Project Number: 3607-22000-011-01
Project Type: Specific Cooperative Agreement

Start Date: Jul 28, 2008
End Date: Feb 28, 2009

Objective:
To identify criteria for evaluating soybean germplasm for partial resistance to BPMV.

Approach:
Our objective is to identify criteria for evaluating soybean germplasm for partial resistance to BPMV. For both the Ohio and the South Dakota projects, funds will be used for a third year of field trials. Experimental approaches and measures of partial resistance will be consistent with those used in the first two years of the study. Briefly, we will examine the effect of Bean pod mottle virus (BPMV) inoculation on 15 Ohio, one Wisconsin and two South Dakota soybean lines and cultivars. Eight replicate plots will be planted, four of which will be inoculated with an Ohio isolate of BPMV (subgroup II) at the unifoliate stage. The other plots will remain uninoculated. Insects will be controlled throughout the season. Plots will be evaluated for disease symptoms, pod number and seeds/pod in the center five nodes. Samples of emerging leaves will be collected 3-6 weeks post-inoculation with BPMV, frozen and stored at -80°C for analysis of BPMV titer. Yield, mottling, BPMV titer in seed, and seed oil and protein content will be assessed. BPMV titer will be determined using quantitative ELISA and/or qRT-PCR assays during the fall/winter. In South Dakota, 8 row split plots will be used with one half of the plot being inoculated with BPMV sap extract (1:10 BPMV infected Phaseolus vulgaris cv. Provider: 0.02 M Na and K phosphate buffer, pH 7.2) by high pressure inoculation. Five South Dakota cultivars and lines will be evaluated 1161RR/SCN, 3219R, LD05-16137, SDXR-017-52, and SDXR-020-18. Data collected will include disease severity ratings, height measurements, node counts, seed and pod counts, agronomic traits¿yield, test weight, etc., and maturity dates and lodging.