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Citation Volume 8, Number 4, April 2002 Research Biofilm on Ventriculo-Peritoneal Shunt Tubing as a Cause of Treatment Failure in Coccidioidal MeningitisLarry E. Davis,*† Guy Cook,‡ and J. William Costerton§ |
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Figure 2. The upper left image shows a section from the tip of the shunt tubing stained with SYTOX Green nucleic acid stain and examined by scanning confocal microscopy with argon-ion laser light source. This specific staining for nucleic acids clearly shows the presence of a biofilm and some 4- to 6-µm cells. The upper right image shows an unstained, transmitted light microscopic image of the same area of the edge of the tubing. The bottom right image shows a recombined image with the nucleic acid stain colocalized with the transmitted light image. The recombined image shows that a substantial (~30 µm) biofilm composed of 4- to 6-µm cells has colonized the “scalloped” surface of this tubing. (x630 total magnification mosaic) |
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This page last reviewed June 21, 2002 |
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