Construction of a Recombinant Mammalian Expression System for the Production of Human TGF-beta 1 and Members of TGF-beta Superfamily Cytokines
Description of Invention:
Transforming growth factor-beta 1 (“TGF-beta 1”) is an anti-inflammatory cytokine and is widely used in immunological research. Various recombinant expression systems produce TGF-beta 1, however, the yield of such expression systems remains low with the most effective systems producing from 1-5 mg/liter of cell culture with lengthy purification steps. As a result, the availability and price of the cytokine is unsatisfactory.
To address this problem, this invention provides a novel mammalian recombinant TGF-beta expression system which produces TGF-beta 1 at approximately 30 mg/liter of cell culture, which is approximately 10 times better than the yield provided by existing recombinant TGF-beta 1 expression systems. Owing to the large superfamily of cytokines to which TGF-beta belongs, this expression system can be potentially applied to other members of the TGF-beta superfamily.
Inventors:
Zhongcheng Zou and Peter Sun (NIAID)
Patent Status:
DHHS Reference No. E-048-2004/0 --
U.S. Provisional Application No. 60/534,379 filed 06 Jan 2004
DHHS Reference No. E-048-2004/1 --
U.S. Provisional Application No. 60/575,849 filed 02 Jun 2004
DHHS Reference No. E-048-2004/2 --
PCT Application No. PCT/US2005/00378 filed 06 Jan 2005, which published as WO 2005/068499 on 28 Jul 2005
U.S. Patent Application No. 10/585,499 filed 06 Jul 2006
For Additional Information Please Contact: David A. Lambertson Ph.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301)435-4632
Email: lambertsond@mail.nih.gov
Fax: (301) 402-0220
