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Field evaluation of the gag-based heteroduplex mobility assay for genetic subtyping of circulating recombinant forms of human immunodeficiency virus type 1 in Abidjan, Cote d'Ivoire.
Journal of Clinical Microbiology 2003; 41(7):3056-3059.
Sawadogo S, Adjé-Touré C, Bilé CE, Ekpini EA,
Chorba T, Nkengasong JN.
Abstract
The gag-based heteroduplex mobility assay (gag-HMA) was evaluated for its ease
and reliability in subtyping circulating recombinant forms (CRFs) of human
immunodeficiency virus type 1 (HIV-1) in Cote d'Ivoire. One hundred thirty-two
plasma samples were analyzed blindly for HIV-1 subtypes by sequencing the
pol gene and by gag-HMA. DNA sequencing was used as the "gold standard." Of
the 132 samples sequenced, 108 (82%) were CRF02_AG, 14 (11%) were pure subtype
A, 5 (4%) were subtype G, 3 (2%) were subtype D, 1 was CRF01_AE, and 1 was
subtype H. The gag-HMA correctly classified 126 (95.5%) of the samples. Of
the 108 samples that were classified as CRF02_AG by DNA sequencing, 107 (99%)
were correctly identified by gag-HMA, resulting in a positive predictive
value of 96.4%. The gag-HMA seems to be a valuable tool for understanding
the molecular epidemiology of HIV-1 CRF02_AG in Cote d'Ivoire and West Africa,
which could be important for developing and evaluating AIDS vaccines, although
DNA sequencing remains necessary for accurate molecular epidemiology.