Projects

IDENTIFICATION, CHARACTERIZATION, AND DETECTION OF FOREIGN AND NEWLY EMERGING DOMESTIC BACTERIA Appropriated (D)   Accession Number: 412092

MOLECULAR DIAGNOSTICS FOR PLANT PATHOGENS Reimbursable (R)   Accession Number: 410363

PCR DETECTION AND CULTIVATION OF HUANGLONGBIN DISEASE AGENT Specific Cooperative Agreement (S)   Accession Number: 409794

CULTIVATION AND RAPID DIAGNOSIS OF THE CAUSAL AGENT OF HUANGLONGBING DISEASE OF CITRUS Trust (T)   Accession Number: 412711

Publications

First report of citrus canker caused by Xanthomonas citri in Somalia - (Peer Reviewed Journal)

Balestra, G.M., Sechler, A.J., Schuenzel, E., Schaad, N.W. 2008. First report of citrus canker caused by Xanthomonas citri in Somalia. Plant Disease. 92:981.

First Report of Crown Gall Caused by Agrobacterium sp. on Diffuse Knapweed (Centaurea diffusa) - (Peer Reviewed Journal)

Eskandari, F., Bruckart, W.L., Schaad, N.W., Sechler, A.J., Postnikova, E.N., Caesar, A.J., Coombs, E. 2008. First Report of Crown Gall Caused by Agrobacterium sp. on Diffuse Knapweed (Centaurea diffusa). Plant Disease. 92:487.

Emerging Plant Pathogenic Bacteria and Global Warming - (Book/Chapter)

Schaad, N.W. 2008. Emerging Plant Pathogenic Bacteria and Global Warming. In: Fatmi, M.B. editor. Pseudomonas syringae Pathovars and Related Pathogens. Dordrecht, The Netherlands: Springer Science + Business Media B.V. 369-379.

A HIGH THROUGHPUT MEMBRANCE BIO-PCR TECHNIQUE FROM ULTRA SENSITIVE DETECTION OF PSEUDOMONAS SYRINGAE PV. PHASEOLICOLA - (Peer Reviewed Journal)

Schaad, N.W., Berthier-Schaad, Y., Knorr, D. 2007. A high throughput membrance bio-pcr technique from ultra sensitive detection of pseudomonas syringae pv. phaseolicola. Plant Pathology. 56:1-8.

Genetic diversity in populations of Xanthomonas campestris pv. camestris in cruciferous weeds in central coastal California - (Peer Reviewed Journal)

Ignatov, A., Sechler, A.J., Schuenzel, E., Agarkova, I.V., Vidaver, A.K., Oliver, B., Schaad, N.W. 2007. Genetic diversity in populations of Xanthomonas campestris pv. camestris in cruciferous weeds in central coastal California. Phytopathology. 97:803-812

GENETIC CHARACTERIZATION AND DIVERSITY OF RATHAYIBACTER TOXICUS - (Peer Reviewed Journal)

Schaad, N.W., Agarkova, I.V., Vidaver, A.K., Postnikova, E.N., Riley, I.T. 2006. Genetic characterization and diversity of rathayibacter toxicus. Phytopathology. 96:1270-1277

GENETIC RELATEDNESS AMOUNG STRAINS OF ACIDOVORAX AVENAE SUBSPECIES AVENAE, CITRULLI AND CATTLEYAE - (Abstract) - (27-Mar-06)

ASSESSMENT OF MULTIPLE DISPLACEMENT AMPLIFICATION FOR INCREASING PCR SENSITIVITY - (Abstract) - (22-Mar-06)

GENETIC DIVERSITY AMONG STRAINS OF ACIDOVORAX AVENAE SUBSP. CITRULLI IN CHINA - (Abstract) - (22-Mar-06)

CHEMICAL CONTROL OF BACTERIAL CANKER IN LIME IN THAILAND - (Abstract) - (22-Mar-06)

GENETIC DIVERSITY IN POPULATIONS OF XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS IN CRUCIFEROUS WEEDS IN CENTRAL COASTAL CALIFORNIA - (Abstract) - (22-Mar-06)

GENETIC DIVERSITY IN POPULATIONS OF XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS IN CRUCIFEROUS WEEDS IN CENTRAL COASTAL CALIFORNIA - (Abstract) - (22-Mar-06)

AN ASSESSMENT MODEL FOR RATING HIGH-THREAT CROP PATHOGENS - (Peer Reviewed Journal)

Schaad, N.W., Abrams, J., Madden, L.V., Frederick, R.D., Luster, D.G., Damsteegt, V.D., Vidaver, A.K. 2006. An assessment model for rating high-threat crop pathogens. Phytopathology 96:616-621

RECLASSIFICATION OF XANTHOMONAS SPECIES PATHOGENIC ON CITRUS - (Peer Reviewed Journal)

Schaad, N.W., Postnikova, E., Lacy, G., Sechler, A.J., Agarkova, I., Stromberg, P., Strombuer, V., Vidaver, A. 2005. Reclassification of xanthomonas species pathogenic on citrus. Systematic and Applied Microbiology. v. 28. p.494-518.

A COMBINED AGAR ABSORBENT AND BIO-PCR ASSAY FOR SENSITIVE DETECTION OF XYLELLA FASTIDIOSA IN GRAPE AND CITRUS - (Peer Reviewed Journal)

Fatmi, M., Damsteegt, V.D., Schaad, N.W. 2005. A combined agar absorbent and bio-pcr assay for sensitive detection of xylella fastidiosa in grape and citrus. Plant Pathology. 54:1-7

GENETIC DIVERSITY AMONG CLAVIBACTER MICHIGANENSIS SUBSP. MICHIGANENSIS ND SUBSP. SEPEDONICUS IN RUSSIAN FEDERATION - (Abstract)

Phytopathology 94:543

IDENTIFICATION OF RATHAYIBACTER RATHAYI IN OREGON AND MARYLAND - (Abstract)

Phytopathology 94:S85-86

GENETIC DIVERSITY AMONG BACTERIA ASSOCIATED WITH BROWN ROT OF POTATO IN RUSSIAN FEDERATION - (Abstract)

Phytopathology 94:S67

NEW ASSOCIATION OF A TOXIGENIC RATHAYIBACTER SP. AND ANGUINA WOODI IN EHRHARTA VILLOSA VAR. VILLOSA IN SOUTH AFRICA - (Abstract)

Phytopathology 94:S88

RECLASSIFICATION OF THE XANTHOMONADS ASSOCIATED WITH BACTERIAL SPOT DISEASE OF TOMATO AND PEPPER - (Peer Reviewed Journal)

System. Appl. Microbiology 27:755-762

IDENTIFICATION OF RATHAYIBACTER RATHAYI IN OREGON AND MARYLAND - (Abstract)

Postnikova, E., Agarkova, I., Riley, I.T., Alderman, S.C., Ocamb, C.M., Vidaver, A.K., Schaad, N.W. 2004. Identification of rathayibacter rathayi in oregon and maryland. Phytopathology Supplement; APSnet (Plant Pathology Online). v. 94(Supplement)S85.

REAL-TIME PCR ASSAYS FOR DETECTING BACTERIA IN SEED AND OTHER PLANTING MATERIAL - (Abstract)

International Plant Protection Congress Abstracts XV:63

DEVELOPMENT OF ASSESSMENT MODELS FOR RATING HIGH-THREAT CROP PATHOGENS - (Abstract)

Schaad, N.W., Abrams, J., Madden, L.V., Frederick, R.D., Luster, D.G., Damsteegt, V.D., Vidaver, A.K. 2004. Development of assessment models for rating high-threat crop pathogens. American Society for Microbiology. 215:72.

USE OF A COMPLETE DRY BEAD FORMULATION FOR MORE SIMPLIFIED RELIABLE REAL-TIME PCR DETECTION OF BACTERIA - (Abstract)

Schaad, N.W., Gaush, P.E., McMillan, W. 2004. Use of a complete dry bead formulation for more simplified reliable real-time PCR detection of bacteria. American Society for Microbiology. 67:72.

DESCRIPTION OF NEW SUBSPECIES OF XYLELLA FASTIDIOSA SUBSP. PIERCEI, SUBSP. NOV., X. FASTIDIOSA SUBSP. AGGLOMERII SUBSP. NOV., AND X FASTIDIOSA SUBSP. IDIAOTRAPOSA SUBSP. NOV. - (Peer Reviewed Journal)

Schaad, N.W., Postnikova, E., Fatmi, M., Lacy, G., Chang, C. 2004. Description of new subspecies of xylella fastidiosa subsp. piercei, subsp. nov., x. fastidiosa subsp. agglomerii subsp. nov., and x fastidiosa subsp. idiaotraposa subsp. nov.. Systematic and Applied Microbiology.27:290-300.

GENETIC ANALYSIS OF RALSTONIA SOLANACEARUM STRAINS FROM DIFFERENT HOSTS IN THAILAND USING PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM - (Peer Reviewed Journal)

Thammakijjawat, P., Thaveechai, N., Kositratana, W., Chunwongse, J., Frederick, R.D., Schaad, N.W. 2004. Genetic analysis of Ralstonia solanacearum strains from different hosts in Thailand using PCR-restriction fragment length polymorphism. Kasetsart Journal. 35:397-408.

AMPLI-DISK PCR, A NOVEL TECHNIQUE FOR ULTRA-SENSITIVE DETECTION OF BACTERIA - (Other)

Schaad, N.W., Randhawa, P. 2003. Ampli-disk PCR, a novel technique for ultra-sensitive detection of bacteria. Patent Application. DN 0110.03, SN 10/689,624.

ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES - (Review Article)

SCHAAD, N.W., FREDERICK, R.D., SHAW, J., SCHNEIDER, W.L., HICKSON, R., PETRILLO, M.D., LUSTER, D.G. ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES. ANNUAL REVIEW OF PHYTOPATHOLOGY. 41: . 2003.

EVALUATION OF A ON-SITE, ONE-HOUR REAL-TIME PCR ASSAY FOR DETECTING THE CITRUS CANKER BACTERIUM IN PLANT SAMPLES AT PORT FACILITIES - (Abstract)

Schaad, N.W., Opgenorth, D., Petrillo, M.D., Abdelshife, M.A., Tedla, T. 2003. Evaluation of a on-site, one-hour real-time pcr assay for detecting the citrus canker bacterium in plant samples at port facilities. Phytopathology. 92:S76.

XYLELLA FASTIDIOSA TAXONOMY - (Abstract)

SCHAAD, N.W., POSTNIKOVA, E., FATMI, M., LACY, G., CHANG, C.J. XYLELLA FASTIDIOSA TAXONOMY. Phytopathology. 93:S76. 2003.

A COMBINED AGAR ABSORBENT AND BIO-PCR ASSAY FOR RAPID, SENSITIVE DETECTION OF XYLELLA FASTIDIOSA (XF) IN GRAPE AND CITRUS - (Abstract)

Fatmi, M., Damsteegt, V.D., Schaad, N.W. 2003. A combined agar absorbent and BIO-PCR assay for rapid, sensitive detection of Xylella fastidiosa (XF) in grape and citrus. Phytopathology. 93:S25.

DEVELOPMENT OF A REAL-TIME PCR ASSAY TO DETECT RALSTONIA SOLANACEARUM RACE 3, BIOVAR 2 (BV2) IN GERANIUM PLANTS - (Abstract)

Nikolaeva, E., Matveeva, E., Schaad, N.W. 2003. Development of a real-time PCR assay to detect ralstonia solanacearum race 3, biovar 2 (BV2) in geranium plants. Phytopathology. 93:S65

COMPARISON BETWEEN ISOLATION AND PCR FOR DETECTION OF XANTHOMONAS CITRI IN NEW LESIONS ON IMMATURE CITRUS LEAVES AND OLD LESIONS ON MATURE CITRUS LEAVES IN THAILAND - (Abstract)

Schaad, N.W., Postnikova, E., Fatmi, M., Lacy, G. Chang, C.J. 2003. Xylessa fastidiosa taxonomy. Phytopathology. 93:S76.

THE USE OF ACIDIC ELECTROLYZED WATER TO ELIMINATE MICROORGANISMS FROM WHEAT SEED OR SOIL TO DETECT TILLETIA INDICA TELIOSPORES - (Peer Reviewed Journal)

Bonde, M.R., Nester, S.E., Schaad, N.W., Frederick, R.D., and Luster, D.G. Improved detection of Tilletia indica teliospores in seed or soil by elimination of contaminating microorganisms with acidic electrolylized water. PLANT DISEASE. 87:712-718. 2003.

USE OF A SEED SCARIFIER FOR DETECTION AND ENUMERATION OF GALLS OF ANGUINA AND RATHAYIBACTER SPECIES IN ORCHARD GRASS SEED - (Peer Reviewed Journal)

ALDERMAN, S.C., BILSLAND, D.M., GRIESBACH, J.A., MILBRATH, G.M., SCHAAD, N.W., POSTNIKOVA, E. USE OF A SEED SCARIFIER FOR DETECTION AND ENUMERATION OF GALLS OF ANGUINA AND RATHAYIBACTER SPECIES IN ORCHARD GRASS SEED. PLANT DISEASE. 2003. v.87. p. 375-379.

GENETIC VARIABILITY OF RALSTONIA SOLANACEARUM STRAINS FROM PEPPER (CAPSICUM ANNUUM) IN THAILAND AND THEIR GENETIC RELATIONSHIP TO STRAINS FROM OTHER HOSTS - (Peer Reviewed Journal)

Thammakijjawat, P., Thaveechai, N., Kositratana, W., Chumwongse, J., Frederick, R.D., Schaad, N.W. 2004. Genetic variability of Ralstonia solanacearum strains from pepper (Capsicum annuum) in Thailand and their genetic relationship to strains from other hosts. Thai Journal of Agricultural Science. 35:397-408.

USE OF PORTABLE REAL-TIME PCR FOR SAME-DAY ON-SITE FIELD DIAGNOSIS OF BACTERIAL DISEASES. - (Abstract)

SCHAAD, N.W., GAUSH, P.E., POSTNIKOVA, E. USE OF PORTABLE REAL-TIME PCR FOR SAME-DAY ON-SITE FIELD DIAGNOSIS OF BACTERIAL DISEASES. Phytopathology. 92:S72.

PHYLOGENETIC STUDIES OF CORN AND RICE STRAINS OF ACIDOVORAX AVENAE SUBSP. AVENAE BY DNA/DNA HYBRIDIZATION - (Abstract)

POSTNIKOVA, E., SCHAAD, N.W. PHYLOGENETIC STUDIES OF CORN AND RICE STRAINS OF ACIDOVORAX AVENAE SUBSP. AVENAE BY DNA/DNA HYBRIDIZATION. Phytopathology. 92:S66. 2002.

RALSTONIA SOLANACEARUM BIOVAR 2, RACE 3 IN GERANIUM IMPORTED FROM GUATEMALA TO PENNSYLVANIA IN 1999 - (Abstract)

KIM, S.H., OLSON, T.N., SCHAAD, N.W. RALSTONIA SOLANACEARUM BIOVAR 2, RACE 3 IN GERANIUM IMPORTED FROM GUATEMALA TO PENNSYLVANIA IN 1999. PHYTOPATHOLOGY. 92:S42. 2002.

BACTERIAL PATHOGENS ON CEREALS IN THE RUSSIAN FEDERATION - (Abstract)

MATVEEVA, E.V., POLITYKO, V.A., IGNATOV, A.N., NIKOLAEVA, E.V., PEKHTEREVA, E.S., SCHAAD, N.W. BACTERIAL PATHOGENS ON CEREALS IN THE RUSSIAN FEDERATION. PHYTOPATHOLOGY. 92:S52. 2002.