Project Number: 3625-21000-052-01
Project Type: Reimbursable

Start Date: Jul 01, 2007
End Date: Jun 30, 2009

Objective:
1) Assemble the DNA sequence and connect it to the soybean genetic 'road map'. 2) Additional SNP genetic markers will be developed and mapped.

Approach:
Whole genome sequence (WGS) will be overlaid onto the physical and genetic map. Paired ends will be identified that indicate DNA clones span gaps in the genome sequence. BAC end sequences will be aligned to the WGS thus overlaying the sequence onto the physical map. Genome sequence from PI 468916 will be aligned with the WGS of Williams 82 and SNPS will be identified. Information will be provided to collaborators at USDA-ARS, Beltsville and SNP markers will be developed and mapped. A 200 SSR map infrastructure will be created in a cross population between Williams 82 and PI 468916. Approximately 4,000 SNPs will be mapped. The DNA sequence from which the SNPs were detected will be correlated with the WGS, thus anchoring the sequence map with the genetic map. Data will be entered into public soybean genome databases.