Project Number: 1275-22000-230-15
Project Type: Reimbursable

Start Date: Aug 01, 2006
End Date: Sep 30, 2008

Objective:
To discover gene sequences that can be used to distinguish Planococcus citri and P. minor. Preliminary results suggest that segments of the mitochondrial gene COI and the nuclear gene EF1-' will be useful in separating them. We will examine Rapid PCR and other technologies to develop a diagnostic tool that will be useful for an efficient and effective method for separation of the species. We also will examine morphological features to determine if structural characters exist that could be used to make accurate determinations.

Approach:
Gather specimens from as many localities as possible. Extract DNA and mount exoskeleton as a voucher and representative for morphological analysis. PCR and sequence extracted DNA. Align sequences using computer algorithms and use data to formulate phylogenetic trees. Preliminary data suggest that segments of EF1-' and COI are useful in distinguishing these species. Utilize unique base pairs from each of Planococcus citri and P. minor to develop a diagnostic tool that will quickly distinguish these species. Examine exoskeletons of molecularly identified specimens to determine if structural characters can be utilized to distinguish these mealybugs.