Cardona NM, Revankar SG, Ortiz P, Kirkpatrick WR, McAtee R, Patterson TF; American Society for Microbiology. General Meeting.

Abstr Gen Meet Am Soc Microbiol. 1997 May 4-8; 97: 261 (abstract no. F8).

Laboratorio de Microbiologia, Instituto Colombiano de Medicina Tropical, Medellin, Colombia.

Forty healthy Colombian women (ages 18-45) with vulvo-vaginal candidiasis (VC) were enrolled in a study to receive either fluconazole or itraconazole. Isolates from 10 patients with recurrent disease were selected for analysis by molecular techniques. Forty nine (49) Candida spp isolates were examined (19 C. albicans, 7 C. tropicalis, 21 C. krusei and 2 C. glabrata). One isolate was taken from each symptomatic episode: patient (Pt) 1 had C. albicans from the first episode and C. tropicalis from the other 7 episodes; Pt 2 had C. krusei from 4 episodes; Pt 3 had C. albicans from 3 episodes; Pt 4 had C. albicans from 4 episodes; Pt 5 had C. krusei from 4 episodes; Pt 6 had C. albicans from 3 episodes, Pt 7 had C. albicans from 4 episodes, C. glabrata from the 5th episode, and C. krusei from the last 3 episodes; Pt 8 had C. krusei from 6 episodes; Pt 9 had C. albicans from the first 4 episodes and C. glabrata from the 5th episode, Pt 10 had C. krusei from 4 episodes. Pulsed Field Gel Electrophoresis (PFGE) of whole-cell DNA and Restriction Fragment Length Polymorphisms (RFLP) generated from whole-cell DNA digested with the restriction endonuclease SfiI were performed with the 49 isolates to determine strain karyotype identity. PFGE and RFLP of the Candida isolates demonstrated that 4 patients had relapses by the same strain, 3 patients had infection by the same species but different strain and 3 patients had relapses with the same or different strains of multiple species. This work suggests that in this population, recurrent VC may often be due to non-albicans species, notably, C. krusei. Recurrences may be due to the same or different species and/or strains. Both techniques can be a useful in evaluating the etiology of recurrent VC.

Publication Types:

• Meeting Abstracts

Keywords:

• Candida
• Candidiasis
• Candidiasis, Vulvovaginal
• Chromosomes
• Chromosomes, Fungal
• Electrophoresis, Gel, Pulsed-Field
• Female
• Fluconazole
• Humans
• Itraconazole
• Karyotyping
• Polymorphism, Restriction Fragment Length
• Recurrence
• genetics

Other ID:

• 98928672

UI: 102235325

From Meeting Abstracts