Project Number: 1910-21000-019-06
Project Type: Specific Cooperative Agreement

Start Date: Sep 29, 2006
End Date: Dec 31, 2009

Objective:
The objective of this cooperative research project is to screen the core collections of Lycopersicon wild species and four related Solanum spp. maintained in the National Plant Germplasm System (NPGS) for resistance to bacterial speck race 1. If resistance is not found in the core collections, all additional wild species Lycopersicon accessions maintained in the NPGS (an additional 400 accessions) will be screened for resistance to bacterial speck race 1.

Approach:
The core collections for wild species of Lycopersicon (212 accessions) and four related Solanum spp (20 accessions) will be screened for bacterial speck race 1 resistance. After this, the rest of the wild species accessions of tomato (an additional 400 accessions) will be screened for bacterial speck race 1 resistance. These will be grown in 12 replicates with 3 plants per replicate in the greenhouse and will be inoculated with bacterial speck race 1 and then maintained at 100% humidity in the greenhouse at 250C for one week. The plants then will be maintained in a greenhouse for 2 weeks when they will then be scored for lesion development on a reaction scale. Experiments will always include uninoculated controls. All experiments will be repeated at least once and if resistance is suspected, there will be more repeats.