Project Number: 5348-21000-024-09
Project Type: Trust

Start Date: Dec 20, 2005
End Date: Dec 31, 2009

Objective:
The objective of this cooperative research project is to identify molecular markers for the genes for Stemphylium blight resistance and rust, determine the epidemiology of the Stemphylium blight and Uromyces rust pathogens, and initiate marker-assisted selection for Stemphylium blight and rust resistance in lentil that can be readily adopted by breeding programs.

Approach:
Develop mapping populations of recombinant inbred lines (RILS) from crosses of rust and Stemphylium blight resistant parents with susceptible parents. Phenotype the mapping populations for reaction to rust and blight. Use bulk segregant analysis (BSA) to identify candidate markers (AFLP and SSR) for the genes for resistance to Stemphylium blight and rust. Develop a molecular map for the populations using AFLP and SSR markers. Where appropriate convert candidate markers to Sequence Characterized Amplified Regions (SCARs) that can be used efficiently in marker assisted selection. Determine the nature, distribution, and life cycles of Stemphylium blight and Uromyces rust causing pathogens. Determine environmental conditions favorable to disease development and spread of Stemphylium blight and screening protocols (both field and controlled environments) for identifying Stemphylium blight and Uromyces rust resistance in lentil germplasm and breeding lines. Formerly 5348-21000-014-12T (5/08).