Tsai C, Tsai C, Sligh J, Woon M, Roodman S; International Conference on AIDS.
Int Conf AIDS. 1989 Jun 4-9; 5: 600 (abstract no. W.C.P.52).
St. Louis University, St. Louis, Missouri, USA
OBJECTIVE: To establish KS-like cell lines from SAIDS associated with SRV-2. METHODS: Two KS-like lesions, subcutaneous and retroperitoneal fibromatosis from macaques with SAIDS associated with SRV-2 infection were cultured in regular RPMI or DMEM media supplemented initially with brain derived growth factor (BDGF). Immunocytochemical, cytologic, ultrastructural and viral studies were carried out on various passages of culture (over 16) during a 12 months period. RESULTS: Both cell lines propagate vigorously in 10% FCS media without BDGF after 4th passage and can grow with only 0.5% FCS unlike the control monkey fibroblast. Cytology and immunochemistry of both cells are similar and demonstrated heterogeneous populations of endothelial (+/-60%), pericytic/ smooth muscle (+/-15%), and primitive mesenchymal/fibroblastic differentiations. Arborizing multinuclear syncytial giant cells are prominent in early culture. Both cell lines are diploid (DI=1.02) with high proliferative activity (S=25% G2/M=30%) but show no growth in soft agar. Evidence of autocrine/paracrine growth is further suggested by the presence of cytoplasmic phosphotyrosine and BDGF-like proteins in these cultured cells. CONCLUSION: Two KS-like cell lines from simian AIDS associated with SRV-2 were established for future basic and clinical study.
Publication Types:
Keywords:
- Animals
- Betaretrovirus
- Cell Differentiation
- Cell Line
- Communicable Diseases
- Fibromatosis, Abdominal
- Flow Cytometry
- Infection
- Macaca
- Mason-Pfizer monkey virus
- Sarcoma, Kaposi
- Simian Acquired Immunodeficiency Syndrome
Other ID:
UI: 102179061
From Meeting Abstracts