Volume 9, Number 2, February 2003

Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates

Wieland Meyer,* Alexandra Castañeda,† Stuart Jackson,*‡ Matthew Huynh,* Elizabeth Castañeda,† and the IberoAmerican Cryptococcal Study Group
*University of Sydney, Sydney, Australia; †Instituto Nacional de Salud, Bogotá, Colombia; and ‡University of Western Sydney, Campbelltown, Australia

Figure 3. Polymerase chain reaction (PCR) fingerprints generated with the primer M13 (A), and URA5 gene restriction fragment length polymorphism (RFLP) profiles identified by double digest of the gene with Sau96I and HhaI (B) obtained from a selection of Mexican Cryptococcus neoformans isolates, given as a representative example for the patterns obtained from clinical and environmental isolates from Latin America. Standard patterns obtained from the reference strains of the major molecular types by PCR-fingerprinting patterns with the microsatellite specific primer M13 as a single primer in the PCR (right-hand side of A) and URA5 gene RFLP profiles generated after double digest with Sau96I and HhaI (right-hand side of B) (VNIII correspond to the seven-band URA5 RFLP pattern and VNIII* correspond to the six-band URA5 RFLP pattern).

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This page last reviewed March 20, 2003

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention