Construction of an Infectious Full-Length cDNA Clone of the Porcine Enteric Calicivirus RNA Genome
Description of Invention:
Porcine enteric calicivirus (PEC) is a member of the genus Sapovirus in the family Caliciviridae. This virus causes diarrheal illness in pigs, and is presently the only enteric calicivirus that can be grown in cell culture. In addition to its relevance to veterinary medicine as a diarrheal agent in pigs, PEC serves as an important model for the study of enteric caliciviruses that cause diarrhea and that cannot be grown in cell culture (including the noroviruses represented by Norwalk virus). The development of an infectious cDNA clone is important because it enables the use of “reverse genetics” to engineer mutations of interest into the genome of PEC and to study their effects. In addition, it allows the introduction of foreign coding sequences into the genome of PEC that could be useful for vaccine development in swine and possibly humans. This discovery has both basic research applications such as mapping mutations involved in tissue culture adaptation, tissue tropism, and virulence as well as practical applications such as providing a genetic backbone for the development of chimeric vaccine viruses.
Inventors:
Kyeong-Ok Chang (NIAID) Stanislav V. Sosnovtsev (NIAID) Gael M. Belliot (NIAID) Kim Y. Green (NIAID) et al.
Patent Status:
DHHS Reference No. E-214-2003/0 -- Research Material
Related Technologies:
DHHS Reference No. E-198-2003/0 -- Research Material
Construction of Recombinant Baculoviruses Carrying the Gene Encoding the Major Capsid Protein, VP1, from Calicivirus Strains (including Norovirus Strains Toronto, Hawaii, Desert Shield, Snow Mountain, and MD145-12)
DHHS Reference No. E-212-2003/0 – Research Material
Full-Length cDNA Clone Representing the Consensus Sequence of the RNA Genome of a Human Norovirus (strain MD145-12) that Encodes Biologically Active Proteins
DHHS Reference No. E-283-2003/0 -- Research Material
Enzymatically-Active RNA-Dependent RNA Polymerase From a Human Norovirus (Calicivirus)
Relevant Publication: The materials are further described in KO Chang et al., "Cell-culture propagation of porcine enteric calicivirus mediated by intestinal contents is dependent on the cyclic AMP signaling pathway," Virology. 2002 Dec 20;304(2):302-310. [PubMed abs]
Licensing Status: The materials embodied in this invention are available nonexclusively through a biological materials license.
Collaborative Research Opportunity:
The Laboratory of Infectious Diseases, NIAID, NIH, is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize reagents derived from an infectious cDNA copy of the genome of porcine enteric calicivirus. Please contact Kim Y. Green at kgreen@niaid.nih.gov for more information.
For Additional Information Please Contact: Peter A. Soukas J.D.
NIH Office of Technology Transfer
6011 Executive Blvd, Suite 325
Rockville, MD 20852-3804
Phone: (301) 435-4646
Email: soukasp@mail.nih.gov
Fax: (301) 402-0220
