Project Number: 6402-21220-007-01
Project Type: Specific Cooperative Agreement

Start Date: May 10, 2001
End Date: May 09, 2006

Objective:
The objective is to evaluate the resistance of Public and Private soybean varieties and breeding lines to the most serious diseases present in the southern United States.

Approach:
The Delta Research and Extension Center, Mississippi Agricultural and Forestry Experiment Station, Stoneville, Mississippi, will obtain seed from the entries in the Mississippi Soybean State Variety Trials, which averages 205 entries in Maturity Groups IV-VI. Additional seed of Maturity Group III and breeding lines from the USDA soybean breeder at Stoneville will also be obtained. These varieties and breeding lines will be screened for resistance to the following diseases: 1. Stem Canker 2. Pod and Stem Canker. The Toothpick method of inoculation will be used to evaluate resistance to these diseases. 3. Frogeye Leaf Spot. Spores from selected races which are prevalent in Mississippi will be sprayed on the soybeans. 4. Phytophthora root rot. Hypocotyl and/or layering techniques will be used to evaluate resistance and field tolerance to the prevalent races in Mississippi, which are 2, 4, 7, and 10. 5. Soybean Mosaic Virus resistance will be determined by using an airbrish to inoculate the soybeans and by ELISA and blot tests.