Project Number: 3625-21000-052-04
Project Type: Specific Cooperative Agreement

Start Date: Sep 06, 2007
End Date: Jun 30, 2010

Objective:
Additional research objectives include 1) develop repeat junction markers for mapping and identification of homoeologous centromeres in soybean, 2) anchor repetitive DNA to the soybean genetic map and whole-genome draft sequence, and 3) identify genomic rearrangements and gene or gene fragments caused by transposable elements.

Approach:
Putative repeat markers will be recognized by analysis of the distribution and organization of transposable elements in the draft sequence of the soybean genome; Polymorphisms will be tested by PCR amplification and transposon-display of parental lines of a segregation population, and the marker exhibiting polymorphisms between parental lines will be mapped using the segregation population and integrated to the draft sequence of the soybean genome; Genomic rearrangements and gene or gene fragments caused by transposable elements will be identified by a combined approach to transposon and gene annotation.