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Research Project:
IRON DEFICIENCY CHLOROSIS: GETTING TO THE ROOT OF THE PROBLEM
Location: Corn Insects and Crop Genetics Research
Project Number: 3625-21220-004-02
Project Type:
Reimbursable
Start Date: Jun 25, 2007
End Date: Oct 24, 2008
Objective:
1. Molecular Marker Discovery: Develop molecular markers that have diagnostic potential in identifying IDC efficient genotypes, extend the usefulness of markers to a broader range of soybean germplasm, and make the markers available to soybean breeders.
2. Candidate Gene Discovery: Use state of the art genomic technologies and the forthcoming whole genome sequence of soybean to identify genes potentially involved in IDC efficiency or inefficiency. Convert these candidate genes into markers that can be used by soybean breeders.
Approach:
Near-isogenic lines representing iron efficient and inefficient genotypes will be grown under iron stress conditions in a hydroponic solution. Affymetrix GeneChips will be used by the Ames and the St. Paul groups to identify genes differentially expressed between the lines and thus, candidates for involvement in the iron stress response. SNPs will be identified in candidate genes and developed as molecular markers. These markers will be tested in a broad range of germplasm to look for association with iron efficiency. Viable markers will be provided to the public and relevant information will be placed into SoyBase, The Soybean Breeder's Toolbox.
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Last Modified: 10/20/2008
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