag Info

#Total tags:	126,738
#Tags excluding linkers:	126,691
#Unique tags:	47,840

Tissue Info

Tissue:	retina
Cell/Histology type:	normal peripheral retina
Tissue preparation:	bulk
Mutations:
Patient age:	28-66 year old pool of 5 donors
Sex:	female
Other info:	Libraries were constructed using RNA isolated from pooled 4 mm diameter punches of the macula (encompassing the fovea) and matched 4 mm diameter punches of pooled mid-peripheral retina from the same five donors designated, "4Mac" and "4Peri", respectively.

Library Preparation Info

Tagging enzyme:	BsmFI
Anchoring enzyme:	NlaIII
Tissue or cell line supplier:
Library preparer:	Jessica Ebright
Prepared in lab of:	Catherine Bowes Rickman, Duke University Eye Center
References:	Bowes Rickman C and Ebright JN, et al. Defining the Human Macula Transcriptome and Candidate Retinal Disease Genes Using EyeSAGE. Invest Ophthalmol Vis Sci. 2006 Jun;47(6):2305-16.