Project Number: 5320-21000-011-08
Project Type: Trust

Start Date: Jan 01, 2007
End Date: Dec 31, 2011

Objective:
1-To complete southern analysis of transgenic papaya. 2-To determine border nucleic acid sequences of the transgene inserts of the transgenic papaya. 3-To assemble data and initiate in deregulation package for Japan.

Approach:
DNA of transgenic SunUp and Rainbow papaya will be isolated and subjected to southern blot analysis using probes developed from the transformation vector. This will tell us the number of transgene inserts. Then PCR and sequencing experiments will be done to obtain the nucleic acid border sequences of each insert. The results will be compiled and written into a document to deregulate the transgenic papaya in Japan. Documents Trust with the HI Papaya Industry Association. Log 32314.