Friedman-Kien AE, Saltzman BR, Cao YZ, Mirabile M, Li JJ, Nestor M, Peterman TA; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 214 (abstract no. S.B.512).
New York University Medical Center, NY, NY, USA
OBJECTIVE: To present 7 cases of Kaposi's Sarcoma (KS) in HIV-1 seronegative homosexual men. METHODS: 350 serum samples from sexually active homosexual male patients with biopsy proven KS were tested for antibodies to HIV-1 by ELISA and Western Blot (WB) techniques. 286 of the 350 serum samples were also tested by using the HIV-1 p24 antigen capture assay. Detection of HIV-1 DNA sequences in the peripheral blood mononuclear cells (PBMC) from the HIV-1 seronegative KS patients were performed using a polymerase chain reaction (PCR) test with HIV-1 gag primers. The presence of HIV-1 neutralizing activity (NT act.) was determined by using a neutralization assay performed with the H9 clones as target cells and HTLV-RF as the virus inoculum in the HIV-1 seronegative samples. RESULTS: 343 of the 350 samples (98.0%) from these patients with KS were positive for antibodies to HIV-1. 106 of 279 HIV-1 seropositive samples (37.9%) were positive for HIV-1 p24 antigen. 7 of the 350 samples (2%) tested were HIV-1 seronegative. All 7 of these individuals had normal absolute numbers of CD4 T-helper cells. The PBMC samples available from 6 of the 7 HIV-1 seronegative patients with KS were HIV-1 DNA/PCR negative. HIV-1 serum NT act. was negative in all 7 HIV-1 seronegative samples. CONCLUSION: These findings suggest that KS occurring in homosexual men may possibly be caused by yet another sexually transmitted infectious agent other than HIV-1, perhaps more readily expressed in the setting of HIV-1 infection.
Publication Types:
Keywords:
- Enzyme-Linked Immunosorbent Assay
- Genes, gag
- HIV Infections
- HIV Seropositivity
- HIV-1
- Homosexuality
- Homosexuality, Male
- Humans
- Male
- Polymerase Chain Reaction
- Sarcoma, Kaposi
- genetics
Other ID:
UI: 102196331
From Meeting Abstracts