Projects

MANAGEMENT OF CITRUS AND DATE GENETIC RESOURCES AND INFORMATION Appropriated (D)   Accession Number: 413154

ACQUISITION AND EVALUATION OF CITRUS AND CITRUS RELATIVES IN THE CITRUS VARIETY COLLECTION Specific Cooperative Agreement (S)   Accession Number: 412213

USE OF MOLECULAR MARKERS TO DETERMINE GENETIC RELATIONSHIPS OF CITRUS AND CITRUS RELATIVES Specific Cooperative Agreement (S)   Accession Number: 413133

HUANGLONGBING: DEVELOPMENT OF INFORMATION NEEDED FOR AVOIDANCE/MANAGEMENT Trust (T)   Accession Number: 413003

RECOVERY OF CITRUS GERMPLASM IN FLORIDA Trust (T)   Accession Number: 416630

Publications

Phylogenetic analysis of Rutaceous plants based on single nucleotide polymorphism in chloroplast and nuclear gene sequences - (Abstract) - (01-Dec-08)

Gene expression analysis to understand cold tolerance in citrus - (Abstract) - (01-Dec-08)

Biological Indexing of Graft Transmissible Diseases of Citrus - (Book/Chapter) - (10-Jul-08)

Certification Programs for Citrus - (Book/Chapter) - (10-Jul-08)

Production of Polyclonal Antibodies to the Recombinant Coat Protein of Citrus tristeza virus and Their Effectiveness for Virus Detection - (Peer Reviewed Journal)

Iracheta-Cardenas, M.M., Sandoval-Alejo, B.D., Roman-Calderon, M.E., Keremane, M.L., Lee, R.F., Rocha-Pena, M.A. 2008. Production of Polyclonal Antibodies to the Recombinant Coat Protein of Citrus tristeza virus and Their Effectiveness for Virus Detection. Journal of Phytopathology, 156: 243-250

Detection of Candidatus Liberibacter asiaticus in Diaphorina citri and its importance in the management of Citrus Huanglongbing in Florida - (Peer Reviewed Journal)

Keremane, M.L., Halbert, S.E., Ramadugu, C., Webb, S., Lee, R.F. 2008. Detection of Candidatus Liberibacter asiaticus in Diaphorina citri and its importance in the management of Citrus Huanglongbing in Florida. Phytopathology, 98: 387-396

Beginning with Healthy Plants: The Cornerstone of Integrated Pest Management for Perennial Crops - (Proceedings/Symposium)

Lee, R.F. 2007. Beginning with Healthy Plants: The Cornerstone of Integrated Pest Management for Perennial Crops. Proceedings of the International Conference on the Future of Agriculture: Science, Stewardship and Sustainability, Aug. 7-9, 2006, Sacramento, CA. 14 page - CD Rom Publication.

Phylogenetic analysis of Citrus based on sequences of six nuclear genes - (Abstract) - (16-Oct-07)

Transgenic Resistance to Citrus tristeza virus in Grapefruit - (Peer Reviewed Journal)

Lee, R.F., Febres, V.J., Moore, G.A. 2008. Transgenic Resistance to Citrus tristeza virus in Grapefruit. Plant Cell Reports, 27: 93-104

Use of Psyllids in Early Identification of Huanglongbing Disease of Citrus - (Abstract)

Keremane, M.L., Halbert, S., Ramadugu, C., Webb, S., Lee, R.F. 2007. Use of Psyllids in Early Identification of Huanglongbing Disease of Citrus. Phytopathology, 97, No7: S70.

Incidence of Huanglongbing in Psyllids Under Natural Conditions in Florida - (Abstract)

Ramadugu, C., Keremane, M.L., Ramos, C., Halbert, S., Lee, R.F. 2007. Incidence of Huanglongbing in Psyllids Under Natural Conditions in Florida. Phytopathology, 97, No7: S96.

Psyllids as A Tool in Evaluating the Efficiency of Different Management Practices for Control of Citrus Huanglongbing Disease - (Abstract)

Keremane, M.L., Harakava, R., Ramadugu, C., Halbert, S., Yamamoto, P., Lee, R.F. 2007. Psyllids as A Tool in Evaluating the Efficiency of Different Management Practices for Control of Citrus Huanglongbing Disease. Phytopathology, 97, No7: S70.

The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus (CCLV) and its Genome Organization - (Abstract)

Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. The Complete Sequence of the Cytoplasmic Citrus Leprosis Virus, (CCLV) and its Genome Organization. Phytopathology 97: S 174

Evaluación de Anticuerpos Desarrollados Contra la Proteína Recombinante de la Cápside del Virus Tristeza de los Cítricos - (Peer Reviewed Journal)

Sandoval-Alejo, B.D., Iracheta-Cardenas, M.M., Keremane, M.L., Lee, R.F., Rocha-Pena, M.A. 2006. Evaluación de Anticuerpos Desarrollados Contra la Proteína Recombinante de la Cápside del Virus Tristeza de los Cítricos. Revista Mexicana de Fitopatologia, Vol. 24: 137-144.

Ecoport Slide Shows on the Internet Related to Citrus and Citrus Diseases - (Proceedings/Symposium)

Roistacher, C.N., Lee, R.F., Putter, C.A., Putter, R.C. 2007. Ecoport Slide Shows on the Internet Related to Citrus and Citrus Diseases. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 473-476.

Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection - (Abstract)

Keremane, M.L., Ramadugu, C., Warren, C., Niblett, C.L., Lee, R.F. 2007. Complete Nucleotide Sequence of a New Genotype of Citrus Tristeza Virus from an Isolate Having a Mixed Infection. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 487.

Molecular Characterization of Citrus Tristeza Virus Isolates from Mild Strain Cross Protection Experiments in Peru - (Abstract)

Ramos, C., Roistacher, C.N., Muller, G.W., Bederski, K., Rangel, B., Lee, R.F. 2007. Molecular Characterization of Citrus Tristeza Virus Isolates from Mild Strain Cross Protection Experiments in Peru. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 492.

Leprosis in Guatemala - (Abstract)

Palmieri, M., Donis, I., Salazar, A., Blanco, S., Porres, M., Brlansky, R.H., Guerra-Moreno, A.S., Keremane, M.L., Lee, R.F. 2007. Leprosis in Guatemala. International Organization of Citrus Virologists Proceedings, 16th Conference, Pg. 510.

GUS Gene Expression Driven by A Citrus Promoter in Transgenic Tobacco and 'Valencia' Sweet Orange - (Peer Reviewed Journal)

De Azevedo, F.A., Mourao-Filho, De A., Schinor, E.H., De Paoli, L.G., Januzzi-Mendes, B.M., Harakava, R., Gabriel, D.W., Lee, R.F. 2006. GUS Gene Expression Driven by A Citrus Promoter in Transgenic Tobacco and 'Valencia' Sweet Orange. Pesquisa Agropecuaria Brasileira, 41:1623-1628.

HUANGLONGBING, CITRUS GREENING: PREPARING FOR THE FUTURE - (Trade Journal)

Lee, R.F., Keremane, M.L., Williams, M., Vidalakis, G. 2006. Huanglongbing, citrus greening: preparing for the future. Subtropics newsletter.

Citrus Leprosis Symptoms Can be Associated with the Presence of Two Different Viruses: Cytoplasmic and Nuclear, the Former Having a Multipartite RNA Genome - (Proceedings/Symposium)

Guerra-Moreno, A.S., Keremane, M.L., Brlansky, R.H., Lee, R.F. 2007. Citrus Leprosis Symptoms Can be Associated with the Presence of Two Different Viruses: Cytoplasmic and Nuclear, the Former Having a Multipartite RNA Genome. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 230-239.

CITRUS TRISTEZA VIRUS TRANSMISSION BY THE TOXOPTERA CITRICIDA VECTOR: IN VITRO ACQUISITION AND TRANSMISSION AND INFECTIVITY IMMUNONEUTRALIZATION EXPERIMENTS - (Peer Reviewed Journal)

Herron, C.M., Mirkov, T.E., Da Graca, J.V., Lee, R.F. 2006. Citrus tristeza virus transmission by the toxoptera citricida vector: in vitro acquisition and transmission and infectivity immunoneutralization experiments. Journal of Virological Methods.

DEVELOPEMENT OF STABLE, UNIFORM CONTROLS FOR USE IN ELISA ASSAYS FOR CITRUS TRISTEZA VIRUS - (Proceedings/Symposium) - (22-Nov-05)

Molecular Characterization of Citrus tristeza virus Isolates from Panama - (Proceedings/Symposium)

Ramos, C., Castillo, J.C., Fernandez, O., Rangel, B., Keremane, M.L., Lee, R.F. 2007. Molecular Characterization of Citrus tristeza virus Isolates from Panama. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 159-164.

Citrus Viruses in Guatemala: Application of Laboratory-Based Assays - (Proceedings/Symposium)

Palmieri, M., Donis, I., Salazar, A.L., Cruz, N., Paniagua, A., Brlansky, R.H., Guerra-Moreno, A., Keremane, M.L., Ballance, P.M., Lee, R.F. 2007. Citrus Viruses in Guatemala: Application of Laboratory-Based Assays. International Organization of Citrus Virologists Proceedings, 16th Conference, Pgs. 386-391.

Genetic Transformation of Citrus Paradisi with Antisense and untranslatable RNA-dependent RNA Polymerase Genes of Citrus Tristeza Closterovirus - (Peer Reviewed Journal)

Cevik, B., Lee, R.F., Niblett, C.L. 2006. Genetic Transformation of Citrus Paradisi with Antisense and untranslatable RNA-dependent RNA Polymerase Genes of Citrus Tristeza Closterovirus. Turkish Journal of Agriculture and Forestry, 30 (3), Pgs. 173-182.

BIOLOGICAL CHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES - (Proceedings/Symposium) - (04-Jul-05)

BIOCHARCTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES. - (Abstract) - (04-Jul-05)

DWEET MOTTLE VIRUS AND CITRUS LEAF BLOTCH VIRUS - (Popular Publication)

Krueger, R., Lee, R.F., Bash, J.A. 2005. Dweet mottle virus and citrus leaf blotch virus. Subtropics Newsletter V3 No. 1, Pages 7-9.

DWEET MOTTLE VIRUS AND CITRUS LEAF BLOTCH VIRUS IN CALIFORNIA - (Other)

Krueger, R., Bash, J.A., Rangel, B., Lee, R.F. 2005. Dweet mottle virus and citrus leaf virus in california. International Organization of Citrus Virologists Newsletter.

FIRST REPORT OF CITRUS BLIGHT IN COSTA RICA - (Peer Reviewed Journal)

Villalobos, W., Moreira, L., Derrick, K.S., Beretta, J.G., Lee, R.F., Rivera, C. 2005. First report of citrus blight in costa rica. Plant Disease. 89:108

DEVELOPMENT OF STABLE UNIFORM CONTROLS FOR USE IN ELISA ASSAYS FOR CITRUS TRISTEZA VIRUS - (Abstract)

Lee, R.F., Dekkers, M.G., Bar-Joseph, M. 2004. Development of stable uniform controls for use in elisa assays for citrus tristeza virus. 16th Conference International Organization of Citrus Virologists Abstracts, Page 57.

CURRENT RESEARCH ON SPIROPLASMA CITRI IN CALIFORNIA - (Abstract)

Rangel, B., Krueger, R., Lee, R.F. 2007. Current research on spiroplasma citri in california. International Organization of Citrus Virologists Abstracts, Page 88.

PHYTOSANITARY STATUS OF CALIFORNIA CITRUS - (Abstract)

Krueger, R., Bash, J., Lee, R.F. 2007. Phytosanitary status of california citrus. International Organization of Citrus Virologists Abstracts, Page 102.

DEVELOPMENT OF PATHOGEN-DERIVED TRANSGENIC CITRUS FOR RESISTANCE AGAINST CITRUS TRISTEZA VIRUS - (Abstract)

Febres, V.J., Moore, G.A., Lee, R.F. 2004. Development of pathogen-derived transgenic citrus for resistance against citrus tristeza virus. International Organization of Citrus Virologists Abstracts, Page 39.

ECOPORT SLIDE SHOWS ON THE INTERNET RELATED TO CITRUS AND CITRUS DISEASES - (Abstract)

Putter, C.A., Putter, R., Lee, R.F., Roistacher, C.N. 2004. Ecoport slide shows on the internet related to citrus and citrus diseases. International Organization of Citrus Virologists Abstracts, Page107.

DISCRIMINATION OF STEM PITTING CITRUS TRISTEZA VIRUS ISOLATES FROM OTHER CTV ISOLATES - (Abstract)

Sieburth, P.J., Nolan, K.G., Hilf, M.E., Lee, R.F., Moreno, P., Garnsey, S.M. 2004. Discrimination of stem pitting citrus tristeza virus isolates from other ctv isolates. International Organization of Citrus Virologists Abstracts, Page30.

A MULTI-PARTITE RNA VIRUS ASSOCIATED WITH LEPROSIS DISEASE OF CITRUS - (Abstract)

Guerra-Moreno, A.S., Manjunath, K.L., Brlansky, R.H., Lee, R.F. 2004. A multi-partite rna virus associated with leprosis disease of citrus. International Organization of Citrus Virologists Abstracts, Page96.

OCCURRENCE OF CITRUS BLIGHT IN COSTA RICA - (Abstract)

Villalobos, W., Moreira, L., Derrick, K.S., Beretta, M.J., Lee, R.F., Rivera, C. 2004. Occurrence of citrus blight in costa rica. International Organization of Citrus Virologists Abstracts, Page181.

COMPLETE NUCLEOTIDE SEQUENCE OF A NEW GENOTYPE OF CITRUS TRISTEZA VIRUS FROM AN ISOLATE HAVING A MIXED INFECTION - (Abstract)

Manjunath, K.L., Chandrika, R., Warren, C., Niblett, C.L., Lee, R.F. 2004. Complete nucleotide sequence of a new genotype of citrus tristeza virus from an isolate having a mixed infection. International Organization of Citrus Virologists Abstracts, Page 28.

BACTERIAL EXPRESSION AND PURIFICATION OF A PROTEIN FROM CITRUS LEPROSIS VIRUS - (Abstract)

Rangel, E., Manjunath, K.L., Guerra-Moreno, A.S., Brlansky, R.H., Lee, R.F. 2004. Bacterial expression and purification of a protein from citrus leprosis virus. International Organization of Citrus Virologists Abstracts, Page 95.

BIOCHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV) ISOLATES - (Abstract)

Garnsey, S.M., Civerolo, E.L., Gumpf, D.J., Paul, C., Hilf, M.E., Lee, R.F., Brlanskyr, R.H., Yokomi, R.K., Hartung, J.S. 2004. Biocharacterization of an international collection of citrus tristeza virus (CTR) isolates. International Organization of Citrus Virologists Abstracts, Page 49.

A METHOD TO SURVEY FOR CITRUS PATHOGENS WHEN BIOLOGICAL INDICATOR PLANTS ARE NOT AVAILABLE - (Abstract)

Palmieri, M., Donis, I., Salazar, A.L., Cruz, N., Paniagua, A., Brlansky, A., Guerra-Moreno, A.S., Manjunath, K.L., Lee, R.F. 2004. A method to survey for citrus pathogens when biological indicator plants are not available. International Organization of Citrus Virologists Abstracts, Page 105.

LEPROSIS IN GUATEMALA - (Abstract)

Palmieri, M., Donis, I., Salazar, A.L., Blanco, S., Porres, M., Brlansky, R.H., Guerra-Moreno, A.S., Manjunath, K.L., Lee, R.F. 2004. Leprosis in guatemala. International Organization of Citrus Virologists Abstracts, Page 94.

FIRST REPORT OF CITRUS BLIGHT IN COSTA RICA - (Proceedings/Symposium)

Villalobos, W., Moreira, L., Derrick, K.S., Beretta, M.J.G., Lee, R.F., Rivera, C. 2005. First report of citrus blight in costa rica. Plant Disease 89:108.

BIOLOGICAL CHARACTERIZATION OF AN INTERNATIONAL COLLECTION OF CITRUS TRISTEZA VIRUS (CTV)ISOLATES - (Peer Reviewed Journal)

Garnsey, S.M., Civerolo, E.L., Gumpf, D.J., Paul, C., Hilf, M.E., Lee, R.F., Brlansky, R.H., Yokomi, R.K., Hartung, J.S. 2007. Biological characterization of an international collection of citrus tristeza virus (ctv)isolates. In: Proceedings of Conference of International Organization of Citrus Virologists. p. 75-93.

DISTRIBUTION AND CHARACTERIZATION OF CITRUS TRISTEZA VIRUS IN SOUTH FLORIDA FOLLOWING ESTABLISHMENT OF TOXOPTERA CITRICIDA - (Peer Reviewed Journal)

Halbert, S.E., Genc, H., Cevik, B., Brown, L.G., Rosales, I.M., Manjunath, K.L., Pomerinke, M., Davison, D.A., Lee, R.F., Niblett, C.L. 2004. Distribution and characterization of citrus tristeza virus in south florida following establishment of toxoptera citricida. Plant Disease 88:935-941.

SINGLE APHIDS TRANSMIT MULTIPLE GENOTYPES OF CITRUS TRISTEZA VIRUS, BUT OFTEN WITH CHANGED POULATION DYNAMICS - (Abstract)

Biswas, K.K., Manjunath, K.L., Marais, L.J., Lee, R.F. 2004. Single aphids transmit multiple genotypes of citrus tristeza virus, but often with changed poulation dynamics. Phytopathology. 94:S8.

ULTRASTRUCTURAL AND MOLECULAR CHARACTERIZATION OF CITRUS LEPROSIS VIRUS INDICATES POSSIBLE ASSOCIATION OF TWO DISTINCT VIRUSES - (Abstract)

Guerra, A.S., Manjunath, K.L., Brlansky, R.H., Howd, D., Lee, R.F. 2004. Ultrastructural and molecular characterization of citrus leprosis virus indicates possible association of two distinct viruses. Phytopathology. 94:S37.

DEVELOPING RESISTANCE AGAINST CITRUS TRISTEZA VIRUS - (Abstract) - (01-Apr-04)