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Patent Title: A Novel System for Linking Several DNA Sequences
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BT Patent Number: 6372479
Docket Number: 21400
Serial Number: 9656062
Date Patented: 04/16/2002
Technology Description:
Agricultural Research Service scientists have developed a novel system and method for the sequential, directional cloning of multiple DNA sequences into a single vector. It is a facile process by which multiple genes, sufficient for the expression and/or regulation of a metabolic pathway, can be simultaneously introduced into the target species in one transformation event. The process uses designed primers (added to DNA sequences via PCR or ligation) which contain sequences that generate compatible cohesive ends upon digestion by the appropriate restriction enzymes. The method ensures the directionality of the cloned sequences, and permits the insertion of additional DNA sequences into multigene arrays previously constructed by this process. To demonstrate its utility, the invention method was used to construct three and fourgene arrays that were introduced into Fusarium sporotrichioides. These arrays enabled the biosynthesis of the carotenoids lycopene and betacarotene, which are utilized as colorants and nutritional supplements. By constructing multigene arrays containing different metabolic enzymes, F. sporotrichioides strains capable of synthesizing other products such as rubber or taxol could be developed. The invention could potentially be used to facilitate the introduction of novel metabolic pathways into any number of fungal or plant species, thereby expanding the productivity and /or economic value of the species.
The technology applies to biotechnology companies that produce DNA cloning kits. Pharmaceutical companies that produce vitamins and medicines can also use this technology. Agricultural companies interested in developing new crop varieties would also benefit.
Reference:
Please refer to USPN 6,372,479 (Docket #0214.00), "Fusarium Sporotrichioides for Production of B-Carotene," which issued April 16, 2002, and is a divisional of USPN 6,184,000 (Docket #0259.97), which issued February 6, 2001.
Inventors:
James D. Jones 2542 W. Fitch, Second Floor Chicago, IL 60645 (773) 3810619 (Formerly with Fermentation Biotechnology Research Peoria) |
Thomas M. Hohn 102 Blakely Drive Chapel Hill, NC 27514 (919) 9684225 |
Timothy D. Leathers Fermentation Biotechnology Resarch Peoria, IL 616043999 (309) 6816377 / Fax: (309) 68166427 |
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Last Modified: 10/16/2008
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