ARS | Publication request: APPLICATION OF MICROSATELLITE AND RAPD FINGERPRINTS IN THE FLORIDA SUGARCANE VARIETY PROGRAM

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract
Publication Acceptance Date: November 20, 2002
Publication Date: January 20, 2003
Citation: Pan, Y., Miller, J.D., Schnell Ii, R.J., Richard Jr, E.P., Wei, Q. 2003. Application of microsatellite and RAPD fingerprints in the Florida sugarcane variety program [abstract]. Plant and Animal Genome Abstract XI Abstract Book. W 189. p. 43

Technical Abstract: Twenty-five Florida sugarcane varieties were fingerprinted with three microsatellites, namely, SMC334BS, SMC336BS, and MCSA068G08, using a capillary electrophoresis system. Multiple alleles were detected from each microsatellite that exhibited a high level of polymorphism. There were six alleles for SMC334BS, five for SMC336BS, and seven for MCSA068G08 with a total of 18 alleles. A putative genotype is assigned to each variety by an arbitrary nucleotide sequence of A (if a particular allele is present) or C (if the same allele is absent). The genetic similarity among these varieties was assessed with a DNA sequence analysis software, DNAMAN®, to produce a pairwise homology matrix and a homology tree. Only two pairs of varieties, CP 70-1133/CP 92-1213 and CP 81-1384/CP 98-1462, shared an identical genotype. The 25 varieties were clustered into three groups, with genetic similarities greater than 70% with each group. Group I included CP 98-1462, CP 81-1384, CP 92-1666, CP 98-1119, CP 92-1641, CP 72-2086, CP 89-2143, CP 96-1602, CP 81-1254, and CP 94-1340. Group II included CP 92-1213, CP 70-1133, CP 73-1547, CP 93-1309, CP 86-1633, CP 82-1592, CP 72-1210, CP 94-1100, CP 98-1840, CP 95-1039, CP 88-1762, CP 80-1743, and CP 94-1591. Group III included CP 85-1308 and CP 84-1198. The availability of microsatellite fingerprints allowed the sugarcane breeders to identify and discard two mis-labeled sugarcane varieties from field trials that also produced distinct RAPD fingerprints. In addition, the microsatellite fingerprints helped sugarcane breeders evaluate the efficiency of the current conventional breeding methods and clarify the genetic pedigree of commercial sugarcane varieties.