Volume 10, Number 12, December 2004

Isolation and Molecular Identification of Nipah Virus from Pigs

Sazaly AbuBakar,* Li-Yen Chang,* A.R. Mohd Ali,† S.H. Sharifah,† Khatijah Yusoff,‡ and Zulkeflie Zamrod§
*University of Malaya, Kuala Lumpur, Malaysia; †Veterinary Research Institute, Ipoh, Perak, Malaysia; ‡Universiti Putra Malaysia, Selangor, Malaysia; and §Universiti Kebangsaan Malaysia, Selangor, Malaysia

Figure. Phylogenetic trees illustrating the relationships of the pig Nipah virus isolates to all other known Nipah viruses and the related members of subfamily Paramyxovirinae. A) The maximum likelihood tree was drawn by using alignments of the full genome sequences. All the new isolates described in the study are shown in boxes. Abbreviations used and accession numbers not described elsewhere in the text are in parenthesis: Tupaia paramyxovirus (Tupaia-A) (AF079780); (Tupaia-N) (NC_002199), and Hendra virus (HV-A) (AF017149); (HV-N) (NC_001906). B) Unrooted maximum likelihood plot was constructed by using alignments of all the nucleotide differences in the Nipah virus gene coding regions (N, P, M, F, and G) shown in the Appendix by artificially treating all the differences as a single stretch of nucleotide sequence.

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This page last reviewed November 12, 2004

Emerging Infectious Diseases Journal
National Center for Infectious Diseases
Centers for Disease Control and Prevention