Project Number: 5442-22000-043-02
Project Type: Reimbursable

Start Date: Sep 01, 2005
End Date: Dec 31, 2008

Objective:
In this project we will assess the feasibility and efficiency of TRAP markers for rapidly mapping the barley genome using the Rika x Kombar doubled haploid population. Using the TRAP markers in combination with chromosome specific microsatellite markers to identify the chromosomal locations of genes and/or QTL associated with resistance to P. teres in the Rika x Kombar doubled haploid population.

Approach:
The barley doubled haploid population Rika X Kombar (RK) was developed for this project to use disease resistance traits to assess the effectiveness of the target region amplified polymorphism (TRAP) technique for use in rapidly mapping the barley genome. The RK population segregates for resistance to two pathotypes of Pyrenophora teres f. teres and at least one pathotypes of P. teres f. maculata. The genes associated with resistance to these P. teres pathotypes will be mapped using TRAP markers followed by the use of simple sequence repeat (SSR) and restriction fragment length polymorphism (RFLP) markers for the anchoring of chromosome arms containing these genes. This project will use disease resistance traits to show the usefulness of the TRAP technique in efficiently mapping traits in barley.