Volume 10, Number 4, April 2004

Pneumocystis jirovecii Dihydropteroate Synthase Genotypes in Immunocompetent Infants and Immunosuppressed Adults, Amiens, France

Anne Totet,* Sophie Latouche,† Philippe Lacube,† Jean-Claude Pautard,* Vincent Jounieaux,* Christian Raccurt,* Patricia Roux,† and Gilles Nevez*
*University Hospital, University of Picardy, Amiens, France; and †Saint-Antoine University Hospital, Paris, France

Figure 1. Identification of Pneumocystis jirovecii dihydropteroate synthase (DHPS) genotypes. Electrophoresis on 1.5% agarose gel of DHPS polymerase chain reaction products after digestion with AccI (upper line) and HaeIII (lower line). M, molecular weight marker (123 Gibco BRL; Cergy Pontoise, France). Lane 1: double mutant genotype; lanes 2 and 5: wild genotype; lane 3: mixed infection with a wild genotype and a mutant genotype that has the mutation at nucleotide position 165; lane 4: mixed infection with a wild genotype and a mutant genotype which has the mutation at nucleotide position 171.

EID Home | Top of Page | Ahead-of-Print | Past Issues | Suggested Citation | EID Search | Contact Us | Accessibility | Privacy Policy Notice | CDC Home | CDC Search | Health Topics A-Z

This page last reviewed March 16, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention