Electron Microscopy Unit
The Electron Microscopy (EM) Unit is dedicated to providing state-of-the-art technologies to support the ultra-structural imaging needs of Division of Intramural Research scientists both in Bethesda, MD, and at the Rocky Mountain Laboratories in Hamilton, MT. The facility provides sample preparation and analysis ranging from basic structural studies to immuno-localization of selected antigens for a wide array of specimens. A variety of methods, protocols, and equipment are employed to accommodate different preparative and imaging needs. Real-time viewing of samples by video image-sharing is available to NIAID users, enabling scientists on the main campus to view samples simultaneously with microscopy staff.
Available Technologies
Electron Microscopes:
- FEI CM-10 120 kV transmission electron microscope
- Hitachi H-7500 transmission electron microscope
- Hitachi S-4500 scanning electron microscope with Gatan Alto 2500 cryo-stage
- Hitachi S-5200 high resolution in-lens scanning electron microscope with Gatan 626 cryo-stage
- FEI Tecnai 12 transmission electron microscope with tomography package (Coming in summer 2008)
- FEI Titan Krios 300kV transmission electron microscope with tomography package (Coming in fall 2009)
Advanced Preparative Technologies:
- Leica UCT cryo-ultramicrotome
- Leica EMPact high pressure freezer
- Leica automated freeze substitution device
- Ted Pella microwave oven
- EMS Lynx automated tissue processor
- BAF 060 high resolution cryo-sputter coater
- RMC MT-7000 ultramicrotomes
Confocal Microscope:
- Zeiss 510 meta confocal microscope with Coherent Chameleon Ultra II multiphoton detector and incubation system
Microscopists
Beth Fischer: 406-363-9378
Dave Dorward: 406-363-9266
Vinod Nair: 406-363-9254
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